Skip to main content

Isolation, characterization and associative N-fixation of acid-tolerant Azospirillum brasilense strains associated with Eleusine coracana in low pH-Al-rich acid soil

  • Chapter
Plant-Soil Interactions at Low pH

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 45))

Abstract

Five Azospirillum brasilense strains were isolated from the roots of finger millet (Eleusine coracana L.) plants grown in acid soils of Chhota Nagpur region of Bihar. These strains were characterized for their shape, size, motality, strain specific chemotaxis and nitrogen fixation ability in liquid medium containing different concentrations of AlCl3 and MnCl3 at different pH levels. These strains also showed cross-resistance to neomycin and penicillin but not to chloramphenicol and tetracyclin.

The use of various carbon substrate for N2-dependent growth and nitrogenase activity were also determined. Response of inoculation with these acid-tolerant strains was variable with different genotypes of finger millet in sterile acid soil. These results demonstrate that acid-tolerant strains may be most suitable for nitrogen economy, mineral uptake and plant growth in acid soils having various pH and associated factors of acidity.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 429.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 549.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 949.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Alder J 1966 Chemotaxis in bacteria. Science. 153, 709–716.

    Google Scholar 

  • Alvarez-Morales R A and Lemos-Postrana P 1980 Chemotaxis of Azospirillum lipoferum and Azospirillum brasilense towards root exudates of Gramineae. Rev. Latinoam. Microbial. 22, 131–136.

    CAS  Google Scholar 

  • Barak R, Nur I and Okon Y 1983 Detection of chemotaxis in Azospirillum brasilense. J. Appl. Bacteriol. 152, 399–403.

    Google Scholar 

  • Boddey R M and Döbereiner J 1982 Association of Azospirillum and other diazotrophs with tropical Graminae. In Non-Symbiotic Nitrogen Fixation and Organic Matter in the Tropics. 12th International Congress of Soil Science, New Delhi, India. Papers Vol. 1, pp 28–47.

    Google Scholar 

  • Caceres E A R 1982 Improved medium for isolation of Azospirillum spp. Appl. Environ. Microbiol. 44, 990–991.

    PubMed  CAS  Google Scholar 

  • Dart P J, Day J, Islam R and Döbereiner J 1976 Symbiosis in tropical grain legumes: Some aspects of temperature and composition of the rooting medium. In Symbiotic Nitrogen Fixation in Plants. Ed. P. S. Nutman, pp 361–383. Cambridge University Press, Cambridge.

    Google Scholar 

  • Döbereiner J, Marriel I E and Nery M 1976 Ecological distribution of Spirillum lipoferum Beijerinck. Can. J. Microbiol. 22, 1464–1473.

    Article  PubMed  Google Scholar 

  • Jackson M L 1978 Soil Chemical Analysis. Indian Edition. Prentice Hall of India Pvt. Ltd., New Delhi.

    Google Scholar 

  • Kapulnik Y, Feldman M, Okon Y and Henis Y 1985a Contribution of nitrogen fixed by Azospirillum to the N nutrition of spring wheat in Isreal. Soil Biol. Biochem. 17, 509–516.

    Article  Google Scholar 

  • Kapulnik Y, Okon Y and Henis Y 1985 Changes in root morphology of wheat caused by Azospirillum inoculation. Can. J. Microbiol. 31, 881–887.

    Article  Google Scholar 

  • Ladha J K, So R B and Watanabe I 1988 Composition of Azospirillum species associated with wetland rice plant grown in different soil. Plant and Soil 12, 127–129.

    Google Scholar 

  • Lakshmi-Kumari M, Kavimandan S K and Subba Rao N S 1976 Occurrence of nitrogen fixing Spirillum in roots of rice, sorghum, maize and other plants. Ind. J. Exp. Biol. 14, 638–639.

    Google Scholar 

  • Lin W, Okon Y and Hardy R W F 1983 Enhanced mineral uptake by Zea mays and Sorghum bicolor roots inoculated with Azospirillum brasilense. Appl. Environ. Microbiol. 45, 1775–1779.

    PubMed  CAS  Google Scholar 

  • Okon Y, Albrecht S L and Burris R H 1977 Methods for growing Spirillum lipoferum and for counting it in pure culture and in association with plants. Appl. Environ. Microbiol. 33, 85–88.

    PubMed  CAS  Google Scholar 

  • Okon Y and Kapulnik Y 1986 Field inoculation studies with Azospirillum in Israel. Plant and Soil 90, 3–16.

    Article  CAS  Google Scholar 

  • Patriquin D C, Döbereiner J and Jain D K 1983 Sites and processes of association between diazotrophs and grasses. Can. J. Microbiol. 29, 900–915.

