Summary
Fluorescence measurement of viable hybridoma cell cultures provides a convenient method for monitoring the progress of a batch culture. It is shown that cell concentration changes as low as 35,000 cells/ml during initial stages of growth can be measured reliably. This sensitivity, however, decreases to 2 × 106 cells/ml at cell concentration greater than 2 × 106 cells/ml. The culture fluorescence of hybridoma culture is a characteristic property of the cell and the medium used. Consequently, processes in which the medium composition and cell lines are invariant, a direct on-line estimate of viable cell count can be made using the method investigated in this paper.
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MacMichael, G., Armiger, W.B., Lee, J.F. et al. On-line measurement of hybridoma growth by culture fluorescence. Biotechnol Tech 1, 213–218 (1987). https://doi.org/10.1007/BF00155457
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DOI: https://doi.org/10.1007/BF00155457