Abstract
The antipodal cells have been the stepchildren in most investigations of the female gametophyte. In Hordeum vulgare cv. Bomi, three antipodal cells are originally developed chalazally but because of differential growth of the embryo sac they soon become laterally situated and their number increases to 35–50 cells and the shape, size and structure of the cells change in the time before as well as after fertilization. The cells persist until about 60–70 h after pollination. At that time, the embryo consists of about 12–15 cells and a cellularization of the nuclear endosperm has started peripherally. The size of nuclei, and especially nucleoli, in the antipodal cells increases tremendously in the investigated period and the amounts of organelles also change. The walls of antipodals are diversified depending on which cells they are separating, and wall invaginations are developed especially between antipodal cells and surrounding nucellar cells in the placental region. The mitochondria appear in various shapes in section view, very often as cups or dumbbells with a rim in the ends containing cristae and a thin cristae-free base. These bases are sometimes stretched out as thin parts and at last a simple parting occurs. Binary fissions of the plastids happen especially in the hours before and just after egg fertilization. ER is extraordinarily well developed in the whole period of investigation and many ribosomes are attached to the membranes. Dictyosomes form numerous vesicles, especially in the antipodals near the nucellar cells in the placental region. These ultrastructural details support the opinion that antipodal cells may play an important role in the embryo sac and are able to be responsible for the supply of nutrition for the whole gametophyte and take part in the supply of nutrition during embryo formation.
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Engell, K. Embryology of barley. IV. Ultrastructure of the antipodal cells of Hordeum vulgare L. cv. Bomi before and after fertilization of the egg cell. Sexual Plant Reprod 7, 333–346 (1994). https://doi.org/10.1007/BF00230512
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DOI: https://doi.org/10.1007/BF00230512