Abstract
The quantitative importance of photosynthetically produced dissolved organic carbon (PDOC) released from phytoplankton as a source of carbon for pelagic, heterotrophic bacteria was investigated in four temperate Swedish lakes, of which two had low (≈20 mg Pt 1−1), and two moderately high (60–80 mg Pt 1−1) humic content. The bacterial assimilation of PDOC was estimated with the 14C method, and the total production of the heterotrophic bacteria was estimated with the [3H]thymidine incorporation method. The release of PDOC from natural communities of phytoplankton was not restricted to periods of photosynthesis, but often continued during periods of darkness. Heterotrophic bacteria often assimilated the labile components of the PDOC at high rates (up to 73% of the released PDOC was assimilated during the incubation in our experiments). The contribution of PDOC to bacterial production exhibited large within-lake seasonal variations, but PDOC was at certain times, both in humic and non-humic lakes, a quantitatively very important carbon source for the heterotrophic bacteria. Under periods of comparatively low primary production, heterotrophic bacteria in humic lakes appear to utilize allochthonous, humic substances as a substrate.
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Bell, R. T., 1986. Further verification of the isotope dilution approach for estimating the degree of participation of [3H]thymidine in studies of aquatic bacterial production. Appl. envir. Microbiol. 52: 1212–1214.
Bell, R. T., 1990. An explanation for the variability in the conversion factor deriving bacterial cell production from incorporation of [3H]thymidine. Limnol. Oceanogr. 35: 910–915.
Bell, R. T. & J. Kuparinen, 1984. Assessing phytoplankton and bacterioplankton production during early spring in Lake Erken, Sweden. Appl. envir. Microbiol. 48: 1221–1230.
Bell, R. T. & B. Riemann, 1989. Adenine incorporation into DNA as a measure of microbial production in freshwaters. Limnol. Oceanogr. 34: 435–444.
Bell, R. T., G. M. Ahlgren & I. Ahlgren, 1983. Estimating bacterioplankton production by measuring [3H]thymidine incorporation in a eutrophic Swedish lake. Appl. envir. Microbiol. 45: 1709–1721.
Berman, T. & B. Kaplan, 1984. Diffusion chamber studies of carbon flux from living algae to heterotrophic bacteria. Hydrobiologia 108: 127–134.
Bern, L., 1985. Autoradiographic studies of [methyl-3 H]thymidine incorporation in a cyanobacterium (Microcystis wesenbergii)-bacterium association and in selected algae and bacteria. Appl. envir. Microbiol. 49: 232–233.
Bird, D. F. & J. Kalff, 1984. Empirical relationships between bacterial abundance and chlorophyll concentration in fresh and marine waters. Can. J. Fish. aquat. Sci. 41: 1015–1023.
Bjornsen, P. K., 1988. Phytoplankton exudation of organic matter: Why do healthy cells do it? Limnol. Oceanogr. 33: 151–154.
Bjornsen, P. K. & B. Riemann, 1988. Towards a quantitative stage in the study of microbial processes in pelagic carbon flows. Arch. Hydrobiol. Beih. Ergebn. Limnol. 31: 185–193.
Bostrom, B., A.-K. Pettersson & I. Ahlgren, 1989. Seasonal dynamics of a cyanobacteria dominated microbial community in surface sediments of a shallow, eutrophic lake. Aquat. Sci. 51: 153–178.
Bratbak, G. & I. Dundas, 1984. Bacterial dry matter content and biomass estimations. Appl. envir. Microbiol. 48: 755–757.
Bratbak, G. & T. F. Thingstad, 1985. Phytoplankton-bacteria interactions: an apparent paradox? Analysis of a model system with both competition and comensalism. Mar. Ecol. Prog. Ser. 25: 23–30.
Brock, T. D. & J. Clyne, 1984. Significance of algal excretory products for growth of epilinmetic bacteria. Appl. envir. Microbiol. 47: 731–734.
Chin-Leo, G. & D. L. Kirchman, 1988. Estimating bacterial production in marine waters from the simultaneous incorporation of thymidine and leucine. Appl. envir. Microbiol. 54: 1394–1399.
Chróst, R. J., 1983. Plankton photosynthesis, extracellular release, and bacterial utilization of released dissolved organic carbon (RDOC) in lakes of different trophy. Acta Microbiol. Polon. 32: 275–287.
Chróst, R. J. & M. A. Faust, 1983. Organic carbon release by phytoplankton: its composition and utilization by bacterioplankton. J. Plankton Res. 5: 477–493.