    Article  Google Scholar 

  • Pedroantonio A P, Döbereiner J and Neyara C A 1981 Nitrogen assimilation and dissimilation in five genotypes of Brachiaria spp. Can. J. Microbiol 59, 1475–1479.

    Google Scholar 

  • Rai R 1983 Efficency of associative N2-fixation by streptomycin-resistant mutants of Azospirillum brasilense with genotypes of chickpea Rhizobium strains. J. Agric. Sci. Camb. 100, 75–80.

    Article  CAS  Google Scholar 

  • Rai R 1984 Interaction between finger millet (Eleusine coracana) genotypes and drug-resistant mutants of Azospirillum brasilense in calcareous soil. J. Agric. Sci. Camb. 102, 521–527.

    Article  CAS  Google Scholar 

  • Rai R 1985 Studies on nitrogen fixation by antibiotic-resistant mutants of Azospirillum brasilense and their interaction with Cheena (Panicum miliaceum L.) genotypes in calcareous soil. J. Agric. Sci. Camb. 105, 261–270.

    Article  CAS  Google Scholar 

  • Rai R 1986 Manganese-mediated resistant to aluminium and antibiotics in strains of Azospirillum brasilense and their interaction with rice genotypes in acid soil. J. Agric. Sci. Camb. 106, 279–285.

    Article  CAS  Google Scholar 

  • Rai R 1985 Manganese-resistant mutants of Azospirillum brasilense: Their growth and relative efficiency in associative nitrogen fixation with cheena (Panicum miliaceum L.) in acid soil. J. Gen. Appl. Microbiol. 31, 211–219.

    Article  CAS  Google Scholar 

  • Rai R 1985 Studies on nitrogen fixation by machete-resistant mutants strains of A. brasilense: Their associative N2-fixation and yield response of rice to root inoculation in calcareous soil. J. Gen. Appl. Microbiol. 31, 1–16.

    Article  CAS  Google Scholar 

  • Rai R 1986 Chemotaxis of Cicer Rhizobium strains to root exudates of chickpea (Cicer arietinum L.) genotypes and their interaction response on nodulation, noduline leghaemoglobion and grain yield in calcareous soil. J. Agric. Sci. Camb. 107, 437–444.

    Google Scholar 

  • Rai R 1987 Chemotaxis of salt-tolerant and sensitive Rhizobium strains to root exudates of lentil (Lens culinaris L.) genotypes and symbiotic N-fixation, proline content and grain yield in saline calcareous soil. J. Agric. Sci. Camb. 108, 25–37.

    Article  CAS  Google Scholar 

  • Rai R 1988 High-temperature-adapted Azospirillum brasilense strains: growth and interaction response on associative nitrogen fixation, mineral uptake and yield of cheena (Panicum miliaceum L.) genotypes in calcareous soil. J. Agric. Sci. Camb. 110, 321–329.

    Article  Google Scholar 

  • Rai R 1988 Manganese-resistant mutants of Azospirillum brasilense: their response of associative nitrogen fixation, dry matter production and nitrogen content of cheena (Panicum miliaceum L.) to different amounts of applied nitrogen in acid soil. J. Agric. Sci. Camb. 110, 81–92.

    Article  CAS  Google Scholar 

  • Rai R 1988 Effect of Azospirillum brasilense strains on the iron uptake and N2-fixation by roots of cheena (Panicum miliaceum L.) genotypes. J. Plant Nutr. 11, 871–879.

    Article  CAS  Google Scholar 

  • Smith R L, Schank S C, Milam J R and Boltensperger A A 1984 Response of Sorghum and Pannisetum species to the N2 fixing bacterium Azospirillum brasilense, Appl. Environ Microbiol. 47, 1331–1336.

    CAS  Google Scholar 

  • Tien T M, Gaskins M H and Hubbel D M 1979 Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet. Appl. Environ. Microbiol. 37. 1016–1024.

    PubMed  CAS  Google Scholar 

  • Tien T M, Tien H G, Gaskins M H and Hubbel D H 1982 Polygalacturonic acid transeliminase production by Azospirillum spp. Can. J. Microbiol. 27, 426–431.

    Article  Google Scholar 

  • Wani S P 1985 Nitrogen fixation potentials of sorghum and millet. In Biological Nitrogen Fixation Recent Development. Ed. N S Subba Rao, pp 125–175. Oxford & IBH Publications, India.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Rai, R. (1991). Isolation, characterization and associative N-fixation of acid-tolerant Azospirillum brasilense strains associated with Eleusine coracana in low pH-Al-rich acid soil. In: Wright, R.J., Baligar, V.C., Murrmann, R.P. (eds) Plant-Soil Interactions at Low pH. Developments in Plant and Soil Sciences, vol 45. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3438-5_75

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-3438-5_75

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5520-8

  • Online ISBN: 978-94-011-3438-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics