Abstract
Conventional plant breeding is based on genetic variation and selection of the desired genotypes. The availability of genetic diversity and genetic variation is the starting point of any breeding programme. In most crops, sufficient genetic variation is present among land races, cultivars and their wild relatives. In conventional plant breeding programmes this is followed by several years of selection and field evaluation before a desired genotype is released as an improved cultivar. In many sexually propagated crops, e.g., rice, wheat, maize, barley, the genetic variability is usually recombined through hybridization. The desired recombinants are then selected from the segregating populations in the subsequent generations which are tested, multiplied and released as improved cultivars. However, a number of important crops such as banana, plantain, date palm, citrus, potato, sweet potato, pineapple, apple, and pear are propagated from vegetative parts, and are not amenable to improvement in the same manner as sexually propagated plants. Many of these plants are complex polyploids, take several years to flower and fruit, some are self-incompatible. In others, such as banana and plantain, there is little or no seed formation. Often the propagule size is too big and bulky to handle and to grow large populations. Hence, it is not possible to produce sufficiently large populations required to obtain the desired recombinant in a short duration. In these plants, mutation induction offers the possibility to alter a few characters without disrupting the genome while retaining all the other characters of clones.
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References
Ahloowalia, B.S. 1975. Regeneration of ryegrass plants in tissue culture. Crop Sci. 15: 449–452.
Ahloowalia, B.S. 1976. Chromosomal changes in parasexually produced ryegrass. In: Current Chromosome Research, pp. 115–122 (eds K. Jones and P. Brandham). Amsterdam: North-Holland
Ahloowalia, B.S. 1983. Spectrum of variation in somaclones of triploid ryegrass. Crop Sci. 23: 1141–1147.
Ahloowalia, B.S. 1986. Limitations to the use of somaclonal variation in crop improvement. In: Somaclonal Variation and Crop Improvement. pp. 14–27 (ed. J. Semal). Martinus Niihoff
Ahloowalia, B.S. 1990. In vitro radiation induced mutagenesis in potato. In: The Impact of Biotechnology in Agriculture, pp. 39–46 (eds R.S. Sangwan and B.S. Sangwan). Dordrecht: Kluwer.
Ahloowalia, B.S. 1992. In vitro mutation and multiplication of chrysanthemum cultivars. Farm Food. 2: 28–29.
Ahloowalia, B.S. 1994. Production and performance of potato mini-tubers. Euphytica. 75: 1163–172.
Ahloowalia, B.S. 1995. In vitro mutagenesis for the improvement of vegetatively propagated plants. In: Proceedings, ‘Induced Mutations and Molecular Techniques for Crop Improvement’, pp. 531–541. International Symposium, IAEA and Food Agriculture Organization of the United Nations, IAEA SM–340, Vienna.
Anonymous. 1977. Manual on Mutation Breeding, 2nd edn. Vienna: International Atomic Energy Agency, Tech. Rep. Ser. No. 119.
Behnke, M. 1979. Selection of potato callus for resistance to culture filtrates of Phytophthora infestans and regeneration of resistant plants. Theor Appl Genet. 55: 69–71.
Behnke, M. 1980. General resistance to blight of Solanum tuberosum plants regenerated from callus resistant to culture filtrates of Phytophthora infestans. Theor Appl Genet. 56: 151–152.
Bieniek, M.E., R.C. Harrell and D.J. Cantliffe. 1995. Enhancement of somatic embryogenesis of Ipomoea batatas in solid cultures and production of mature somatic embryos in liquid cultures for application to a bioreactor production system. Plant Cell Tissue Org. Cult. 41: 1–8.
Binding, H., K. Binding and J. Straub. 1970 Selektion in Gewebekulturen mit haploiden Zellen. Naturwissenschaften 3: 138–139.
Briggs, R.W. and M.J. Constantin. 1977. Radiation types and radiation sources. In: Manual on Mutation Breeding, 2nd edn, pp. 7–20. Vienna: International Atomic Energy Agency, Tech. Rep. Ser. No. 119.
Broertjes, C. 1977. Mutagen treatment and handling of treated material. In: Manual on Mutation Breeding, 2nd edn, pp. 160–168. Vienna: International Atomic Energy Agency, Tech. Rep. Ser. No. 119.