Chrzanowski, T. H. & J. G. Hubbard, 1989. Bacterial utilization of algal extracellular products in a southwestern reservoir. Hydrobiologia 179: 61–71.
Cole, J. J., S. Findlay & M. L. Pace, 1988. Bacterial production in fresh and saltwater ecosystems: A cross-system overview. Mar. Ecol. Prog. Ser. 42: 1–10.
Cole, J. J., G. E. Likens & D. L. Strayer, 1982. Photosynthetically produced dissolved organic carbon: an important carbon source for planktonic bacteria. Limnol. Oceanogr. 27: 1080–1090.
Cole, J. J., W. H. McDowell & G. E. Likens, 1984. Sources and molecular weight of `dissolved' organic carbon in an oligotrophic lake. Oikos 42: 1–9.
Coveney, M. F., 1982. Bacterial uptake of photosynthetic carbon from freshwater phytoplankton. Oikos 38: 8–20.
Coveney, M. F. & R. G. Wetzel, 1989. Bacterial metabolism of algal extracellular carbon. Hydrobiologia 173: 141–149.
De Haan, H., 1977. Effect of benzoate on microbial decomposition of fulvic acids in Tjeukemeer (the Netherlands). Limnol. Oceanogr. 22: 38–44.
Dunstall, T. G. & C. Nalewajko, 1975. Extracellular release in planktonic bacteria. Verb. Int. Ver. Limnol. 19: 2643–2649.
Feuillade, M., P. Dufour & J. Feuillade, 1988. Organic carbon release by phytoplankton and bacterial reassimilation. Schweiz. Z. Hydrol. 50: 115–135.
Goldman, J. C. & M. R. Dennet, 1985. Susceptibility of some marine phytoplankton species to cell breakage during filtration and post-filtration rinsing. J. exp. mar. Biol. Ecol. 86: 47–58.
Hellebust, J. A., 1965. Excretion of some organic compounds by marine phytoplankton. Limnol. Oceanogr. 10: 192–206.
Hessen, D. O., 1985. The relation between bacterial carbon and dissolved humic compounds in oligotrophic lakes. FEMS Microbiol. Ecol. 31: 215–223.
Heyman, U. & P. Blomqvist, 1984. Diurnal variations in phytoplankton cell numbers and primary productivity in Siggeforasjon. Arch. Hydrobiol. 100: 219–233.
Hobbie, J. E. & J. J. Cole, 1984. Response of a detrital foodweb to eutrophication. Bull. mar. Sci. 35: 357–363.
Iturriaga, R. & A. Zsolnay, 1981. Transformation of some dissolved organic compounds by a natural heterotrophic population. Mar. Biol. 62: 125–129.
Jassby, A. D. & C. R. Goldmann, 1974. Loss rates from a lake phytoplankton community. Limnol. Oceanogr. 19: 618–627.
Jensen, L. M., 1983. Phytoplankton release of extracellular organic carbon, molecular weight composition, and bacterial assimilation. Mar. Ecol. Prog. Ser. 11: 39–48.
Jensen, L. M., N. O. G. Jörgensen & M. Söndergaard, 1985. Specific activity. Significance in estimating release rates of extracellular dissolved organic carbon (EOC) by algae. Verb. int. Ver. Limnol. 22: 2893–2897.
Jones, R. I. & K. Salonen, 1985. The importance of bacterial utilization of released phytoplankton photosynthate in two humic forest lakes in southern Finland. Holarct. Ecol. 8: 133–140.
Jumars, P. A., D. L. Penry, J. A. Baross, M. J. Perry & B. W. Frost, 1989. Closing the microbial loop: dissolved carbon pathway to heterotrophic bacteria from incomplete ingestion, digestion and absorption in animals. Deep Sea Res. 36: 483–495.
Jürgens, K. & H. Güde, 1990. Incorporation and release of phosphorus by planktonic bacteria and phagotrophic flagellates. Mar. Ecol. Prog. Ser. 59: 271–284.
Kuosa, H., 1988. Enumeration of autotrophic and heterotrophic flagellates in Baltic Sea samples — a comparison of microscopical methods. Arch. Hydrobiol. Beih. Ergebn. Limnol. 31: 301–306.
Lampert, W., 1978. Release of dissolved organic carbon by grazing zooplankton. Limnol. Oceanogr. 23: 831–834.
Larsson, U. & Å. Hagström, 1982. Fractionated phytoplankton primary production, exudate release and bacterial production in a Baltic eutrophication gradient. Mar. Biol. 67: 57–70.
