Summary
The effects of a chronic administration of ACTH (up to 36 consecutive days) on the mitochondria of the zona fasciculata of the rat adrenal cortex were investigated by stereologic techniques. It was found that, while the volume of the mitochondrial compartment significantly increases in relation to the duration of treatment, the size and number of mitochondria display a different pattern. Up to the 9th day of hormone treatment mitochondria significantly increase in volume, whereas their number per cell is only slightly increased. After 12 days of ACTH-treatment there is a tremendous increase in the number of organelles per cell, resulting in a significant decrease in their average volume. After 24 and 36 consecutive days of treatment the number of mitochondria per cell as well as their average volume both show a slight but significantly constant increase.
The hypothesis that ACTH controls the processes of growth and division of adrenal mitochondria is discussed in the light of evidence indicating that mitochondria contain a complete genetic apparatus largely independent of nuclear control.
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Armato, U., Nussdorfer, G. G.: Tissue culture of rat adult decapsulated adrenal glands. A methodological, ultrastructural and morphometric investigation. Z. Zellforsch. 185, 245–273 (1972)
Ashwell, M., Work, T. S.: The biogenesis of mitochondria. Ann. Rev. Biochem. 39, 251–290 (1970)
Belt, W. D., Pease, D. C.: Mitochondrial structure in sites of steroid secretion. J. biophys. biochem. Cytol. 2 (Suppl.) 369–374 (1956)
Canick, J. A., Purvis, J. L.: The maintenance of mitochondrial size in the rat adrenal cortex zona fasciculata by ACTH. Exp. molec. Path. 16, 79–93 (1972)
Christensen, A. K.: The fine structure of testicular interstitial cells in guinea pig. J. Cell Biol. 26, 911–935 (1965)
Christensen, A. K., Gillim, S. W.: The correlation of fine structure and function in steroid secreting cells, with emphasis on those of the gonads. In: The gonads (K. W. McKerns, ed.). 1st ed., p. 415–488. Amsterdam: North Holland Publishing Co. 1969
Clark-Walker, G. D., Linnane, A. W.: The biogenesis of mitochondria in Saccharomyces cerevisiae. A comparison between cytoplasmic respiratory-deficient mutant yeast and chloramphenicol-inhibited wild type cells. J. Cell Biol. 34, 1–14 (1967)
Deane, H. W.: The anatomy, chemistry, and physiology of adrenocortical tissue. In: Handbuch der experimentellen Pharmakologie (O. Eichler and A. Farah, eds.), 1st ed., vol. 14, part 1, p. 1–185. Berlin-Göttingen-Heidelberg: Springer 1962
De Hoff, R. I., Rhines, F. R.: Determination of the number of particles per unit volume from measurements made on random plane sections: the general cylinder and the ellipsoid. Trans. Amer. Inst. Minning. Met. Petrol. Engrs. 221, 975–987 (1961)
De Nicola, A. F.: Effect of chronic treatment with ACTH on 11 β-hydroxylation in rat adrenal mitochondria. Acta physiol. lat.-amer. 23, 178–182 (1973)
De Nicola, A. F., Freire, F.: Mitochondrial 11 β-hydroxylation and adrenal morphology in rats bearing a mammotropic, ACTH-secreting pituitary tumor. J. Steroid Biochem. 4, 407–416 (1973)
Dexter, R. N., Fishman, L. H., Ney, R. L.: Stimulation of adrenal cholesterol uptake from plasma by adrenocorticotrophin. Endocrinology 87, 836–846 (1970)
Dexter, R. N., Fishman, L. H., Ney, R. L., Liddle, G. W.: An effect of adrenocorticotrophic hormone on adrenal cholesterol accumulation. Endocrinology 81, 1185–1187 (1967)
Dorfman, R. I., Ungar, F.: Metabolism of steroid hormones, 1st ed., New York: Academic Press 1965
Farese, R. V.: Regulation of the adrenal growth and steroidogenesis by ACTH. In: Functions of the adrenal cortex (K.W. McKerns, (ed.), 1st ed., p. 539–581. Amsterdam: North Holland Publishing Co. 1968)
