Abstract
In horticulture, inhibition of shoot branching during orchards establishment is highly desired to avoid stem break by wind while increased branching during crop production usually leads to increased yields. In forestry, reduced branching significantly improves wood quality, especially for pulp production. Thus, branching control is an important component on the establishment of productivity rates for crop and forestry species. Brassinosteroids (BRs) are natural polyhydroxy steroidal lactones and ketones with phytohormone action. These plant steroids are known to stimulate stem elongation and to control apical dominance as well. Our research efforts have been focused on the use of BRs to control stem elongation and apical dominance aiming to improve crop and forestry species productivity, and yield in in vitro-based plant propagation techniques. In this chapter we describe how BRs can be used to significantly improve microprogation techniques, more especifically for the marubakaido apple rootstock.
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References
Abe, H., Morishita, T., Uchiyama, M., Takatsuto, S., Ikekawa, N., Ikeda, M., Sassa, T., Kitsuwa, T., Marumo, S. (1983). Occurrence of three new brassinosteroids: brassinone, (24S)-24-ethylbrassinone and 28-norbrassinolide in higher plants. Experientia 39: 351–353
Altmann, T. (1998). A tale of dwarfs and drugs: brassinosteroids to the rescue. Trends in Genetics 14: 490–495.
Arteca, R. N. (1995). Brassinosteroids. In Plant hormones: Physiology, biochemistry and molecular biology, pp. 206–213. Eds P J Davies. Kluwer Academic Publishers, Netherlands.
Arteca, R. N., Tsai, D. S., Mandava, N. B. (1991). The inhibition of brassinosteroid-induced ethylene biosynthesis in etiolated mung bean hypocotyl segments by 2,3,5-triiodobenzoic acid and 2-(pchlorophenoxy)-2-methylpropionic acid. J of Plant Physiology 139: 52–56
Azpiroz, R., Wu, Y., LoCascio, J. C., Feldmann, K. A. (1998). An Arabidopsis brassinosteroid-dependent mutant is blocked in cell elongation. Plant Cell 10: 219–230.
Back, T., Janzen, L., Nakajima, S., Pharis, R. (1999). Synthesis and biological activity of 25-methoxy-, 25- fluoro-, and 25 azabrassinolide and 25-fluorocastasterone: surprising effects of heteroatm substituent at C-25. Journal of Organic Chemistry 64: 5494–5498.
Bieberach, C., de León, B., Teme Centurión, O., Ramírez, J., Gros, E., Galagovsky, L. (2000). Estudios preliminares sobre el efecto de dos brassinosteroides sintéticos sobre el crecimiento in vitro de yuca, ñame y piña. Anales de la Asociación Quimica Argentina, 88:No1/2, 1–7.
Brosa, C. (1999). Structure-activity relationship. In Brassinosteroids: Steroidal Plant Hormones, pp. 191–222. Eds A Sakurai, T Yokota and S D Clouse. Springer Verlag, Tokyo.
Brosa, C., Capdevila, J. M., Zamora, I. (1996). Brassinosteroids: a new way to define the structural requirements. Tetrahedron 52: 2435–2448
Brosa, C., Nusimovich, S., Peracaula, R. (1994). Synthesis of new brassinosteroids with potential activity as antiecdysteroids. Steroids 59: 463–467
Brosa, C., Soca, L., Terricabras, E., Ferrer, J., Alsina, A. (1998). New synthetic brassinosteroids: a 5a- hydroxy-6-ketone analog with strong plant growth promoting activity. Tetrahedron 54: 12337–48.
Brosa, C., Zamora, I., Terricabras, E., Soca, L., Peracaula, R., Rodriguez-Santamarta, C. (1997). Synthesis and molecular modelling: related approaches to the progress in brassinosteroid research. Lipids 32: 1341–1347.
Choe, S., Dilkes, B.P., Gregory, B.D., Ross, A.S., Yuan, H., Noguchi, T., Fujioka, S., Takatsuto, S., Tanaka, A., Yoshida, S., Tax, F. E., Feldmann, K.A. (1999a). The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis. Plant Physiology 119: 897–907.
Choe, S., Noguchi, T., Fujioka, S., Takatsuto, S., Tissier, C.P., Gregory, B.D., Ross, A.S., Tanaka, A., Yoshida, S., Tax, F. E., Feldmann, K.A. (1999b). The Arabidopsis dwf7/ste1 mutant is defective in the Delta (7) sterol C-5 desaturation step leading to brassinosteroid biosynthesis. Plant Cell 11: 207–221.
