Abstract
The Agrobacterium germ-line transformation method is a relatively new and immensely successful procedure for plant transformation. The method was originally developed by Nicole Bechtold, Jeff Ellis and Georges Pelletier [2]. Previous versions of this method have been known as ‘Agrobacterium vacuum infiltration’ or ‘In planta transformation,’ but these names are inaccurate or redundant with the names for other transformation methods. The primary reasons for the popularity of the method are its simplicity and high reliability: Minimal hands-on time is required and success can be achieved by persons who have no training in plant tissue culture techniques. Transformed plants can be obtained at such high rates that the procedure can be used not only to introduce specific gene constructs into plants, but also as a random mutagenesis method for genetagging [1]. The primary drawback of the method is that successful application has only been reported for one species: Arabidopsis thaliana.
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© 1998 Springer Science+Business Media Dordrecht
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Bent, A.F., Clough, S.J. (1998). Agrobacterium Germ-Line Transformation: Transformation of Arabidopsis without Tissue Culture. In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5242-6_2
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DOI: https://doi.org/10.1007/978-94-011-5242-6_2
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