Frühling, J., Sand, G., Penasse, W., Pecheux, F., Claude, A.: Corrélation entre la morphologie et le contenu lipidique des corticosurrénales du cobaye, du rat et du boeuf. J. Ultrastruct. Res. 44, 113–133 (1973)
Griffiths, K., Glick, D.: Determination of the 11 β-hydroxylase activity in microgram samples of tissue: its quantitative histological distribution in the rat adrenal and the influence of corticotrophin. J. Endocr. 35, 1–12 (1966)
Guttes, E. W., Hanawalt, P. C., Guttes, S.: Mitochondrial DNA synthesis and the mitotic cycle in Physarium policephalum. Biochim. biophys. Acta (Amst.) 142, 181–194 (1967)
Ichii, S., Kobayashi, S., Yago, N., Omata, S.: Role of plasma cholesterol in rat adrenal corticosteroidogenesis in vitro. Endocr. jap. 14, 138–142 (1967)
Idelman, S.: Ultrastructure of the mammalian adrenal cortex. Int. Rev. Cytol. 27, 181–282 (1970)
Kahri, A.: Histochemical and electron microscopic studies on cells of the rat adrenal cortex in tissue culture. Acta endocr. (KM) 52 (Suppl. 108), 1–96 (1966)
Kahri, A. I.: Inductive transformation as a basic phenomenon in the genesis of cells of the zona fasciculata and reticularis of the adrenal cortex. Acta anat. (Basel) 71, 67–78 (1968)
Kahri, A. I.: Selective inhibition by chloramphenicol of ACTH-induced reorganization of inner mitochondrial membranes in fetal adrenal cortical cells in tissue culture. Amer. J. Anat. 127, 103–130 (1970)
Kahri, A. I.: Inhibition of ACTH-induced differentiation of cortical cells and their mitochondria by corticosterone in tissue culture of fetal rat adrenals. Anat. Rec. 176, 253–272 (1973)
Karnovsky, M. J.: Simple method for staining with lead at high pH in electron microscopy. J. biophys. biochem. Cytol. 11, 729–732 (1961)
Kimura, T.: Effects of hypophysectomy and ACTH administration on the level of adrenal cholesterol side-chain desmolase. Endocrinology 85, 492–499 (1969)
Kowal, J.: The effect of ACTH and 3′,5′-AMP on steroidogenesis and 11 β-hydroxylation in cultured adrenal cells. Clin. Res. 15, 262 (1967)
Kowal, J.: Adrenal cells in tissue culture. IV. Use of an inhibitor of steroid synthesis for the study of ACTH-action. Endocrinology 85, 270–279 (1969)
Lockwood, W. R.: A reliable and easily sectioned epoxy embedding medium. Anat. Rec. 50, 129–140 (1964)
Loud, A. V.: A method for the quantitative estimation of cytoplasmic structures. J. Cell Biol. 15, 481–87 (1962)
Magalhāes, M. C., Magalhāes, M. M.: A stereologic study of the effects of metopiron on the rat adrenal. Lab. Invest. 21, 491–496 (1969)
Me Kerns, K. W.: Mechanism of ACTH regulation of the adrenal cortex. In: Functions of the adrenal cortex (K. W. McKerns, ed.), 1st ed., p. 479–537. Amsterdam: North Holland Publishing Co. 1968
Merkle, U.: Histologische Studie zur Morphokinese der Nebennierenrinde. Z. mikr.-anat. Forsch. 79, 460–476 (1968)
Millonig, G.: Advantage of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physiol. 32, 1637 (1961)
Milner, A. J.: Corticotrophin-induced differentiation of mitochondria in rat adrenal cortical cells grown in primary tissue culture: effects of ethidium bromide. J. Endocr. 52, 541–548 (1972a)
Milner, A. J.: Cyclic AMP and the differentiation of adrenal cortical cells grown in tissue culture. J. Endocr. 55, 405–413 (1972b)
Milner, A. J.: Morphological differentiation of adrenal cortical cells induced by a 40-minute exposure to corticotrophin. J. Endocr. 56, 325–326 (1973)
Milner, A. J., Villee, D. B.: Steroidogenic and morphologic effects of ACTH on human foetal adrenal cells grown in tissue culture. Endocrinology 87, 596–601 (1970)
Mitro, A., Palkovitz, H.: Karyometric studies on the reactivity of the adrenal cortex of newborn rats. Acta endocr. (Kbh) 63, 385–391 (1970)
