Abstract
A protocol is presented for the rapid induction of microtubers on micropropagated, layered potato shoots of ‘Kennebec’, ‘Russet Burbank’ and ‘Superior’ in medium devoid of growth regulators. Layered shoots microtuberized more rapidly and produced significantly larger microtubers compared with nodal cuttings. The addition of coumarin or (2-chloroethyl)-trimethylammonium chloride and benzyladenine to microtuberization medium, either had no effect or significantly reduced microtuber weight per shoots compared with medium containing only 80 g × 1-1 sucrose and minimally affected the number of microtubers per shoot. Increasing the incubation period from 28 to 56 days did not affect the number but significantly increased the weight of microtubers per shoot and substantially increased the proportion, up to 20%, of microtubers heavier than 1 gram.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- Ba:
-
benzyladenine
- ccc:
-
(2-chloroethyl) trimethylammonium chloride
- coumarin:
-
2h-1-benzopyran-2-one
- ga3 :
-
gibberellic acid
References
Barker, WG (1953) A method for the in vitro culturing of potato tubers. Science 118: 384
Chandra R, Dodds JH & Tovar P (1988) In vitro tuberization in potato (Solanum tuberosum L.). Newsletter, Intl. Assoc. Plant Tissue Culture. No. 55: 10–20
Chapman, HW (1955) Potato tissue cultures. Amer. Potato J. 32: 207–210
Estrada, RP, Tovar, P & Dodds, JH (1986) Induction of in vitro tubers in a broad range of potato genotypes. Plant Cell Tiss. Org. Cult. 7: 3–10
Ewing, EE (1985) Cuttings as a simplified model of the potato plant. In: Li, PH (Ed) Potato Physiology (pp 153–207). Academic Press, New York
Ewing, EE (1990) Induction of tuberization in potato. In: Vayda, ME & Park, WD (Eds) The Molecular and Cellular Biology of the Potato (pp 25–41). C.A.B. International, Wallinford
Forsline, PL & Langille, AR (1976) An assessment of the modifying effect of kinetin on in vitro tuberization of induced and noninduced tissues of Solanum tuberosum. Can. J. Bot. 54: 2513–2516
Garner, N & Blake, J (1989) The induction and development of potato microtubers in vitro on media free of growth regulating substances. Ann. Bot. 63: 663–674
Gregory, LE (1956) Some factors for tuberization in the potato plant. Ann. Bot. 41: 281–288
Harmey, MA, Crowley, MP & Clinch, PEM (1966) The effect of growth regulators on tuberization of cultured stem pieces of Solanum tuberosum. Eur. Potato J. 9: 146–151
Hussey, G & Stacey, NG (1984) Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.). Ann. Bot. 53: 565–578
Meulemans, M, Dumont, J, Anceau, J & Fouarge, G (1986) In vitro tuberization of potato. Med. Fac. Landbouww. Rijksuniv. Gent. 51/2a: 527–531
Murashige, T & Skoog, F (1962) A revised medium for rapidgrowth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Palmer, CE & Smith, OE (1969) Cytokinins and tuber initiation in the potato Solanum tuberosum L. Nature 221: 279–280
Palmer, CE & Smith, OE (1970) Effect of kinetin on tuber formation on isolated stolons of Solanum tuberosum L. cultured in vitro. Plant Cell Physiol. 11: 303–314
Pelacho, AM & Mingo-Castel, AM (1991) Effects of photoperiod on kinetin-induced tuberization of isolated potato stolons cultured in vitro. Am. Potato J. 68: 533–541
Scherrer, B (1984) Biostatistique. Gaetan Morin Editeur, Chicoutimi, Canada
Stallknecht, GF (1985) Tuber initiation in Solanum tuberosum: effect of phytohormones and induced changes in nucleic acid and protein metabolism. In: Li, PH (Ed) Potato Physiology (pp 231–260). Academic Press, New York
Stallknecht, GF & Farnsworth, S (1982a) General characteristics of coumarin-induced tuberization of axillary shoots of Solanum tuberosum cultured in vitro. Amer. Potato J. 59: 17–32
Stallknecht, GF & Farnsworth, S (1982b) The effect of the inhibitors of protein and nucleic acid synthesis on the coumarin-induced tuberization and growth of excised axillary shoots of potato sprouts (Solanum tuberosum L.) cultured in vitro. Amer. Potato J. 59: 69–75
Steel, RGP & Torrie, JH (1980) Principles and Procedures of Statistics a Biometrical Approach. McGraw-Hill Company, New York
Wang, PJ & Hu, CY (1985) Potato tissue culture and its applications in agriculture. In: Li, PH (Ed) Potato Physiology (pp 503–577). Academic Press, New York
Wattimena, GA (1983) Micropropagation as an alternative technology for potato production in Indonesia. Ph.D. Thesis. University of Wisconsin-Madison Microfilms International, Ann Arbour, Michigan. 202 pp
Wiersema, SG, Cabello, R, Tovar, P & Dodds, JH (1987) Rapid seed multiplication by planting into beds microtubers and in vitro plants. Potato Res. 30: 117–120
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Leclerc, Y., Donnelly, D.J. & Seabrook, J.E.A. Microtuberization of layered shoots and nodal cuttings of potato: The influence of growth regulators and incubation periods. Plant Cell Tiss Organ Cult 37, 113–120 (1994). https://doi.org/10.1007/BF00043604
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00043604