Chon, N. M., Nishikawa-Koseki, N., Hirata, Y., Saka, H., Abe, H. (2000). Effects of brassinolide on mesocotyl, coleoptile and leaf growth in rice seedlings. Plant Production Science 3: 360–365.
Chory, J. (2001). Light, brassinosteroids, and Arabidopsis development. Proceedings of the Symposium: Plant Physiology 2000 and Beyond: Breaking the Mold, Plant Biology 2001-ASPP, Providence, Rhode Island, Abstract 30005.
Chory, J., Nagpal, P., Peto, C. A. (1991). Phenotypic and genetic analysis of det2, a new mutant that affects light-regulated seedling development in Arabidopsis. Plant Cell 3: 445–459.
Chory, J., Peto, C., Feinbaum, R., Pratt, L., Ausubel, F. (1989). Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light. Cell 58: 991–999.
Cleland, R. E (1995). In Plant hormones: Physiology, biochemistry and molecular biology, pp. 214–227. Eds P J Davies. Kluwer Academic Publishers, Netherlands.
Clouse, S.D. (1996). Molecular genetic studies confirm the role of brassinosteroids in plant growth and development. Plant Journal 10: 1–8.
Clouse, S.D. (2002). Brassinosteroid signal transduction: Clarifying the pathway from ligand perception to gene expression. Molecular Cell 10: 973–982.
Clouse, S. D., Sasse, J. M. (1998). Brassinosteroids: Essential regulators of plant growth and development. Annual Review of Plant Physiology and Plant Molecular Biology 49: 427–451.
Clouse, S. D., Zurek, D. (1991). Molecular analysis of brassinolide action in plant growth and development. In Brassinosteroids: Chemistry, Bioactivity, and Applications, pp. 122–140. Eds H G Cutler, T Yokota and G Adam. American Chemical Society, Washington.
Cosgrove, D. (1997). Relaxation in a high-stress environment: the molecular basis of extensible cell walls and enlargement. Plant Cell 9: 1031–1041.
Cutler, H. G., Yokota, T., Adam, G. (1991). Brassinosteroids: chemistry, bioactivity, and applications.pp. 358. American Chemical Society, Washington.
Dahse, I., Petzold, U., Willmer, C. M., Grimm, E. (1991). Brassinosteroid-induced changes of plasmalemma energization and transport and of assimilate uptake by plant-tissues. In Brassinosteroids: Chemistry, bioactivity, and applications, pp. 167–175. Eds H G Cutler, T Yokota and G Adam. American Chemical Society, Washington
Dunitz, J., Taylor, R. (1997). Organic fluorine hardly ever accepts hydrogen bonds. Chemistry- A European Journal. 3: 89–98.
Evans, M. L. (1985). The action of auxin on plant cell elongation. Critical Review of Plant Sciences. 2: 317–365.
Filler, R., Kobayashi, Y., Yagupolskii, L. (1993). Organofluorine Compounds in Medicinal Chemistry and Biomedical Applications. Elsevier, Amsterdam.
Flores, R., Lessa, A. O., Peters, J. A., Fortes, G. R. L. (1999). Efeito da sacarose e do benomyl na multiplicação in vitro da macieira. Pesquisa Agropecuaria Brasilleira., 34: 2363–2368.
Friedrichsen, D.M., Joazeiro, C. A. P., Li, J., Hunter, T., Chory, J. (2000). Brassinosteroid-insensitive-1 is a ubiquitously expressed leucine-rich repeat receptor serine/threonine kinase. Plant Physiology 123: 1247–1256.
Fujioka, S. (1999). Natural occurrence of brassinosteroids in the plant kingdom. In Brassinosteroids: Steroidal Plant Hormones, pp. 21–45. Eds A Sakurai, T Yokota and S D Clouse. Springer Verlag, Tokyo.
Fujioka, S., Sakurai, A. (1997). Biosynthesis and metabolism of brassinosteroids. Physiology Plantarum 100: 710–715.
Galagovsky, L., Gros, E., Ramírez, A. (2001). Synthesis and bioactivity of natural and C-3 fluorinated biosynthetic precursors of 28-homobrassinolide. Phytochemistry 58: 973–980.