Brunner, H. and H. Keppl. 1991. Radiation induced apple mutants of improved commercial value. In: Proceedings, ‘Plant Mutation Breeding for Crop Improvement’ vol. 1, pp. 547–552. International Symposium, IAEA and Food Agriculture Organization of the United Nations, IAEA SM- 311, Vienna.
Carlson, P.S. 1973. Methionine sulfoxamine-resistant mutants of tobacco. Science. 180: 1366–1368.
Cassells, A.C., C. Walsh and C. Periappuram. 1993. Diplontic selection as positive factor in determining the fitness of mutants of Dianthus ‘Mystere’ derived from x-irradiation of nodes in in vitro cultures. Euphytica. 70: 167–174.
Chaleff, R.S. 1983. Isolation of agronomically useful mutants from plant cell cultures. Science. 219: 676–682.
Chaleff, R.S. and T.B. Ray. 1984. Herbicide-resistant mutants from tobacco cell cultures. Science. 223: 1148–1151.
Cheikh, R., A. Zaid, and M. Ait-Chitt. 1989. Travaux de récherches conduits sur l’embryogenese somatique chez le palmier dattier (Phoenix dactylifera L.). pp. 59–69. In Compte-Rendue Du Deuxieme Seminaire Maghrebin sur la Multiplication Rapide du Palmier Dattier par les Techniques de Culture In Vitro. FAO/UNDP/RAB/88/024. Marrakech: INRA.
Dale, P.J. 1995. R&D regulation and field trialling of transgenic crops. TIBTECH 13: 398–403.
Dix, P.J. and H.E. Street. 1975. Sodium chloride-resistant cultured cell lines from Nicotiana sylvestris and Capsicum annuum. Plant Sci Lett. 5: 231–237.
Dörffling, K., H. Dörffling and G. Lesselich. 1993. In vitro-selection and regeneration of hydroxyproline-resistant lines of winter wheat with increased proline content and increased frost tolerance. J Plant Physiol. 142: 222–225.
Drew, R.A. 1992. Improved techniques for in vitro propagation and germplasm storage of papaya. HortScience. 27: 1122–1124.
Duncan, R.R., R.M. Waskom and M.W. Nabors. 1995. In vitro screening and field evaluation of tissue culture regenerated sorghum (Sorghum bicolor (L.) Moench) for soil stress tolerance: recovery of improved genotypes. Euphytica. 85: 373–380.
Evans, D.A. and W.R. Sharp. 1983. Single gene mutations in tomato plants regenerated from tissue culture. Science. 221: 949–951.
Garcia de, E. and S. Martinez. 1995. Somatic embryogenesis in Solanum tuberosum L. cv. Desiree from stem nodal sections. J Plant Physiol. 145: 526–530.
Gegenbach, B.G., C.E. Green and C.M. Donovan. 1977. Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc Natl Acad Sci USA. 74: 5113–5117.
Grover, A., A. Pareek and S.C. Maheshwari. 1993. Molecular approaches for genetically engineering plants tolerant to salt stress. Proc Indian Natl Sci Acad. B59: 113–127.
Heinz, D.J., M. Krishnamurthi, L.G. Nickell and A.M. Maretzki. 1977. Cell tissue and organ culture in sugarcane improvement. Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture, pp. 3–17 (ed. Y.P.S. Bajaj). Berlin: Springer.
Hensz, R.A. 1991. Mutation breeding of grapefruit (Citrus paradisi Macf.). In: Plant Mutation Breeding for Crop Improvement, vol. 1. pp. 533–536. Vienna: IAEA.
Herbert-Soule, D., J.R. Rikkert and B.I. Reisch. 1995. Phosphinothricin stimulates somatic embryogenesis in grape (Vitis sp. L.). Plant Cell Rep. 14: 380–384.
Heslot, H. 1977. Chemical mutagens. Review of main mutagenic compounds. In: Manual on Mutation Breeding, 2nd edn, pp. 51–58. Vienna: International Atomic Energy Agency, Tech. Rep. Ser. No. 119.
Hsia, C.N. and S.S. Korban. 1996. Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis minima. Plant Cell Tissue Org Cult. 44: 1–6.
Jain, S., H.S. Nainawatee, R.K. Jain and J.B. Chowdhry. 1991. Proline status of genetically stable salttolerant Brassica juncea L. somaclones and their parent cv. Prakash. Plant Cell Rep. 9: 684–687.
