Abstract
Treatment of shoot apices of the marubakaido apple rootstock with a fluoro derivative of 28-homoethylcastasterone (5F-HCTS) in the range 100–10,000 ng per shoot increased the apple rootstock's multiplication rate (MR). A dose of 500 ng 5F-HCTS in 5 µl ethanol, per shoot, resulted in a significant (P=0.05) 112% increase in MR compared to shoots treated with 5 µl of 95% ethanol when the shoots were grown in culture medium enriched with 2.2 µM N6-benzyladenine. This increase in MR was due to: (1) a 15% increase in the number of main branches; (2) a 238% increase in the number of primary lateral branches; (3) a 250% increase in the number of secondary lateral branches. These results show that shoot proliferation induced by the fluoro derivative of 28-homocastasterone is an effective method to enhance the in vitro multiplication rate in Malus prunifolia.
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Schaefer, S., Medeiro, S., Ramírez, J. et al. Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]. Plant Cell Rep 20, 1093–1097 (2002). https://doi.org/10.1007/s00299-002-0442-3
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DOI: https://doi.org/10.1007/s00299-002-0442-3