Gaudinova, A., Sussenbekova, H., Vojtechova, M., Kaminek, M., Eder, J., Kohout, L. (1995). Different effects of 2 brassinosteroids on growth, auxin and cytokinin content in tobacco callus-tissue. Plant Growth Regulation 17: 121–126
Grove, M. D., Spencer, G. F., Rohwedder, W. K., Mandava, N., Worley, J. F., Warthen, J. D. Jr., Steffens, G. L., Flippen-Anderson, J. L., Cook, J. C. Jr. (1979). Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen. Nature 281: 216–217.
Howard, J., Hoy, V., O’Hagan, D., Smith, G. (1996). How good is fluorine as a hydrogen bond acceptor? Tetrahedron 38: 12613–12622.
Hu, Y., Bao, F., Li, J. (2000). Promotive effect of brassinosteroids on cell division involves a distinct CycD3-induction pathway in Arabidopsis. Plant Journal 24: 693–701.
Jin, F., Xu, Y., Huang, W. (1993). 2,2 Difluoro enol silyl ethers: convenient preparation and application to the synthesis of a novel fluorinated brassinosteroid. Journal of the Chemical Society, Perkin Transactions 1: 795–799.
Jiang, B., Ying, L., Zhou, W-S. (2000). Stereocontrolled synthesis of the 22E,240 (S) –trifluoromethyl steroidal side chain and its application to the synthesis of fluorinated analogues of naturally occurring sterols. Journal of Organic Chemistry 65: 2631–6236.
Joseph-Nathan, P., Espiñeira, J., Santillan, R. (1984). 19F-NMR study of fluorinated corticosteroids. Spectrochimica Acta 40A: 347–349.
Kamuro, Y., Takatsuto, S. (1999). Practical applications of brassinosteroids in agricultural fields. In: Brassinosteroids: Steroidal Plant Hormones. pp. 223–241. Eds A Sakurai, T Yokota and S D Clouse. Springer Verlag, Tokyo.
Kauschmann, A., Jessop, A., Koncz, C., Szekeres, M., Willmitzer, L., Altmann, T. (1996). Genetic evidence for an essential role of brassinosteroids in plant development. Plant Journal 9: 701–713.
Khripach, V. A., Zhabinskii, V. N., de Groot, A. E. (1999a). Bioassays and structure-activity relationships of BS. In Brassinosteroids: A New Class of Plant Hormones, pp 301–324. Eds V A Khripach, V N Zhabinskii and A E de Groot. Academic Press, San Diego.
Khripach, V. A., Zhabinskii, V. N., de Groot, A. E. (1999b). Physiological mode of action of BS. In Brassinosteroids: a New Class of Plant Hormones, pp 219–300. Eds V A Khripach, V N Zhabinskii, A E de Groot. Academic Press, San Diego.
Kim, G –T., Tsukaya, H., Uchimiya, H. (1998). The Rotundifolia 3 gene of Arabidopsis thaliana encodes a new member of the cytochrome P-450 family that is required for the regulated polar elongation of leaf cells. Genes and Development, 12: 2381–2391.
Kim, S., Abe, H., Little, C., Pharis, R. (1990). Identification of two brassinosteroids from the cambial region of Scots pine (Pinus silvestris) by gas-chromatography-mass spectrometry, after detection using a dwarf lamina inclination bioassay. Plant Physiology 94: 1709–1713.
Kishi, T., Wada, K., Marumo, S., Mori, K. (1986). Synthesis of brassinolide analogs with a modified ring B and their plant growth-promoting activity. Agricultural and Biological Chemistry 50: 1821–1830
Kobayashi, Y., Taguchi, T. (2000). Studies on organofluorine compounds: an overview of our 30 years. Yakugaky Zasshi 120: 951–958.
Kohout, L., Strand, M., Kaminek, M. (1991). Types of brassinosteroids and their bioassay. In Brassinosteroids: Chemistry, Bioactivity, and Applications, pp. 56–73. Eds H G Cutler, T Yokota and G Adam. American Chemical Society, Washington.
Koka, C. V., Cerny, R. E., Gardner, R. G., Noguchi, T., Fujioka, S., Takatsuto, S., Yoshida, S., Clouse, S. D. (2000). A putative role for the tomato genes DUMPY and CURL-3 in brassinosteroid biosynthesis and response. Plant Physiology 122: 85–98.
