Abstract
The addition of l-arginine, casein hydrolysate, banana powder and the reduction of the concentration of sucrose in the media could increase the solasodine content in the shoot cultures of Solanum laciniatum. No linear correlations between growth index, chlorophyll content and solasodine content were observed, however a positive linear correlation between solasodine productivity and chlorophyll content occurred in these shoot cultures.
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Abbreviations
- Ch:
-
chlorophyll content
- DW:
-
dry weight
- fl:
-
flask
- FW:
-
fresh weight
- GI:
-
growth index
- LOD:
-
limit of detection
- LOQ:
-
limit of quantitation
- SDc:
-
solasodine content
- SDp:
-
solasodine productivity
- w:
-
week
References
Atipayakul S & Jatisatienr A (1989) Induction of solasodine and callus development in tissue cultures of Solanum laciniatum, Poster presented to the 37th Annual Congress on Medicinal Plant Research, Braunschweig
Carle R (1979) Untersuchungen zur Steroidalkaoid und Sapogen-inführung in Pflanzen und Zellkulturen der Gatung Solanum L. Ph.D thesis, University of Tübingen
Chandler S F & Dodds J H (1983a) The effect of phosphate, nitrogen and sucrose on the production of phenolic and solasodine in callus cultures of Solanum laciniatum, Plant Cell Rep 2: 205–208
Chandler S F & Dodds J H (1983b) Solasodine production in rapidly proliferating tissue cultures of Solanum laciniatum Ait. Plant Cell Rep. 2: 69–72
Conner A J (1987) Differential solasodine accumulation in photoautotrophic and heterotrophic tissue cultures of Solanum laciniatum. Phytochemistry 26: 2749–2750
Ehmke A & Eilert U (1986) Steroidal alkaloid in tissue cultures and regenerated plants of Solanum dulcamara, Plant Cell Rep. 5: 31–34
Ehmke A & Eilert U (1993) Solanum duleamara L.: Accumulation of steroidal alkaloids in the plant and different in vitro systems. In: Bajaj Y P S (ed) Biotechnology in Agriculture and Forestry. Vol 21 (pp 346–347), Springer Verlag, Berlin Heidelberg
Handayani R (1990) Percobaan induksi pembentukan solasodina pada kultur kalus Solanum wrightii Benth. Skripsi, Fakultas Farmasi, Universitas Airlangga, Surabaya
Harborne J B (1973) Phytochemical Methods (pp 205–207). Chapman and Hall, London, Toppan Company, Limited, Tokyo, Japan
Hartojo W (1986) Pemakaian buah pisang masak pada media untuk kultur jaringan Solanum mammosum L. dan Solanum tuberosum L. Skripsi, Fakultas Farmasi, Universitas Airlangga, Surabaya
Heble M R, Narayanaswamy S & Chadia M S (1976) Metabolism of cholesterol by callus cultures of Holarrhena antidy-sentrica. Phytochemistry 15: 1911–1912
Indrayanto G (1983) Steroide und Triterpene in Zellkulturen, Ph.D thesis. University of Tuebingen
Kaneko K, Tanaka M W & Mitsuhashi (1976) Origin of nitrogen in the biosynthesis of solanidine by Veratrum grandiflorum, Phytochemistry 15: 1391–1993
Macek T E (1989) Solanum aviculare Forst., Solanum laciniatum Ait. (Poroporo): In vitro culture and the production of solasodine. In: Bajaj Y P S (ed) Biotechnology in Agriculture and Forestry, Vol 7 (pp 443–463), Springer Verlag, Berlin Heidelberg
Mak Y & Doran P M (1993) Effect of cell-cycle inhibition on synthesis of steroidal alkaloids by Solanum aviculare plant cells. Biotech. Lett. 15: 1031–1034
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nigra H M, Caso O H & Giulietti A M (1987) Production of solasodine by calli from different parts of Solanum ealeagnifolium Cav. plants. Plant Cell Rep. 6: 135–137
Nigra H M, Alvarez M A & Giulietti A M (1990) Effect of carbon and nitrogen sources on growth and solasodine production in batch suspension cultures of Solanum eleagnifolium Cav. Plant Cell Tiss. Org. Cult. 21: 55–60
Pierik R I M (1987) In vitro Cultures of Higher Plants (pp 157–158). Martinus Nijhof Publishers, Dordrecht, Boston, Lancaster
Schripsema J (1991) Factors involved in the alkaloid production of Tabernaemontana divaricata plant cell suspension cultures. Ph.D thesis, University of Leiden
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Indrayanto, G., Erawati, T. & Santosa, M.H. Effect of l-arginine, casein hydrolysate, banana powder and sucrose on growth and solasodine production in shoot cultures of Solanum laciniatum . Plant Cell Tiss Organ Cult 43, 237–240 (1995). https://doi.org/10.1007/BF00039950
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DOI: https://doi.org/10.1007/BF00039950