Summary
The release of chloramphenicol acetyl transferase (CAT) from a recombinant Escherichia coli strain by ultrasonication and the French press was compared. French pressing disrupted all cells in suspension whereas only a fraction of the cells was disrupted following sonication. The level of CAT released was highest when cells were totally disrupted. Additional treatment with the detergent Triton X-100 was necessary to maximize CAT recovery, presumably due to association of CAT with cellular debris.
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Jorgensen, L., O'Neill, B.K., Thomas, C.J. et al. Release of chloramphenicol acetyl transferase from recombinant Escherichia coli by sonication and the French press. Biotechnol Tech 9, 477–480 (1995). https://doi.org/10.1007/BF00159561
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DOI: https://doi.org/10.1007/BF00159561