Ladyzhenskaya, E. P., Korableva, N. P. (2001). Effects of growth regulators on H+ translocation across the membranes of plasma membrane vesicles from potato tuber cells. Applied Biochemistry and Microbiology 37: 521–523.
Li, J., Chory, J. (1997). A putative leucine-rich receptor kinase involved in brassinosteroid signal transduction. Cell 90: 929–938.
Li, J., Chory, J. (1999). Brassinosteroid actions in plants. Journal of Experimental Botany 50: 275–282.
Li, J., Nagpal, P., Vitart, V., McMorris, T. C., Chory, J. (1996). A role for brassinosteroids in light-dependent development of Arabidopsis. Science 272: 398–401.
Li, J., Nam, K. H., Vafeados, D., Chory, D. (2001). BIN2, a new brassinosteroid-insensitive locus in Arabidopsis. Plant Physiology 127: 14–22.
Liebman, J., Greenberg, A., Dolbier, W. Jr., Eswarakrishnan, S. (1988). Fluorine-Containing Molecules: Structure, Reactivity, Synthesis. VCH Publisher, New York.
Maeda, E. (1965). Rate of lamina inclination in excised rice leaves. Physiology Plantarum 18: 813–827.
MacMorris, T., Chávez, R., Patil, P. (1996). Improved synthesis of brassinolide. Journal of the Chemical Society Perkin Transactions 1, 295–302.
Mandava, N. B. (1988). Plant growth-promoting brassinosteroids. Annual Review of Plant Physiology and Plant Molecular Biology 39: 23–52.
Martin, C., Galdwell, J., Graham, M., Grierson, J., Kroll, K., Cowan, M., Lwellen, T., Rasey, J. Casciari, J., Krohn, K. (1992). Non invasive detection of hypoxic myocardium using fluorine-18-fluoromisonidazole and positron emission tomography. Journal of Nuclear Medicine 22: 2202.
Mayumi, K., Shibaoka, H. (1995). A possible double role for brassinolide in the reorientation of cortical microtubules in the epidermal cells of Azuki bean epicotyls. Plant Cell Physiology 36: 173–181.
McMorris, T. C., Patil, P. A., Chavez, R. G., Baker, M. E., Clouse, S. D. (1994). Synthesis and biological activity of 28-homobrassinolide and analogues. Phytochemistry 36: 585–589.
Mori, K. (1980). Synthesis of a brassinolide analog with high plant growth promoting activity. Agricultural and Biological Chemistry 44: 1211–1212.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.
Nakayama, M., Yamane, H., Murofushi, N., Takahashi, N., Mander, L. N., Seto, H. (1991). Gibberellin biosynthetic pathway and the physiologically active gibberellin in the shoot of Cucumis sativus L. Journal of Plant Growth Regulation 10: 115–119.
Noguchi, T., Fujioka, S., Choe, S., Takatsuto, S., Tax, F. E., Yoshida, S., Feldmann, K. A. (2000). Biosynthetic pathways of brassinolide in Arabidopsis. Plant Physiology 124: 201–209.
Oh, M. H., Romanow, W., Smith, R., Zamski, E., Sasse, J., Clouse, S. (1998). Soybean BRU1 encodes a functional xyloglucan endo-transglycosylase that is highly expressed in inner epicotyl tissues during brassinosteroid-promoted elongation. Plant Cell Physiology 39: 124–130.
O’Hagan, D., Rzepa, H. (1997). Some influences of fluorine in bioorganic chemistry. Chemical Communications 645–652.
Okada, K., Mori, K. (1983). Stereoselective synthesis of dolicholide, a plant growth-promoting steroid. Agricultural Biological Chemistry 47: 925–926.
Ramírez, A., Gros, E., Galagovsky, L. (2000). Effect on bioactivity due to C-5 heteroatom substituents on synthetic 28-Homobrassinosteroids analogs. Tetrahedron 56: 6171–6181.
Richter, K., Koolman, J. (1991). Antiecdysteroid effects of brassinosteroids. In Brassinosteroids - Chemistry, Bioactivity and Applications, pp. 265–278. Eds H G Cutler, T Yokota and G Adam. American Chemical Society, Washington.
Saito, T., Kamiya, Y., Yamane, H., Murofushi, N., Sakurai, A., Takahashi, N. (1998). Effects of fluorogibberellins on plant growth and gibberellin 30-hydroxylases. Plant Cell Physiology 39: 574–580.