Moses, H. L., Davis, W. W., Rosenthal, A. S., Garren, L. D.: Adrenal cholesterol: localization by electronmicroscope autoradiography. Science 163, 1203–1205 (1969)
Neupert, W., Brdiczka, D., Bücher, Th.: Incorporation of aminoacids into the outer and inner membrane of isolated rat liver mitochondria. Biochem. biophys. Res. Commun. 27, 488–493 (1967)
Neupert, W., Brdiezka, D., Bücher, Th.: Incorporation of aminoacids into the outer and inner membrane of isolated rat liver mitochondria. In: Biochemical aspects of the biogenesis of mitochondria (E. C. Slater, J. M. Tager, S. Papa, and E. Quagliariello, eds), 1st ed., p. 395–407. Bari (Italy): Adriatica Editrice 1968
Nishikawa, M., Murone, I., Sato, T.: Electron microscopic investigation of the adrenal cortex. Endocrinology 72, 197–209 (1963)
Nussdorfer, G. G.: Fine structure of the newborn rat adrenal cortex. I. Zona glomerulosa and zona fasciculata. Z. Zellforsch. 103, 382–397 (1970a)
Nussdorfer, G. G.: Effects of corticosteroid-hormones on the smooth endoplasmic reticulum of rat adrenocortical cells. Z. Zellforsch. 106, 143–154 (1970b)
Nussdorfer, G. G., Mazzocchi, G.: Correlated morphometric and autoradiographic studies on the effects of corticosterone on adrenocortical cells of intact and hypophysectomized ACTH-treated rats. Z. Zellforsch. 111, 90–105 (1970)
Nussdorfer, G. G., Mazzocchi, G.: Effect of ACTH on mitochondrial RNA synthesis of rat adrenocortical cells. Z. Zellforsch. 118, 35–48 (1971a)
Nussdorfer, G. G., Mazzocchi, G.: Effects of corticosterone on nuclear and mitochondrial DNA-dependent protein synthesis of adrenocortical cells of hypophysectomized ACTH treated rats. Steroidologia 2, 244–256 (1971b)
Nussdorfer, G. G., Mazzocchi, G.: A stereologic study of the effects of ACTH and cyclic 3′,5′-AMP on adrenocortical cells of intact and hypophysectomized rats. Lab. Invest. 26, 45–52 (1972a)
Nussdorfer, G. G., Mazzocchi, G.: Effect of 3′∶5′-cyclic AMP on nuclear and mitochondrial protein synthesis by adrenocortical cells. Acta endocr. (Kbh) 70, 81–88 (1972b)
Nussdorfer, G. G., Mazzocchi, G.: Effects of 3′,5′-cyclic nucleotides on adrenocortical cells of hypophysectomized rats. A stereologic and autoradiographic study. Lab. Invest. 28, 332–342 (1973a)
Nussdorfer, G. G., Mazzocchi, G.: Further studies on the trophic effect of cyclic GMP on rat adrenal cortex. J. Endocr. 57, 559–560 (1973b)
Nussdorfer, G. G., Mazzocchi, G., Rebonato, L.: Long-term trophic effect of ACTH on rat adrenocortical cells. An ultrastructural, morphometric and autoradiographic study. Z. Zellforsch. 115, 30–45 (1971)
Nussdorfer, G. G., Mazzocchi, G., Rebuffat, P.: An ultrastructural stereologic study of the effects of ACTH and adenosine 3′,5′-cyclic monophosphate on the zona glomerulosa of rat adrenal cortex. Endocrinology 92, 141–151 (1973)
Nussdorfer, G. G., Mazzocchi, G., Rebuffat, P.: An ultrastructural stereologic study of the effects of 3′∶5′-cyclic nucleotides on the zona glomerulosa of rat adrenal cortex. Endocrinology 94, 602–608 (1974)
O'Hare, M. J., Neville, A. M.: Morphological responses to corticotrophin and cyclic AMP by adult rat adrenocortical cells in monolayer culture. J. Endocr. 56, 529–536 (1973)
Pfeiffer, D. R., Chu, J. W., Kuo, T. H., Chan, S. W., Kimura, T., Tchen, T. T.: Changes in some biochemical parameters including cytochrome P-450 after hypophysectomy and their restoration by ACTH administration in rats four months post hypophysectomy. Biochem. biophys. Res. Commun. 48, 486–490 (1972)
Robinson, G. A., Butcher, R. W., Sutherland, E. R.: Cyclic AMP, 1st ed. New York: Academic Press 1971
Roodyn, D. B.. Wilkie, D.: The biogenesis of mitochondria, 1st ed. London: Methuen & Co. 1968
Sabatini, D. D., Bensch, K., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963)
