Abstract
Modification of the rice genome by Agrobacterium-mediated transformation is a general technique that can be easily performed today. Successful methods were established by vigorous studies on the culture system and the elucidation of Agrobacterium transformation mechanisms. This section provides a detailed description of routine and efficient rice transformation protocols by Agrobacterium. This method uses mature seeds as a material and can be applied to many japonica and some other varieties of rice. According to this method, it is even possible for beginners to obtain rice transformants.
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Acknowledgments
The author would like to thank Dr. Makoto Matsuoka, Dr. Nobuhiro Tsutsumi, and the Honda Research Institute and Japan Society for Promotion of Science (JSPS) Fellowship for supporting research on this protocol.
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Nishimura, A. (2020). Agrobacterium Transformation in the Rice Genome. In: Vaschetto, L. (eds) Cereal Genomics. Methods in Molecular Biology, vol 2072. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9865-4_17
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DOI: https://doi.org/10.1007/978-1-4939-9865-4_17
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