Abstract
The isolation and characterization is described of sporamin A and B genes, gSPO-A1 and gSPO-B1, respectively, which are members of two distinct subfamilies of the multigene family encoding the major soluble protein of sweet potato tuberous root. The nucleotide sequences of the two genes along with the 5′ and 3′ flanking regions were determined. The transcriptional start sites were determined by S1 nuclease mapping. Comparison of the sequences with those of the full-length cDNAs for sporamin A and B reveals that these genes contain no introns. Sequence comparison by dot matrix plot analysis shows that homology of the 5′ flanking regions between gSPO-A1 and gSPO-B1 extends only to 45 bp upstream of the transcription start site, which includes the consensus TATA box sequence. The sequence of this region is also conserved in the sporamin-related gene, gSPO-X1, which dose not seem to be expressed in the tuberous root. The sequences further upstream diverge extensively, but two short sequence blocks of 28 to 29 bp and 19 to 22 bp are found in the 5′ flanking sequence of gSPO-A1 and gSPO-B1 but not in gSPO-X1. The 19–22 bp sequence block is repeated twice in gSPO-A1 and four times in gSPO-B1. These conserved sequence blocks may play regulatory roles in their expression.
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Baumann G, Raschke E, Bevan M, Schoeffl F: Functional analysis of sequences required for transcriptional activation of a soybean heat shock gene in transgenic tobacco plants. EMBO J 6: 1161–1166 (1987).
Bevan M, Barker R, Goldsbrough A, Jarvis M, Kavanagh T, Iturriaga G: The structure and transcription start site of a major potato tuber protein gene. Nucleic Acids Res 14: 4625–4631 (1986).
Chen Z-L, Schuler MA, Beachy RN: Functional analysis of regulatory elements in a plant embryo specific gene. Proc Natl Acad Sci USA 83: 8560–8564 (1986).
Dynan WS, Tjian R: Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding protein. Nature 316: 774–778 (1985).
Fischer RL and Goldberg RB: Structure and flanking regions of soybean seed protein genes. Cell 29: 651–660 (1982).
Forde BG, Heyworth A, Pywell J, Kreis M: Nucleotide sequence of a B1 hordein gene and the identification of possible regulatory elements in endosperm storage protein genes from barley, wheat and maize. Nucleic Acids Res 13: 7327–7339 (1985).
Frischauf A-M, Lehrach H, Poustka A, Murray N: Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170: 827–842 (1983).
Halling KC, Halling AC, Murray EE, Ladin BF, Houston LL, Weaver RF: Genomic cloning and characterization of a ricin gene from Ricinus communis Nucleic Acids Res 13: 8019–8033 (1985).
Hammond RW, Ford DE, Larkins BA: Molecular cloning and analysis of a gene coding for the Bowman-Birk protease inhibitor. J Biol Chem 259: 9883–9890 (1984).
Hattori T, Nakagawa T, Maeshima M, Nakamura K, Asahi T: Molecular cloning and nucleotide sequence of cDNA for sporamin, the major soluble protein of sweet potato tuberous roots. Plant Mol Biol 5: 313–320 (1985).
Hearing P, Shenk T: Adenovirus enhancer elements. In: Gluzman Y (ed) Eukaryotic Transcription, pp 48–52, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1985).
Heidecker G, Messing J: Structural analysis of plant genes. Ann Rev Plant Physiol 37: 439–466 (1986).
Huynh TV, Young RA, Davis RW: Constructing and screening cDNA libraries in λgt10 and λgt11. In: Glover DM (ed) DNA Cloning, Vol 1, pp 49–78. IRL Press, Oxford (1985).
Kuhlemeier C, Green PJ, Chua N-H: Regulation of gene expression in higher plants. Ann Rev Plant Physiol 38: 221–257 (1987).
Maeshima M, Sasaki T, Asahi T: Characterization of major proteins in sweet potato tuberous roots. Phytochemistry 24: 1899–1902 (1985).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Messing J: New M13 vectors for cloning. Methods Enzymol 101: 20–78 (1983).
Mignery GA, Pikaard CS, Hannapel DJ, Park WD: Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin. Nucleic Acids Res 12: 7987–8000 (1984).
Murakami S, Hattori T, Nakamura K: Structural differences in full-length cDNAs for two classes of sporamin, the major soluble protein of sweet potato tuberous roots. Plant Mol Biol 7: 343–355 (1986).
Park WD: Tuber proteins of potato-A new and surprising molecular system. Plant Mol Biol Reporter 1: 61–66 (1983).
Pikaard CS, Mignery GA, Ma DP, Stark VJ, Park WD: Seauence of two apparent pseudogenes of the major potato protein, patatin. Nucleic Acids Res 14: 5564–5566 (1986).
Rafalski JA, Scheets K, Metzler M, Peterson DM, Hedgcoth C, Sooll DG: Developmentally regulated plants genes: the nucleotide sequence of a wheat gliadin genomic clone. EMBO J 3: 1409–1415 (1984).
Rosahl S, Eckes P, Schell J, Willmitzer L: Organ-specific gene expression in potato: isolation and characterization of tuber-specific cDNA sequences. Mol Gen Genet 202: 368–373 (1986).
Rosahl S, Schmidt R, Schell J and Willmitzer L: Isolation and characterization of a gene from Solanum tuberosum encoding patatin, the major storage protein of potato tubers. Mol Gen Genet 203: 214–220 (1986).
Vieira J and Messing J: Production of single stranded plasmid DNA. Methods Enzymol, in press (1987).
Vodkin LO, Rhodes PR, Goldberg RB: cA lectin gene insertion has the structural features of a transposable element. Cell 34: 1023–1031 (1983).
Yanisch-Perron C, Vieira J, Messing J: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103–119 (1985).
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Hattori, T., Nakamura, K. Genes coding for the major tuberous root protein of sweet potato: Identification of putative regulatory sequence in the 5′ upstream region. Plant Mol Biol 11, 417–426 (1988). https://doi.org/10.1007/BF00039022
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DOI: https://doi.org/10.1007/BF00039022