Sabatini, D. D., De Robertis, E. D., Bleichmar, H. B.: Submicroscopic study of the pituitary action on the adrenocortex in the rat. Endocrinology 70, 390–406 (1962)
Sand, G., Frühling, J., Penasse, W., Claude, A.: Distribution du cholestérol dans la corticosurrénale du rat: analyse morphologique et chimique des fractions subcellulaires, isolées par centrifugation différentielle. J. Microscopic 15, 41–66 (1972)
Schwartz, H. A.: The metallographic determination of size distribution of temper carbon nodules. Metals and Alloys 5, 139–140 (1934)
Sekiyama, S., Yago, N., Iwai, Y., Kurokawa, H., Sato, F., Shiragai, A.: A stereological description of size and number of zona fasciculata mitochondria in rat adrenal cortex after hypophysectomy. Endocr. jap. 18, 365–369 (1971)
Sharawy, M., Penney, D. P.: Unusual mitochondrial morphology in the rat adrenal cortex following hypophysectomy. Amer. J. Anat. 136, 395–401 (1973)
Sharma, R. K., Hashimoto, K., Kitabchi, A. E.: Steroidogenesis in isolated adrenal cells of rat. III. Morphological and biochemical correlation of cholesterol and cholesterol ester content in ACTH and N 6–2′-0-dibutyryl adenosine-3′,5′ monophosphate activated adrenal cells. Endocrinology 91, 994–1003 (1972)
Shelton, J. H., Jones, A. L.: The fine structure of the mouse adrenal cortex and the ultrastructural changes in the zona glomerulosa with low and high sodium diets. Anat. Rec. 170, 147–182 (1971)
Tamaoki, B.-I: Steroidogenesis and cell structure. Biochemical pursuit of sites of steroid biosynthesis. J. Steroid Biochem. 4, 89–118 (1973)
Tandler, B., Erlandson, R. A., Smith, A. L., Wynder, E. L.: Riboflavin and mouse hepatic cell structure and function. II. Division of mitochondria during recovery from simple deficiency. J. Cell Biol. 41, 477–493 (1969)
Trump, B. F., Smuckler, E. A., Benditt, E. P.: A method for staining epoxy sections for light microscopy. J. Ultrastruct. Res. 5, 343–348 (1961)
Tzagoloff, A., Rubin, M. S., Sierra, M. F.: Biosynthesis of mitochondrial enzymes. Biochim. biophys. Acta (Amst.) 301, 71–104 (1973)
Volk, T. L., Scarpelli, D. G.: Mitochondrial gigantism in the adrenal cortex following hypophysectomy. Lab. Invest. 15, 707–714 (1966)
Weibel, E. R.: Stereological principles for morphometry in electron microscopic cytology. Int. Rev. Cytol. 26, 235–302 (1969)
Yago, N., Seki, M., Sekiyama, S., Kobayashi, S., Kurokawa, H., Iwai, Y., Sato, F., Shiragai, A.: Growth and differentiation of mitochondria in the regenerating rat adrenal cortex. A correlated biochemical and stereological approach. J. Cell Biol. 52, 503–513 (1972)
Yago, N., Sekiyama, S., Kurokawa, H., Iwai, Y., Sato, F., Shiragai, A.: Volume increase in rat adrenocortical mitochondria by single ACTH administration. A stereologic study. Endocr. jap. 18, 359–364 (1971)
Yu, R., Lukins, H. B., Linnane, A. W.: Selective in vivo action of cycloheximide on the synthesis of soluble mitochondrial proteins. In: Biochemical aspects of the biogenesis of mitochondria (E. C. Slater, J. M. Tager, S. Papa, and E. Quagliariello, eds), 1st ed., p. 359–364. Bari (Italy): Adriatica Editrice 1968
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A preliminary report on part of this material was given at the IX Congress of the Società Italiana di Microscopia Elettronica, St. Vincent, October 1973.
Authors wish to thank Mr. G. Gottardo and Miss A. Coi for their excellent technical assistance. This work was partly supported by a contract with the CNR (69.0172/115.3439 and C. T. 73.00663.04).
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Nussdorfer, G.G., Rebuffat, P., Mazzocchi, G. et al. Investigations on adrenocortical mitochondria turnover. Cell Tissue Res. 150, 79–94 (1974). https://doi.org/10.1007/BF00220382
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DOI: https://doi.org/10.1007/BF00220382