Summary
The survival of isolated sperm cells from maize pollen grains at 20° C and their cryopreservation were studied by means of the fluorochromatic reaction (FCR) test. An osmotic pressure of 500 mOsmol, a pH of 7 adjusted with MOPS buffer, and the ten-fold dilution of Brewbacker and Kwack salts enabled isolated sperm cells to survive for a maximum of 35 h at 20° C. The addition of 3 mM of calcium chloride or 0.1 % glucose also slightly improved survival. The addition of 1% glutamine gave the best percentage of FCR-positive cells at 20 h. The addition of all of these substances in a unique medium provoked the formation of spermcell aggregates that were FCR positive. With respect to cryopreservaton, 70% of the isolated sperm cells remained FCR positive after freezing at -80° C and quick thawing at 37° C in the selected survival medium.
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Roeckel, P., Dumas, C. Survival at 20° C and cryopreservation of isolated sperm cells from Zea mays pollen grains. Sexual Plant Reprod 6, 212–216 (1993). https://doi.org/10.1007/BF00228651
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DOI: https://doi.org/10.1007/BF00228651