Sakurai, A. (1999). Biosynthesis. In Brassinosteroids: Steroidal Plant Hormones, pp. 91–111. Eds A Sakurai, T Yokota and S D Clouse. Springer Verlag, Tokyo.
Sasse, J. M. (1997). Recent progress in brassinosteroid research. Physiologia Plantarum 100: 696–701.
Schaefer, S., Medeiro, A. S., Ramirez, J. A., Galagovsky, L. R., Pereira-Netto, A. B. (2002). Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]. Plant Cell Reports 20: 1093–1097.
Schumacher, K., Vafeados, D., McCarthy, M., Sze, H., Wilkins, T., Chory, J. (1999). The Arabidopsis det3 mutant reveals a central role for the vacuolar H+-ATPase in plant growth and development. Genes and Development 13: 3259–3270.
Schwartzenberg, K., Doumas, P., Jouanin, L., Pilate, G. (1994). Enhancement of the endogenous cytokinin concentration in poplar by transformation with Agrobacterium T-DNA gene ipt. Tree Physiology 14: 27–35.
Seto, H., Fujioka, S., Koshino, H., Suenaga, T., Yoshida, S., Watanabe, T., Takatsuto, S. (1 998).Epimerization at C-5 of brassinolide with sodium methoxide and the biological activity of 5-epibrassinolide in the rice lamina inclination assay. Journal of the Chemical Society, Perkin Transactions 1: 3355–3358.
Seto, H., Fujioka, S., Koshino, H., Suenaga, T., Yoshida, S., Watanabe, T., Takatsuto, S. (1999). 2,3,5-Triepi-brassinolide: preparation and biological activity in rice lamina inclination test. Phytochemistry 52: 815–818.
Sharpless, K. B., Amberg, W., Bennani, Y. L. (1992). The osmium-catalyzed asymmetric dihydroxylation: a new ligand class and a process improvement. Journal of Organic Chemistry 57: 2768–2771.
Shekhawat, N. S., Rathore, T. S., Singh, R. P., Deora, N. S., Rao, S. R. (1993). Factors affecting in vitro clonal propagation of Prosopis cineraria. Plant Growth Regulation 12: 273–280.
Spray, C., Phinney, B. O., Gaskin, P., Gilmour, S. J., MacMillan, J. (1984). Internode length in Zea mays L. Planta1 60: 464–468
Szekeres, M., Nemeth, K., Koncz-Kalman, Z., Mathur, J., Kauschmann, A., Altmann, T., Redei, G. P., Nagy, F., Schell, J., Koncz, C. (1996). Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell 85: 171–182.
Takatsuto, S., Ikekawa, N., Morishita, T., Abe, H. (1987). Structure-activity relationship of brassinosteroids with respect to the A/B-ring functional groups. Chemical Pharmaceutical Bulletin. 35: 211–216.
Takatsuto, S., Yazawa, N., Ikekawa, N., Morishita, T., Abe, H. (1983a). Synthesis of (24R)-28- homobrassinolide and structure-activity relationships of brassinosteroids in the rice lamina inclination test. Phytochemistry 22: 1393–1397.
Takatsuto, S., Yazawa, N., Ikekawa, N., Takematsu, T., Takeuchi, Y., Koguchi, M. (1983b). Structure-activity relationship of brassinosteroids. Phytochemistry 22: 2437–2441.
Takeno, K., Pharis, R. (1982). Brassinosteroid-induced bending of the leaf lamina of dwarf rice seedlings: an auxin-mediated phenomenon. Plant Cell Physiology 23: 1275–1281.
Thompson, M. J., Mandava, N., Flippen-Anderson, J. L., Worley, J. F., Dutky, S. R., Robbins, W. E., Lusby, W. (1979). Synthesis of brassinosteroids: new plant-growth promoting steroids. Journal of Organic Chemistry 44: 5002–5004.
Thompson, M. J., Mandava, N. B., Meudt, W.J., Lusby, W. R., Spaulding, D. W. (1981). Synthesis and biological activity of brassinolide and its 220, 23 ß -isomer: novel plant growth promoting steroids. Steroids 38: 567–580.
Thompson, M. J., Meudt, W. J., Mandava, N. B., Dutky, S. R., Lusby, W. R., Spaulding, D. W. (1982). Synthesis of brassinosteroids and relationship of structure to plant growth-promoting effect. Steroids 39: 89–105.
