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Induction of Haploid Embryos in Datura metel by Anther Culture

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2288))

Abstract

Microspores, with a haploid number of chromosomes, are destined to produce the male gametophyte, which hosts the male gametes that fertilize the female egg cell. During microsporogenesis, a particular stage of development is amenable to be switched to undergo embryogenesis and developed into a haploid plant. By doubling the chromosomes, a doubled haploid plant, homozygous for all the gene loci, is produced. These plants are useful to study the expression of recessive genes and in plant breeding as a rapid pathway to achieve homozygosity.

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Correspondence to Mohammed C. M. Iqbal .

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Wijesekara, K.B., Iqbal, M.C.M. (2021). Induction of Haploid Embryos in Datura metel by Anther Culture. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1335-1_21

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  • DOI: https://doi.org/10.1007/978-1-0716-1335-1_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1334-4

  • Online ISBN: 978-1-0716-1335-1

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