Abstract
High energy state quenching of chlorophyll fluorescence (qE) is inhibited by low concentrations of the inhibitor antimycin A in intact and osmotically shocked chloroplasts isolated from spinach and pea plants. This inhibition is independent of any effect upon ΔpH (as measured by 9-aminoacridine fluorescence quenching). A dual control of qE formation, by ΔpH and the redox state of an unidentified chloroplast component, is implied. Results are discussed in terms of a role for qE in the dissipation of excess excitation energy within photosystem II.
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Abbreviations
- 9-AAmax =:
-
Maximum yield of 9-aminoacridine fluorescence
- DCMU =:
-
3(3,4-dichlorophenyl)-1,1-dimethylurea; Fmax ± Maximum yield of chlorophyll fluorescence
- hr =:
-
hour
- PAR =:
-
Photosynthetically Active Radiation
- QA =:
-
Primary stable electron acceptor within photosystem II
- qE =:
-
High energy state quenching of chlorophyll fluorescence
- qI =:
-
quenching of chlorophyll fluorescence related to photoinhibition
- qP =:
-
Quenching of chlorophyll fluorescence by oxidised plastoquinone
- qQ =:
-
photochemical quenching of chlorophyll fluorescence
- qR =:
-
(Fmax—maximum level of chlorophyll fluorescence induced by the addition of saturating DCMU)
- qT =:
-
Quenching of chlorophyll fluorescence attributable to state transitions
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Oxborough, K., Horton, P. Characterisation of the effects of Antimycin A upon high energy state quenching of chlorophyll fluorescence (qE) in spinach and pea chloroplasts. Photosynth Res 12, 119–127 (1987). https://doi.org/10.1007/BF00047942
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DOI: https://doi.org/10.1007/BF00047942