Summary
Lysozyme is a bacteriolytic enzyme found in respiratory tract fluid. In this study, immunocytochemistry was used to determine the cells of origin of tracheal lysozyme in the ferret. Lysozyme was found in secretory granules of serous but not mucous cells in the submucosal glands, and was absent from the surface epithelium, cartilage, and connective tissue. The exclusive presence of lysozyme in serous gland cells renders it useful as a biochemical marker of that cell type.
Measurements of lysozyme assayed from the incubating medium indicated that bethanechol stimulated lysozyme release by 260±80.9% (mean ±SE), phenylephrine by 80±16.4%, and terbutaline by 25±10.2%. Electron-microscopic and immunocytochemical analysis of incubated tissues revealed loss of serous granules and lysozyme immuno-reactivity in response to the drugs. Atropine, propranolol, and phentolamine blocked the stimulatory effects of bethanechol, terbutaline, and phenylephrine, respectively.
These findings establish the usefulness of lysozyme as a serous-cell marker and demonstrate that secretory responses of different magnitude are evoked by equimolar concentrations of alpha- and beta-adrenergic and cholinergic drugs.
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References
Arnheim N (1971) Immunochemical resemblance between human leukemia and hen egg-white lysozyme and their reduced carboxymethyl derivatives. J Mol Biol 61:237–250
Arnon R (1977) Immunochemistry of lysozyme. In: RS Milton (eds) Immunochemistry of enzymes and their antibodies. John Wiley & Sons, New York, pp 1–28
Basbaum CB, Ueki I, Brezina L, Nadel JA (1981) Tracheal submucosal gland serous cells stimulated in vitro with adrenergic and cholinergic agonists: a morphometric study. Cell Tissue Res 220:481–498
Boat T, Kleinerman J (1975) Human respiratory tract secretions. II. Effect of cholinergic and adrenergic agents on in vitro release of protein and mucous glycoprotein. Chest 67:32s-34s
Borson DB, Chinn RA, Davis B, Nadel JA (1980) Adrenergic and cholinergic nerves mediate fluid secretion from tracheal glands of ferrets. J Appl Physiol 49:1027–1031
Bowes D, Corrin B (1977) Ultrastructural immunocytochemical localisation of lysozyme in human bronchial glands. Thorax 32:163–170
Glynn AA, Parkman R (1964) Studies with an antibody to rat lysozyme. Immunology 7:724–729
Heitz PV, Wegmann W (1980) Identification of neoplastic Paneth cells in an adenocarcinoma of the stomach using lysozyme as a marker, and electron microscopy. Virchows Arch Pathol Anat 386:107–116
Jacob S, Poddar S (1981) Mucus cells of the tracheobronchial tree in the ferret. J Anat 133:691
Klockars M, Reitamo S (1975) Tissue distribution of lysozyme in man. J Histochem Cytochem 23:932–940
Konstan MW, Chen PW, Sherman JM, Thomassen MJ, Wood RE, Boat TF (1981) Human lung lysozyme: sources and properties. Am Rev Respir Dis 123:120–124
Liedtke CM, Boat TF, Rudolph SA (1982) Beta-adrenergic agonists increase cAMP levels and stimulate mucous glycoprotein secretion in cat tracheal explants. Chest 81:20S-21S
Mason DY, Taylor CR (1975) The distribution of muramidase (lysozyme) in human tissues. J Clin Res 28:124–132
Osserman EF, Lawlor DP (1966) Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med 124:921–951
Phipps RJ (1979) Adrenergic stimulation of mucus secretion in the human bronchus. J Physiol (Lond) 296:44P
Phipps RJ, Williams I, Pack R, Richardson P (1982) Adrenergic stimulation of mucus secretion in human bronchi. Chest 81:195–205
Rodbard D (1974) Statistical quality control and routine data processing for radioimmunoas-says and immunoradiometric assays. Clin Chem 20:1255–1270
Spicer SS, Frayser R (1977) Immunocytochemical localization of lysozymes in respiratory and other tissues. Lab Invest 36:282–295
Sternberger LA (1974) Immunocytochemistry. Prentice Hall, Inc, Englewood Cliffs, NJ, pp 129–172
Ueki I, German V, Nadel J (1980) Micropipette measurement of airway submucosal gland secretion: autonomic effects. Am Rev Respir Dis 121:351–357
Wilson AC, Prager EM (1974) Antigenic comparison of animal lysozymes. In: EF Osserman, RE Canfield, S Beychok (eds) Lysozyme. Academic Press, Inc, New York, pp 127–141
Zar JH (1974) Biostatistical Analysis. Prentice-Hall, Inc, New Jersey
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Tom-Moy, M., Basbaum, C.B. & Nadel, J.A. Localization and release of lysozyme from ferret trachea: Effects of adrenergic and cholinergic drugs. Cell Tissue Res. 228, 549–562 (1983). https://doi.org/10.1007/BF00211475
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DOI: https://doi.org/10.1007/BF00211475