Abstract
Twenty clones established from single cells of a suspension culture of Capsicum frutescens were maintained as callus and in suspension over a sixteen week culture period. These clones exhibited marked differences in growth, chlorophyll and chloroform-soluble phenolic content which became more apparent with increasing time in culture. Clones in suspension exhibited a more rapid change in morphology and biosynthetic activity than those cultured as callus. Elicitation increased PAL activity, reduced the incorporation of L-[U-14C] phenylalanine into the chloroform-soluble fraction of the culture medium and increased incorporation into the methanol-soluble fraction of the cells in ten suspension clones. Differences to elicitation were observed among clones; in particular the faster growing isolates incorporated more radioactive label into soluble phenolics that remain in the cells than those that are released into the medium. The implications of these results are discussed.
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Abbreviations
- SH:
-
Schenk & Hildebrandt
- PAL:
-
phenylalanine ammonia-lyase
- RGR:
-
relative growth rate
- TCC:
-
total chlorophyll content
- HPLC:
-
high performance liquid chromatography
References
Bailey JA & Mansfield JW (1982) Phytoalexins. Blackie, Glasgow
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye-binding. Anal. Biochem. 72: 248–254
Creche J, Guiller J, Andreu F, Gras M, Chenieux JC & Rideau M (1987) Variability in tissue cultures of Choisya ternata originating from a single tree. Phytochemistry. 26: 1947–1953
Davey MR, Fowler MW & Street HE (1971) Cell clones contasted in growth, morphology and pigmentation isolated from a callus culture of Atropa belladona var. lutea. Phytochemistry. 10: 2559–2575
Dix PJ (1990) Plant Cell Line Selection: Procedures and Applications. VCH Weinhein, New York: Basel: Cambridge
Dixon RA & Harrison MJ (1990) Activation, structure and organisation of genes involved in microbial defense in plants. Adv. Genet. 28: 165–234
Dougall DK & Vogelien DL (1990) Anthocyanin yield of clonal wild carrot cultures. Plant Cell Tiss. Org. Cult. 23: 79–91
Dougall DK, Johnson JM & Whitten GH (1980) A clonal analysis of anthocyanin accumulation by cell cultures of wild carrot. Planta 149: 292–297
Hahlbrock K, Lamb CL, Purwin C, Ebel J, Fautz E & Schafer E (1981) Rapid response of suspension-cultured parsley cells to elicitors from Phytophthora megasperma var. soyae. Plant Physiol. 67: 768–777
Hall RD & Yeoman MM (1991) The influence of intracellular pools of phenylalanine derivatives upon the synthesis of capsaicin by immobilized cell cultures of the chilli pepper, Capsicum frutescens. Planta 185: 72–80
Hall RD, Holden MA & Yeoman MM (1987) The accumulation of phenylpropanoid and capsaicinoid compounds in cell cultures and whole fruit of the chilli pepper, Capsicum frutescens Mill. Plant Cell Tiss. Org. Cult. 8: 163–176
Harborne JB (1976) Phytochemical methods: A guide to modern techniques in plant analysis. Chapman and Hall, London
Holden MA, Hall RD, Lindsey K & Yeoman MM (1987) Capsaicin biosynthesis in cell cultures of Capsicum frutescens. In: Webb C & Mavituna F (Eds) Plant and Animal Cells, Process Possibilities (pp 45–63). Ellis Horwood Books in Biochemistry & Biotechnology, Ellis Horwood Limited, Chichester
Lindsey K & Yeoman MM (1984) The synthetic potential of immobilized cells of Capsicum frutescens Mill. cv. annuum. Planta 162: 495–501
Mantell SH & Smith H (1983) Cultural factors that influence secondary metabolite accumulations in plant cell and tissue cultures. In: Mantell SH & Smith H (Eds) Plant Biotechnology (pp 75–110). Cambridge University Press, Cambridge
Mavituna F, Park JM, Wilkinson AK & Williams PD (1987) Characteristics of immobilized plant cell reactors. In: Webb C & Mavituna F (Eds) Plant and Animal Cells, Process Possibilities (pp 92–115). Ellis Horwood Books in Biochemistry & Biotechnology, Ellis Horwood Limited, Chichester
Ozeki Y & Komamine A (1985) Changes in activities of enzymes involved in general phenylpropanoid metabolism during the induction and reduction of anthocyanin synthesis in a carrot suspension culture as regulated by 2,4-D. Plant Cell Physiol. 26: 903–911
Salgado-Garciglia R & Ochoa-Alejo N (1990) Increased capsaicin content in PFP-resistant cells of the chilli pepper (Capsicum annuum). Plant Cell Rep. 8: 617–620
Schenk RU & Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204
Selby C & Collin HA (1976) Clonal variation in growth and flavour production in tissue cultures of Allium cepa L. Ann. Bot. 40: 911–918
Sucrasno N & Yeoman MM (1993) Phenylpropanoid metabolism during growth and development of Capsicum frutescens fruits. Phytochemistry. 32: 839–844
Tanaka Y, Kojima M & Uritani I (1974) Properties, development and cellular localisation of cinnamic acid 4-hydroxylase in cut injured sweet potato. Plant Cell Physiol. 15: 843–854
Yeoman MM (1987) Bypassing the plant. Ann. Bot. 60: 157–174
Yeoman MM & Forche E (1980) Cell proliferation and growth in callus cultures. Int. Rev. Cytol. Suppl. 11A: 1–24
Zenk MH, El-Shagi H, Arens H, Stockigt J, Weiler EW & Deus B (1977) Formation of the indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In: Barz W, Reinhard E & Zenk MH (Eds) Plant Tissue Culture and its Biotechnological Application (pp 27–43). Springer-Verlag, Berlin and New York
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Holden, P.R., Yeoman, M.M. Variation in the growth and biosynthetic activity of cloned cell cultures of Capsicum frutescens and their response to an exogenously supplied elicitor. Plant Cell Tiss Organ Cult 38, 31–37 (1994). https://doi.org/10.1007/BF00034440
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DOI: https://doi.org/10.1007/BF00034440