Abstract
Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cellular salt tolerance characteristic can be passaged through the regenerated plants, since callus cultures initiated from immature ovaries of the salt tolerant regenerated plants are salt tolerant without additional selection on 1% NaCl. Several of these “second generation” callus cultures have been regenerated to produce vigorous plants which maintain the salt tolerance characteristic. The tolerance phenotype appears dominant in seeds obtained from self fertilization of the tolerant plants. The regenerated salt tolerant plants are therefore a valuable source as genotypes in plant breeding for salt tolerance and isolation, identification and manipulation of genes which confer salt tolerance in alfalfa.
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Abbreviations
- SH:
-
Schenk and Hildebrandt medium
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
References
Bingham ET, McCoy TJ (1986) Plant Breeding Rev. 4:123–152
Chandler SF, Vasil IK (1984) Plant Science Lett. 37:157–164
Croughan TP, Stavarek SJ, Rains DW (1978) Crop Science 18:959–963
Cushman JC, Meyer G, Michalowski CB, Schmitt JM, Bohnert HJ (1989) Plant Cell 1:715–725
Dobrenz AK, Schonhorst MH, Stone JE, Thompson RK, Allen S, Smith D (1983) Crop Science 23:807
Groose RW, Bingham ET (1986) Plant Cell Reports 5:104–107
Handa AK, Bressan RA, Handa S, Hasegawa PM (1983a) Plant Physiol. 72:645–653
Handa S, Bressan RA, Handa AK, Carpita NC, Hasegawa PM (1983b) Plant Physiol. 73:834–843
Hoagland DR, Arnon DI (1938) Univ. Calif. Berkeley Agric. Exp. Sta. Circ. 347:39
Larkin PJ, Ryan SA, Brettell RIS, Scowcroft WR (1984) Theor. Appl. Genet. 67:443–455
McCoy TJ (1987a) Plant Cell Reports 6:31–34
McCoy TJ (1987b) Plant Cell Reports 6:417–422
McCoy TJ, Phillips RL, Rines HW (1982) Can. J.Gen.Cytol. 24:37–50
McCoy T, Walker K (1984) in Handbook of Plant Cell Culture, v.3., eds. Ammirato PV, Evans DA, Sharp WR, Yamada Y. McMillan, New York, NY pp 171–192
Meredith CP (1984) in Gene Manipulation in Plant Improvement. 16th Stadler Genetics Symposium ed. Gustafson JP. Plenum Press, New York, N.Y. pp 503–528
Nabors MW, Dykes TA (1985) in Biotechnology in International Agricultural Research. International Rice Research Institute, Manila, Philippines pp 121–138
Nabors MW, Gibbs SE, Bernstein CS, Meis ME (1980) Z. Pflanzenphysiol. 97:13–17
Nabors MW, Kroskey CS, McHugh DW (1982) Z. Pflanzenphysiol. 105:341–349
Schenk RU, Hildebrandt AC (1972) Can. J. Bot. 50:199–204
Smith MK, McComb JA (1983) Plant Cell Rep. 2:126–128
Stavarek SJ, Rains DW (1984a) Hort. Science 19(3):377–382
Stavarek SJ, Rains DW (1984b) in Salinity Tolerance in Plants: Strategies for Crop Improvement, eds. Staples RC, Toenniessen GH. Wiley-Interscience. pp 321–334
Tal M (1983) in Handbook for Plant Cell Culture v.1. eds. Evans DA, Sharp WR, Ammirato PV, Yamada Y. McMillan N. Y., N.Y. pp 461–488
Waterborg JH, Harrington RE, Winicov I (1989) Plant Physiol. 90:237–245
Winicov I, Waterborg JH, Harrington RE, McCoy TJ (1989) Plant Cell Reports 8:6–11
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Communicated by I. K. Vasil
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Winicov, I. Characterization of salt tolerant alfalfa (Medicago sativa L.) plants regenerated from salt tolerant cell lines. Plant Cell Reports 10, 561–564 (1991). https://doi.org/10.1007/BF00232511
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DOI: https://doi.org/10.1007/BF00232511