Abstract
Somatic embryo formation from immature cotyledons was improved in the following ways: by cutting into sections, supplementing culture media with spermine and using solid/liquid/solid type of culture. Cut cotyledons of the eight genotypes examined expressed a higher ability for somatic embryogenesis than whole cotyledons. Of the three polyamines tested, spermine considerably stimulated and putrescine slightly inhibited induction of somatic embryos. The ability of embryoid formation on medium with spermidine depended on the genotype. The solid/liquid/solid type of culture was better than the continuous solid culture. The best nitrogen ion content for the subculture of somatic embryos was 10 mM NH4NO3 and 30 mM KNO3. The possibility of using these modifications in Agrobacterium transformation is discussed.
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References
Buchheim, J.A., S.M., Colburn & J.P., Ranch, 1989. Maturation of soybean somatic embryos and the transition to plantlet growth. Plant Physiol. 89: 769–775.
Christou, P. & N-S., Yang, 1989. Developmental aspects of soybean (Glycine max) somatic embryogenesis. Ann. Bot. 64: 225–234.
Dunwell, J.M., R.J., Francis & W., Powell, 1987. Anther culture of Hordeum vulgare L.: a genetic study of microspore callus production and differentiation. Theor. Appl. Genet. 74: 60–64.
Graybosch, R.A., M.E., Edge & X., Delannay, 1987. Somaclonal variation in soybean plants regenerated from cotyledonary node tissue culture system. Crop Sci. 27: 803–806.
Hammatt, N. & M.R., Davey, 1987. Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean (Glycine max L. Merr.). J. Plant Physiol. 128: 219–226.
Hartweck, L.M., P.A., Lazzeri, D., Cui, G.B., Collins & E.G., Williams, 1988. Auxin-orientation effects on somatic embryogenesis from Immature soybean cotyledons. In Vitro Cell Developmental Biology 24: 821–828.
Komatsuda, T., K., Kaneko & S., Oka, 1991. Genotype x sucrose interactions for somatic embryogenesis in soybean. Crop Sci. 31: 333–337.
Komatsuda, T., W., Lee & S., Oka, 1992. Maturation and germination of somatic embryos as affected by sucrose and plant growth regulators in soybeans Glycine gracilis Skvortz and Glycine max (L.) Merr. Plant Cell, Tissue and Organ Culture 28: 103–113.
Komatsuda, T. & K., Ohyama, 1988. Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max. Theor. Appl. Genet. 75: 695–700.
Lazzeri, P.A., D.F., Hildebrand & G.B., Collins, 1987a. Soybean somatic embryogenesis: Effects of hormones and culture manipulations. Plant Cell, Tissue and Organ Culture 10: 197–208.
Lazzeri, P.A., D.F., Hildebrand & G.B., Collins, 1987b. Soybean somatic embryogenesis: Effects of nutritional, physical and chemical factors. Plant Cell, Tissue and Organ Culture 10: 209–220.
Lazzeri, P.A., D.F., Hildebrand, J., Sunega, E.G., Williams & G.B., Collins, 1988. Soybean somatic embryogenesis: interactions between sucrose and auxin. Plant Cell Report 7: 517–520.
Mengoli, M. & N., Bagni, 1992. Polyamines and somatic embryogenesis in higher plants. Newsletter IAPTC 68: 2–8.
Nadolska-Orczyk, A. & S., Malepszy, 1989. In vitro culture of Cucumis sativus L. & Genes controlling plant regeneration. Theor. Appl. Genet. 78: 836–840.
Nadolska-Orczyk, A., 1992. Somatic embryogenesis of agriculturally important lupin species (Lupinus angustifolius, L. albus, L. mutabilis). Plant Cell, Tissue and Organ Culture 28: 19–25.
Nadolski-Orczyk, A., L. Milowska & W. Orczyk, 1994. Two ways of plant regeneration from immature cotyledons of pea. Acta Societatis Botanicorum Poloniae 63(2).
Parrott, W.A., E.G., Williams, D.F., Hildebrand & G.B., Colins, 1989. Effect of genotype on somatic embryogenesis from immature cotyledons of soybean. Plant Cell, Tissue and Organ Culture 16: 15–21.
Ranch, J.P., L., Oglesby & A.C., Zielinski, 1985. Plant regeneration from embryo-derived tissue cultures of soybeans. In Vitro Cellular and Developmental Biology 21: 653–658.
Wright, M.S., D.V., Ward, M.A., Hinchee, M.G., Carnes & R.J., Kaufman, 1987. Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue. Plant Cell Report 6: 83–89.
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Nadolska-Orczyk, A., Orczyk, W. New aspects of soybean somatic embryogenesis. Euphytica 80, 137–143 (1994). https://doi.org/10.1007/BF00039309
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DOI: https://doi.org/10.1007/BF00039309