Summary
The fungal micro-organism Trichoderma reesei was grown in batch culture with excess glucose at pH values between 2.7 and 4.5 and temperatures between 25°C and 35°C. A maximum lipid concentration of 16.9% of the cell dry weight was achieved at pH 3.2 and a temperature of 27°C. Lipid concentration was shown to be correlated with a calculated maximum specific growth rate (µ mc ) and the maximum lipid value occurred at µ mc = 0.10 h−1. Fatty acid analysis was carried out and found to change with changing pH and temperature. Palmitic (16:0) acid and unusually high proportions of stearic acid (18:0) were commonly present. A conversion of fatty acids to palmitoleic acid (16:1) occurred following an unidentified nutrient limitation other than nitrogen depletion after 70 h of culture.
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Brown, D.E., Hasan, M., Lepe-Casillas, M. et al. Effect of temperature and pH on lipid accumulation by Trichoderma reesei . Appl Microbiol Biotechnol 34, 335–339 (1990). https://doi.org/10.1007/BF00170054
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DOI: https://doi.org/10.1007/BF00170054