Voigt, B., Takatsuto, S., Yokota, T., Adam, G. (1995). Synthesis of secasterone and further epimeric 2,3- epoxybrassinosteroids. Journal of the Chemical Society, Perkin Transactions 1: 1495–1498.
Wada, K., Marumo, S., Abe, H., Morishita, T., Nakamura, K., Uchiyama, M., Mori, K. (1984). A rice lamina inclination test–a micro-quantitative bioassay for brassinosteroids. Agricultural and Biological Chemistry 48: 719–726.
Wada, K., Marumo, S., Ikekawa, N., Morisaki, M., Mori, K. (1981). Brassinolide and homobrassinolide promotion of lamina inclination of rice seedlings. Plant and Cell Physiology 22: 323–326.
Wang, Z. Y., Nakano, T., Gendron, J., He, J. X., Chen, M., Vafeados, D., Yang, Y. L., Fujioka, S., Yoshida, S., Asami, T., Chory, J. (2002). Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis. Developmental Cell 2: 505–513
Wang, Z. Y., Seto, H., Fujioka, S., Yoshida, S., Chory, J. (2001). BRI1 is a critical component of a plasma-membrane receptor for plant steroids. Nature 410: 380–383.
Welch, J., Eswarakrishnan, S. (1991). Fluorine in Bioorganic Chemistry. John Wiley Sons, New York.
Yokota, T., Baba, J., Arima, M., Morita, M., Takahashi, N. (1983). Isolation and structures of new brassinolide-related compounds in higher plants. Tennen Yuki Kagob. Toronkai Koen Yoshishu 26: 70–77 [C. A. 100:48616].
Yokota, T., Nakayama, N., Wakisaka, T. (1994). 3-Dehydroteasterone, a 3,6 diketobrassinosteroid as a possible biosynthetic intermediate of brassinolide from wheat grain. Bioscience Biotechnology and Biochemistry 58: 1183–1185.
Xu, R., He, Y-J., Wang, Y-Q., Zhao, Y-J. (1994). Preliminary study of brassinosterone binding sites from mung bean epicotyls. Acta Phytophysiologica Sinica 20: 298–302.
Xu, W., Prugganan, M. M., Polisensky, D. H., Antosiewicz, D. M., Fry, S. C., Braam, J. (1995). Arabidopsis TCH4, regulated by hormones and the environment, encodes a xyloglucan endotransglycosylase. Plant Cell 7: 1555–1567.
Yin, Y., Wang, Z. Y., Mora-Garcia, S., Li, J., Yoshida, S., Asami, T., Chory, J. (2002). BES1 accumulates in the nucleus in response to brassinosteroids to regulate gene expression and promote stem elongation. Cell 109: 181–191.
Yokota, T. (1997). The structure, biosynthesis and function of brassinosteroids. Trends in Plant Science 2: 137–143.
Zanol, G. C., Fortes, G. R. L., Silva, J. B., Faria, J. T. C., Gottinari, R. A., Centellas, A. Q. (1998). Uso do ácido indolbutírico e do escuro no enraizamento in vitro do porta-enxerto de macieira Marubakaido. Ciência Rural, 28: 387–391.
Zullo, M. A. T., Adam, G. (2002). Brassinosteroid phytohormones–structure, bioactivity and applications. Brazilian Journal of Plant Physiology 14: 143–181.
Zullo, M. A. T., Kohout, L., De Azevedo, M. B. M. (2003). Some notes on the terminology of brassinosteroids. Plant Growth Regulation 39: 1–11.
Zurek, D. M., Clouse, S. D. (1994). Molecular cloning and characterization of a brassinosteroid-regulated gene from elongating soybean (Glycine max L.) epicotyls. Plant Physiology 104: 161–170.
Zurek, D. M., Rayle, D. L., McMorris, T. C., Clouse, S. D. (1994). Investigation of gene expression, growth kinetics, and wall extensibility during brassinosteroid-regulated stem elongation. Plant Physiology 104: 505–513.
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Pereira-Netto, A.B., Schaefer, S., Galagovsky, L.R., Ramirez, J.A. (2003). Brassinosteroid-Driven Modulation of Stem Elongation and Apical Dominance: Applications in Micropropagation. In: Hayat, S., Ahmad, A. (eds) Brassinosteroids. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0948-4_6
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