Abstract
The major components of protein extracts from the cattle tick Boophilus microplus eggs and larvae of various ages were characterized by molecular sieving chromatography, ion exchange chromatography and SDS-PAGE. The fractions analysed showed a changing chromatographic pattern development. A serum raised against the components of a fraction showing characteristics of vitellin strongly reacted in Western blots with the major peptides of extracts from eggs, larvae, gut and ovary. Comparison of patterns obtained by electrophoresis in non-denaturing PAGE, stained with Coomassie blue or with benzidine/hydrogen peroxide, revealed that the major proteins of these extracts are haemoproteins, possibly in different aggregation states or heterogeneous in composition.
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Canal, C.W., Maia, H.M., Vaz Junior, I.S. et al. Changing patterns of vitellin-related peptides during development of the cattle tick Boophilus microplus . Exp Appl Acarol 19, 325–336 (1995). https://doi.org/10.1007/BF00052390
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DOI: https://doi.org/10.1007/BF00052390