Olsen, Y., K. M. V»rum & A. Jensen, 1986. Some characteristics of the carbon compounds released by Daphnia. J. Plankton Res. 8: 505–517.
Pearl, H. W., 1982. Interactions with bacteria. In N. G. Carr & B. A. Whitton (eds), The Biology of Cyanobacteria. University of California Press, Berkley & Los Angeles: 441–461.
Pettersson, K., 1990. The spring development of phytoplankton in Lake Erken: species composition, biomass, primary production and nutrient conditions — a review. Hydrobiologia 191: 9–14.
Pollinger, U. & C. Serruya, 1976. Phased division of Peridinium cinctum f. Westii (Dinophyceae) and development of the Lake Kinneret (Israel) bloom. J. Phycol. 12: 162–170.
Riemann, B. & R. T. Bell, 1990. Advances in estimating bacterial biomass and growth in aquatic systems. Arch. Hydrobiol. 118: 385–402.
Riemann, B. & M. Söndergaard, 1984. Measurements of diel rates of bacterial secondary production in aquatic environments. Appl. envir. Microbiol. 47: 632–638.
Riemann, B., R. T. Bell & N. O. G. Jørgensen, 1990. Incorporation of thymidine, adenine, and leucine into natural bacterial communities. Mar. Ecol. Prog. Ser. 65: 87–94.
Saunders, G. W., 1972. The kinetics of extracellular release of soluble organic matter by plankton. Verh. int. Ver. Limnol. 18: 140–146.
Scavia, D. & G. A. Laird, 1987. Bacterioplankton in Lake Michigan: Dynamics, controls and significance to carbon flux. Limnol. Oceanogr. 32: 1017–1033.
Schwaerter, S., M. Søndergaard, B. Riemann & L. M. Jensen, 1988. Respiration in eutrophic lakes: the contribution of bacterioplankton and bacterial growth yield. J. Plankton Res. 10: 515–531.
Sederholm, H., A. Mauranen & L. Montonen, 1973. Some observations on the microbial degradation of humous substances in water. Verh. int. Ver. Limnol. 18: 1301–1305.
Simon, M. & F. Azam, 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Mar. Ecol. Prog. Ser. 51: 201–213.
Smith, R. E. H., 1982. The estimation of phytoplankton production and excretion by carbon-14. Mar. Biol. Lett. 3: 325–334.
Smits, J. & B. Riemann, 1988. Cell production derived from [3H]thymidine incorporation using freshwater bacteria. Appl. envir. Microbiol. 54: 2213–2219.
Sundh, I., 1989. Characterization of phytoplankton extracellular products (PDOC) and their subsequent uptake by heterotrophic organisms in a mesotrophic forest lake. J. Plankton Res. 11: 463–486.
Søndergaard, M. & H.-H. Shierup, 1982. Dissolved organic carbon during a spring diatom bloom in lake Mosso, Denmark. Wat. Res. 16: 815–821.
Søndergaard, M., B. Riemann & N. O. G. Jørgensen, 1985. Extracellular organic carbon (EOC) released by phytoplankton and bacterial production. Oikos 45: 323–332.
Tranvik, L. J., 1989. Bacterioplankton growth, grazing mortality and quantitative relationship to primary production in a humic and a clearwater lake. J. Plankton Res. 11: 985–1000.
Vadstein, O. & Y. Olsen, 1989. Chemical composition and phosphate uptake kinetics of limnetic bacterial communities cultured in chemostats under phosphorus limitation. Limnol. Oceanogr. 34: 939–946.
Vadstein, O., B. O. Harkjerr, A. Jensen, Y. Olsen & H. Reinerstein, 1989. Cycling of organic carbon in the photic zone of a eutrophic lake with special reference to the heterotrophic bacteria. Limnol. Oceanogr. 34: 840–855.
Vadstein, O., A. Jensen, Y. Olsen & H. Reinerstein, 1988. Growth and phosphorus status of limnetic phytoplankton and bacteria. Limnol. Oceanogr. 33: 489–503.
Wetzel, R. G., 1983. Limnology (Second edition), Saunders College Publishing, 858 pp.
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Sundh, I., Bell, R.T. Extracellular dissolved organic carbon released from phytoplankton as a source of carbon for heterotrophic bacteria in lakes of different humic content. Hydrobiologia 229, 93–106 (1992). https://doi.org/10.1007/BF00006993
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DOI: https://doi.org/10.1007/BF00006993