Summary
Synthetic alloploid Brassica oxyrrhina (2n = 18, OO) x B. campestris (2n = 20, AA) was repeatedly backcrossed with B. campestris to place B. campestris nucleus in the cytoplasm of B. oxyrrhina. Alloplasmic plants, obtained in BC5 generation, were stably male sterile but mildly chlorotic during initial development. Synthetic alloploid B. oxyrrhina-campestris was also hybridized with B. juncea to transfer B. oxyrrhina cytoplasm. Segregation for green and chlorotic plants was observed in BC1 and BC2 generations. By selection, however, normal green male sterile B. juncea was obtained in BC3. Pollen abortion in both B. campestris and B. juncea is post-meiotic.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bannerot H, Boulidard L, Cauderon Y, Tempe J (1974) Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica olerecea. In: Proc EUCARPIA Meeting, Dundee. Scotland, pp 52–54
Bannerot H, Boulidard L, Cheepeau Y (1977) Unexpected difficulties met with the radish cytoplasm. Cruciferae Newslett 2:16
Hinata K, Konno N (1979) Studies on a male-sterile strain having the Brassica campestris nucleus and the Diplotaxis muralis cytoplasm. I. On the breeding procedures and some characteristics of the male sterile strain. Jpn J Breed 29:305–311
Jarl CI, Borman CH (1988) Correction of chlorophyll-defective, male sterile winter oilseed rape (Brassica napus) through organelle exchange: Phenotypic evaluation of progeny. Hereditas 108:97–102
Mukai Y, Tsunewaki K (1976) Genetic diversity of the cytoplasm in Triticum and Aegilops. 4. Distribution of the cytoplasm inducing variegation in common wheat. Theor Appl Genet 48:9–16
Ogura H (1968) Studies of a new male-sterility in Japanese radish, with special reference to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima Univ 6:39–78
Paulmann W, Röbbelen G (1988) Effective transfer of cytoplasmic male sterility from radish (Raphanus sativus L.) to rape (Brassica napus L.). Plant Breed 100:299–309
Pearson OH (1972) Cytoplasmically inherited male sterility characters and flavour components from the species cross Brassica nigra (L.) Koch x B. oleracea L. J Am Soc Hortic Sci 97:397–402
Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191:244–250
Prakash S, Chopra VL (1988) Synthesis of alloplasmic Brassica campestris as a new source of cytoplasmic male sterility. Plant Breed 101:253–255
Rousselle P (1982) Premiers resultats d'un programme d'introduction de l'androsterilite' “ogura” du radiz chez le colza. Agronomie 2:859–864
Rousselle P, Renard M (1978) Study of a cytoplasmic male sterility in rapeseed. Cruciferae Newslett 3:40–41
Williams PH, Heyn FW (1981) The origin and development of cytoplasmic male sterile Chinese cabbage. In: Talekar S, Griggs TD (eds) Proc 1st Int Symp on Chinese Cabbage. Asian Vegetable Research Development Centre, Tainan, Taiwan, pp 293–300
Author information
Authors and Affiliations
Additional information
Communicated by K. Tsunewaki
Rights and permissions
About this article
Cite this article
Prakash, S., Chopra, V.L. Male sterility caused by cytoplasm of Brassica oxyrrhina in B. campestris and B. juncea . Theoret. Appl. Genetics 79, 285–287 (1990). https://doi.org/10.1007/BF00225965
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225965