Summary
The application of confocal laser scanning microscopy together with in situ hybridization experiments in tobacco pollen enabled a detailed localization of a pollen-specific mRNA. The three-dimensional distribution of this specific mRNA over the whole pollen grain was reconstructed by means of optical sections of one specimen.
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Reijnen, W.H., van Herpen, M.M.A., de Groot, P.F.M. et al. Cellular localization of a pollen-specific mRNA by in situ hybridization and confocal laser scanning microscopy. Sexual Plant Reprod 4, 254–257 (1991). https://doi.org/10.1007/BF00200544
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DOI: https://doi.org/10.1007/BF00200544