Summary
Polysaccharide formation by Aureobasidium pullulans was affected by the nitrogen source in the medium, and its yield fell when excess ammonium ions were present, even under conditions which otherwise supported its synthesis. Experiments carried out with pH control gave results which question some of the earlier published data, so it is possible to obtain high polysaccharide yields at low pH (2.5), and mycelial growth at high pH (6.5) with certain nitrogen sources. There appeared to be no causal relationship between morphology and polysaccharide-producing capacity of the cultures.
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References
Aharonowitz Y (1980) Nitrogen metabolite regulation of antibiotic biosynthesis. Ann Rev Microbiol 34:209–233
Bulmer MA, Catley BJ, Kelley PJ (1987) The effect of ammonium ions and pH on the elaboration of the fungal extracellular polysaccharide, pullulan by Aureobasidium pullulans. Appl Microbiol Biotechnol 25:362–365
Catley BJ (1971a) Utilization of carbon sources by Pullularia pululans for the elaboration of extracellular polysaccharides. Appl Microbiol 22:641–649
Catley BJ (1971b) Role of pH and nitrogen limitation in the elaboration of the extracellular polysaccharide pullulan by Pullularia pullulans. Appl Microbiol 22:650–654
Catley BJ (1980) The extracellular polysaccharide pullulan produced by Aureobasidium pullulans: a relationship between elaborated rate and morpohology. J Gen Microbiol 120:265–268
Catley BJ (1983) Pullulan synthesis by Aureobasidium pullulans. In: Berkely RCW, Gooday G, Ellwood DC (eds) Microbial polysaccharides and polysaccharases. Academic Press, London, pp 69–84
Catley BJ, Ramsay A, Servis C (1986) Observations on the structure of the fungal extracellular polysaccharide, pullulan. Carbohydr Res 153:79–86
Chul Shin Y, Kim YH, Lee HS, Cho SJ, Byun SM (1989) Production of exopolysaccharide pullulan from inulin by a mixed culture of Aureobasidium pullulans and Kluveromyces fragilis. Biotechnol Bioeng 33:129–133
Evans CT, Ratledge C (1984) Effect of nitrogen source on lipid accumulation in oleaginous yeasts. J Gen Microbiol 130:1693–1704
Heald PJ, Kristiansen B (1985) Synthesis of polysaccharide by yeast-like forms of Aureobasidium pullulans. Biotechnol Bioeng 27:1516–1519
Kristiansen B, Charley RC, Seviour R, Harvey L, Habeeb S, Smith JE (1983) Overproduction of metabolites by filamentous fungi. In: Krumpanzl V, Sityka B, Vanek Z (eds) Overproduction of microbial products. Academic Press, London, pp 195–210
Kusnan MB, Berger MG, Fock HP (1987) The involvement of glutamine synthetase/glutamate synthase in ammonia assimilation by Aspergillus nidulans. J Gen Microbiol 133:1235–1242
Lacroix C, LeDuy A, Noel G, Choplin N (1985) Effect of pH on the batch fermentation of pullulan from sucrose medium. Biotechnol Bioeng 27:202–207
LeDuy A, Zajic JE, Luong JHT, Choplin L (1988) Pullulan. In: Mark HF, Bikales NM, Overberger CG, Menges G (eds) Encyclopaedia of Polymer Science and Engineering, 2nd edn, Wiley New York, pp 650–660
Martin JF, Demain AL (1978) Fungal development and metabolite formation. In: Smith JE, Berry DR (eds) The filamentous fungi, vol 3. Developmental mycology. Edward Arnold, London, pp 426–450
McNeil B, Kristiansen B (1987) Influence of impeller speed upon the pullulan fermentation. Biotechnol Lett 9:101–104
McNeil B, Kristiansen B, Seviour RJ (1989) Polysaccharide production and morphology of Aureobasidium pullulans in continuous culture. Biotechnol Bioeng 33:1210–1212
Ono K, Yasuda N, Ueda S (1977) Effect of pH on pullulan elaboration by Aurebasidium pullulans S-1. Agric Biol Chem 41:2113–2118
Pitt DE, Bull AT (1982) The adenine nucleotide composition of growing and stressed cultures of Trichoderma aureoviride. Exp Mycol 6:41–51
Rho D, Mulchandani A, Luong JHT, LeDuy A (1988) Oxygen requirement in pullulan fermentation. Appl Microbiol Biotechnol 28:361–366
Seviour RJ, Kristiansen B (1983) Effect of ammonium ion concentration on polysaccharide production by Aureobasidium pullulans in batch culture. Eur J Appl Biotechnol 17:178–181
Seviour RJ, Kristiansen B, Harvey L (1984) Morphology of Aureobasidium pullulans during polysaccharide elaboration. Trans Br Mycol Soc 82:350–356
Souw P, Demain AL (1979) Nutritional studieson xanthan production by Xanthomonas campestris NRRC B1459. Appl Environ Microbiol 37:1186–1192
Stasinopoulos SJ, Seviour RJ (1989) Exopolysaccharide formation by isolates of Cephalosporium and Acremonium. Mycol Res 92:55–60
Taguchi R, Kikuchi Y, Sakano Y, Kobayashi T (1973) Structural uniformity of pullulan produced by several strains of Pullularia pullulans. Agric Biol Chem 37:1583–1588
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Auer, D.P.F., Seviour, R.J. Influence of varying nitrogen sources on polysaccharide production by Aureobasidium pullulans in batch culture. Appl Microbiol Biotechnol 32, 637–644 (1990). https://doi.org/10.1007/BF00164732
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DOI: https://doi.org/10.1007/BF00164732