Summary
Glucan production by Botrytis cinerea increased from 1 g/l to 3 g/l when KNO3 or urea replaced asparagine as the nitrogen source. A further enhancement up to 5 g/l was obtained with nitrogen-limited medium or non-growing cells. Under these conditions an extracellular glucan layer was attached to the mycelium. The adherent glucan made up 60% of the total amount of glucan produced and thus increased the total glucan yield to 13 g/l. An enzymatic analysis of the adherent glucan indicated that only about every fifth molecule of the main chain was substituted by a glucose unit. In contrast, in the free glucan of culture filtrates glucose units were distributed at approximately every second to third residue of the main chain.
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Pielken, P., Stahmann, P. & Sahm, H. Increase in glucan formation by Botrytis cinerea and analysis of the adherent glucan. Appl Microbiol Biotechnol 33, 1–6 (1990). https://doi.org/10.1007/BF00170559
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DOI: https://doi.org/10.1007/BF00170559