Summary
The response of rat gastrocnemius muscle fibers to chronic streptozotocin-diabetes was studied. Transverse sections of this muscle from normal and diabetic rats were histochemically assayed for reduced diphosphopyridine nucleotide-diaphorase, myofibrillar adenosine triphosphatase, mitochondrial alpha-glycerophosphate dehydrogenase, beta-hydroxybutyrate dehydrogenase, and alkaline phosphatase activities. Cross-sectional areas of the fiber types were measured, and fiber capillarization and populations estimated. Chemically-induced diabetes appeared to have little effect on the metabolic or morphological properties of slow-twitch fibers. However, a general dedifferentiation occurred in the 2 fast-twitch fiber populations. There was a loss of oxidative potential in the fast-twitch-oxidative-glycolytic fibers, and a significant decrease in size in the fast-twitch-glycolytic fibers. No change in the proportions of slow- and fast-twitch fibers in the muscles of diabetic rats occurred. It is concluded that hypoinsulinism has differential effects on the 3 fiber types in heterogeneous rat skeletal muscle, and that slow-twitch fibers are least affected by the diabetic condition.
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Ariano, M., Armstrong, R. B., Edgerton, V. R.: Hindlimb muscle fiber populations of five mammals. J. Histochem. Cytochem. 21, 51–55 (1973)
Baldwin, K. M., Klinkerfuss, G. H., Terjung, R. L., Molé, P. A., Holloszy, J. O.: Respiratory capacity of white, red, and intermediate muscle: adaptive response to exercise. Amer. J. Physiol. 222, 373–378 (1972)
Gillespie, C. A., Simpson, D. R., Edgerton, V. R.: High glycogen content of red as opposed to white skeletal muscle fibers of guinea pig. J. Histochem. Cytochem. 18, 552–558 (1970)
Gollnick, P. D., Ianuzzo, C. D.: Hormonal deficiencies and the metabolic adaptations of rats to training. Amer. J. Physiol. 223, 278–282 (1972)
Gomori, G.: Microtechnical demonstration of phosphatase in tissue sections. Proc. Soc. exp. Biol. (N. Y.) 42, 23–26 (1939)
Ianuzzo, C. D., Lesser, M., Battista, P.: Metabolic adaptations of streptozotocin-diabetic rats to exercise training. Biochem. biophys. Res. Commun. 58, 107–111 (1974)
Kark, R. A. F., Blass, J. P., Avigon, J., Engel, W. K.: The oxidation of beta-hydroxybutyric acid by small quantities of type-pure red and white skeletal muscles. J. biol. Chem. 246, 4560–4566 (1971)
Mai, J. V., Edgerton, V. R., Barnard, R. J.: Capillarity of red, white, and intermediate muscle fibers in trained and untrained guinea pigs. Experientia (Basel) 26, 1222–1223 (1970)
Manchester, K. L.: The control by insulin of amino acid accumulation in muscle. Biochem. J. 117, 457–465 (1970)
Novikoff, A.B., Shin, W., Drucker, J.: Mitochondrial localization of oxidation enzymes: staining results with two tetrazolium salts. J. biophys. biochem. Cytol. 9, 47–61 (1961)
Padykula, H. A., Herman, E.: The specificity of the histochemical method of adenosine triphosphatase. J. Histochem. Cytochem. 3, 170–195 (1955)
Peter, J. B., Barnard, R. J., Edgerton, V. R., Gillespie, C. A., Stempel, K. E.: Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits. Biochemistry (Wash.) 11, 2627–2633 (1972)
Randle, P. J.: Carbohydrate metabolism and lipid storage and breakdown in diabetes. Diabetologia 2, 237–247 (1966)
Romanul, F. C. A., Hogan, E. L.: Enzymatic changes in denervated muscle. Arch. Neurol. (Chic.) 13, 263–273 (1965)
Saubert, C. W., IV., Armstrong, R. B., Shepherd, R. E., Gollnick, P. D.: Anaerobic enzyme adaptations to sprint training in rats. Pflügers Arch. 341, 305–312 (1973)
Thomas, P. K., Lascelles, R. G.: Schwann-cell abnormalities in diabetic neuropathy. Lancet 1, 1355–1357 (1965)
Wattenberg, L. W., Leong, J. L.: Effects of coenzyme Q10 and menadione on succinate dehydrogenase activity as measured by tetrazolium salt reduction. J. Histochem. Cytochem. 8, 296–303 (1960)
Wool, I. G., Stirewalt, W. S., Kurihara, K., Low, R. B., Bailey, P., Oyer, D.: II. Hormones and metabolic function—Mode of action of insulin in the regulation of protein biosynthesis in muscle. Recent Progr. Hormone Res. 24, 139–208 (1968)
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Armstrong, R.B., Gollnick, P.D. & Ianuzzo, C.D. Histochemical properties of skeletal muscle fibers in streptozotocin-diabetic rats. Cell Tissue Res. 162, 387–393 (1975). https://doi.org/10.1007/BF00220185
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DOI: https://doi.org/10.1007/BF00220185