Jehan, H., D. Courtois, C. Ehret, K. Lerch and V. Petiard. 1994. Plant regeneration of Iris pallida Lam. and Iris germanica L. via somatic embryogenesis from leaves, apices and young flowers. Plant Cell Rep. 13: 671–675.
Kim, J.W. and W.Y. Soh. 1996. Plant regeneration through somatic embryogenesis from suspension cultures of Allium fistulosum L. Plant Sci. 114: 215–220.
Kirti, P.B., S. Hadi, P.A. Kumar and V.L. Chopra. 1991. Production of sodium chloride-tolerant Brassica juncea plants by in vitro selection at the somatic embryo level. Theor. Appl. Genet. 83: 233–237.
Kobyashi, R.S., J.C. Bouwkamp and S.L. Sindan. 1994, Interspecific hybrids from cross incompatible relatives of sweet potato. Euphytica. 80: 159–164.
Kreuger, M., E. Postma, Y. Brouwer and G. van Holst. 1995. Somatic embryogenesis of Cyclamen persicum in liquid medium. Physiol Planta. 94: 605–612.
Krikorian, A.D. and S.S. Cronauer. 1984. Banana. Handbook of Plant Cell Culture, vol. 2: Crop Species, pp. 327–348 (eds W.R. Sharp, D.A. Evans, P. Ammirato and Y. Yamada). New York: Macmillan.
Lapade, A.G., A.M.S. Veluz and I.S. Santos. 1995. Genetic improvement of the Queen variety of pineapple through induced mutation and in vitro culture techniques. In: pp. 684–687. Proceedings, ‘Induced Mutations and Molecular Techniques for Crop Improvement’, International Symposium, IAEA and Food Agriculture Organization of the United Nations, IAEA, Vienna.
Lee, S.P. and H.H. Chen. 1993. Molecular cloning of abscisic acid-responsive mRNAs expressed during the induction of freezing tolerance in bromegrass (Bromus inermis Leyss) suspension cultures. Plant Physiol. 101: 1089–1096.
Love, S.L., T.P. Baker, A. Thompson-Johns and B.K. Werner. 1996. Induced mutations for reduced tuber glycoalkaloid content in potatoes. Plant Breed. 115: 119–122.
Mak, C., Y.W. Ho, Y.P. Tan and R. Ibrahim. 1996. Novaria – a new banana mutant induced by gamma irradiation. Infomusa. 5: 35–36.
Maliga, P. 1984. Isolation and characterization of mutants in plant cell cultures. Annu Rev Plant Physiol. 35: 519–552.
Maluszynski, M., B. Sigurbjornsson, E. Amano, L. Sitch and O. Kamra. 1992. Mutant varieties-data bank, FAO/IAEA database. Part II. Mutat Breed Newsl. 39: 14–17.
Maluszynski, M., B.S. Ahloowalia and B. Sigurbjornsson. 1995. Application of in vivo and in vitro mutation techniques for crop improvement. Euphytica. 85: 303–315.
Maribona, R.H. 1994. Department of BioPlantas, National Center for Scientific Research (CNIC), Havana, Cuba (personal communication).
Melchers, G. and L. Bergmann. 1959. Untersuchungen an Kulturen von haploiden Geweben von Antirrhinum majus. Ber Dtsch Bot Ges. 78: 21–29.
Miller, O.K. and K.W. Hughes. 1980. Selection of paraquat-resistant variants of tobacco from cell cultures. In Vitro 16: 1085–1091.
Mityko, J., A. Andrasfalvy and M. Fari. 1996. Hungarian pepper cultivar breaks through traditional barriers in breeding practices. Hung Agric Res. 1: 18–22.
Muller, A.J. 1983. Genetic analysis of nitrate reductase deficient tobacco plants regenerated from mutant cells. Evidence for duplicate structural genes. Mol Gen Genet. 192: 275–281.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Pl. 15: 473–497.
Nabors, M.W., S.E. Gibbs, C.S. Bernstein and M.E. Meis. 1980. NaCl-tolerant tobacco plants from cultured cells. Z Pflanzen Physiol. 97: 13–17.
