Abstract
Somatic embryos of Cyclamen persicum Mill. could be produced through a callus phase from juvenile explant material including anthers, ovaries and zygotic embryos. The auxin 2,4-D (1.0–1.5 mg l-1) and coconut milk (10% v/v) in MS medium were important factors for the induction of somatic embryogenesis. Somatic embryos germinated into plantlets in MS medium without growth regulators. The plants grew well in the greenhouse and flowered normally. The plants were phenotypically identical to the mother plants with a few exceptions.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthylacetic acid
- IAA:
-
3-indoleacetic acid
- BA:
-
6-benzyladenine
- ABA:
-
abscisic acid
- CM:
-
coconut milk
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Kiviharju, E., Tuominen, U. & Törmälä, T. The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill. Plant Cell Tiss Organ Cult 28, 187–194 (1992). https://doi.org/10.1007/BF00055516
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DOI: https://doi.org/10.1007/BF00055516