Nabors, M.W. 1983. Increasing the salt and drought tolerance of crop plants. In: Current Topics in Plant Biochemistry and Physiology, vol. 2, pp. 165–184 (ed. D.D. Randall). Columbia: University of Missouri Press.
Sabapathy, S. and H. Nair. 1995. In vitro propagation of taro with spermine, arginine and ornithine. II. Plantlet regeneration via callus. Plant Cell Rep. 14: 520–524.
Sacristan, M.D. 1982. Resistance to Phoma lingam of plants regenerated from selected cell and embryogenic cultures of haploid Brassica napus. Theor Appl Genet. 61: 193–200.
Sanada, T., K. Kotobuki, T. Nishida, H. Fujita and F. Ikeda. 1993. A new Japanese cultivar ‘Golden Nijisseike’, resistant mutant to black spot disease of Japanese pear. Jpn J Breed. 43: 455–461.
Santos, I., I. Guimaraes and R. Salema. 1994. Somatic embryogenesis and plant regeneration of Nerium oleander. Plant Cell, Tissue Org Cult. 37: 83–86.
Schaik Van, C.E., A. Posthuma, M.J. De Jeu and E. Jacobsen. 1996. Plant regeneration through somatic embryogenesis from callus induced on immature embryos of Alstroemeria spp. L. Plant Cell Rep. 15: 377–380.
Sears, E.R. 1993. Use of radiation to transfer alien chromosome segments to wheat. Crop Sci. 33: 897–901.
Shah, D.M., R.B. Horsch, H.J. Klee, G.M. Kishore, J.A. Winater, N.E. Tumer, C.M. Hironaka, P.R. Sanders, C.S. Gasser, S. Aykent, N.R. Siegel, S.G. Rogers and R.T. Farley. 1986. Engineering herbicide tolerance in transgenic plants. Science. 233: 478–481.
Shanchun, C., G. Feng and Z. Jinren. 1991. Studies on the seedless character of citrus induced by irradiation. Mutat Breed Newsl. 37: 8–9.
Sharp, F.T. and G.W. Schaeffer. 1993. Distribution of amino acids in bran, embryo and milled endosperm and shifts in storage protein subunits of in vitro-selected and lysine-enhanced mutant and wild type rice. Plant Sci. 90: 145–154.
Sonnino, A. and P. Mini. 1993. Somatic embryogenesis in sweet potato. Acta Hort. 336: 239–243.
Stefaniak, B. 1994. Somatic embryogenesis and plant regeneration of Gladiolus (Gladiolus hort.). Plant Cell Rep. 13: 386–389.
Susheelamma, B.N., K. Raja Shear, A. Sarkar, M.R. Rao and R.K. Datta. 1996. Genotype and hormonal effects on callus formation and regeneration in mulberry. Euphytica. 90: 25–29.
Tisserat, B. 1984. Date Palm. In: Handbook of Plant Cell Culture, vol. 2: Crop Species, pp. 505–545 (eds W.R., Sharp, D.A. Evans, P. Ammirato and Y. Ynada). New York: Macmillan.
Tyagi, A.K., A. Rashid and S.C. Maheshwari. 1981. Sodium chloride resistant cell line from haploid Datura innoxia Mill. A resistance trait carried from cell to plantlet and vice versa in vitro. Protoplasma. 105: 327–332.
Vajrabhaya, M., T. Thanapaisal and T. Vajrabhaya. 1989. Development of salt tolerant lines of KDML and LPT rice cultivars through tissue culture. Plant Cell Rep. 8: 411–414.
Winicov, I. 1991. Characterization of salt tolerant alfalfa (Medicago sativa L.) plants regenerated from salt tolerant cell lines. Plant Cell Rep. 10: 561–564.
Yamaguchi-Shinozaki, K. and K. Shinozaki. 1993. The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana. Mol Gen Genet. 238: 17–25.
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Ahloowalia, B.S. (1998). In-vitro Techniques and Mutagenesis for the Improvement of Vegetatively Propagated Plants. In: Jain, S.M., Brar, D.S., Ahloowalia, B.S. (eds) Somaclonal Variation and Induced Mutations in Crop Improvement. Current Plant Science and Biotechnology in Agriculture, vol 32. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9125-6_15
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DOI: https://doi.org/10.1007/978-94-015-9125-6_15
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