Abstract
Xanthan is typically produced copiously as extracellular slime polysaccharide by the various pathovars of X. campestris and by some other Xanthomonas species. The production of this exopolysaccharide is manifested in the large mucoid colonies of Xanthomonas species on solid media and in the viscosity of liquid cultures. In the laboratory, the polysaccharide can be readily prepared in high yield then separated from the bacterial cells by high speed centrifugation. It is also an industrial product manufactured on a large scale by batch fermentation, the current estimated world production being at least 20 000 tonnes per annum.
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References
Betlach, M.R., Capage, M.A., Doherty, D.H., et al. (1987) Genetically engineered polymers: manipulation of xanthan biosynthesis, in Industrial Polysaccharides (ed. M. Yalpani), Elsevier, Amsterdam, pp. 145–56.
Boulnois, G.J. (1989) Genetics of capsular production in bacteria. Society for Experimental Biology Symposium, 63, 417–22.
Boulnois, G.J. and Jann, K. (1989) Bacterial polysaccharide capsule synthesis, export and evolution of structural diversity. Molecular Microbiology, 3,1819–23.
Cadmus, M.C., Jackson, L.K., Burton, K.A., Plattner, R.D. and Slodki, M.E. (1982) Biodegradation of xanthan gum by Bacillus sp. Applied and Environmental Microbiology, 44, 5–11.
Davidson, I.W. (1978) Production of polysaccharide by Xanthomonas campestris in continuous culture. FEMS Microbiology Letters, 3, 347–9.
Haaheim, L. and Sutherland, I.W. (1989) Monoclonal antibodies reacting with the exopolysaccharide from Xanthomonas campestris. Journal of General Microbiology, 135, 605–12.
Ho, C.S., Ju, L.-K. and Baddour, R.F. (1988) The anomaly of oxygen diffusion in aqueous xanthan solutions. Biotechnology and Bioengineering, 32, 8–17.
Ielpi, L., Couso, R. and Dankert, M. (1981) Lipid-linked intermediates in the biosynthesis of xanthan gum. FEBS Letters, 130, 253–6.
Ielpi, L., Couso, R.O. and Dankert, M. (1983) Xanthan gum biosynthesis acetylation occurs at the prenyl-phospho-sugar stage. Biochemical International, 6, 323–33.
Jansson, P.-E., Kenne, L. and Lindberg, B. (1975) Structure of the extracellular polysaccharide from Xanthomonas campestris. Carbohydrate Research, 45, 275–82.
Jarman, T.R. and Pace, G.W. (1984) Energy requirements for microbial exopolysaccharide synthesis. Archives of Microbiology, 137, 231–5.
Kang, K.S. and Cottrell, I.W. (1979) Polysaccharides, in Microbial Technology, Vol. I (eds H.J. Peppler and D. Perlman), Academic Press, New York, pp. 417–81.
Kierulf, C. and Sutherland, I. W. (1988) Thermal stability of xanthan preparations. Carbohydrate Polymers, 9, 185–94.
Lawson, C.J. and Symes, K.C. (1977) Oligosaccharides produced by partial acetolysis of xanthan gum. Carbohydrate Research, 58, 433–8.
Linton, J.D. (1990) The relationship between metabolite production and the growth efficiency of the producing organism. FEMS Microbiological Review, 75, 1–18.
Melton, L.D., Mindt, L., Rees, D.A. and Sanderson, G.R. (1976) Covalent structure of the extracellular polysaccharide from Xanthomonas campestris. Carbohydrate Research, 46, 245–57.
Moorhouse, R., Walkinshaw, M.D. and Arnott, S. (1977) Xanthan gum — molecular conformation and interactions. ACS Symposium, 45, 90–102.
Morris, V.J. and Miles, M. (1986) Effect of natural modifications on the functional properties of extracellular bacterial polysaccharides. International Journal of Biol. Macromol., 8, 342–8.
Nakajima, S., Funahashi, H. and Yoshida, T. (1990) Xanthan gum production in a fermentor with twin impellors. Journal of Fermentation and Bioengineering, 70, 392–7.
Norisuye, T., Yanaki, T. and Fujita, H. (1980) Triple helix of a Schizophyllum commune polysaccharide in aqueous solution. Journal of Polymer Science, 18, 547–8.
Rye, A.J., Drozd, J.W., Jones, C.W. and Linton, J.D. (1988) Growth efficiency of Xanthomonas campestris in continuous culture. Journal of General Microbiology, 134, 1055–61.
Shatwell, K.P. (1989) The influence of acetyl and pyruvic acid substituents on the solution and interaction properties of xanthan. PhD thesis, Edinburgh University.
Shatwell, K.P., Sutherland, I.W., Dea, I.C.M. and Ross-Murphy, S.B. (1990) The influence of acetyl and pyruvate substituents on the solution properties of xanthan polysaccharide. Carbohydrate Research, 206, 87–103.
Shatwell, K.P., Sutherland, I.W., Ross-Murphy, S.B. and Dea, I.C.M. (1991) Influence of the acetyl substituent on the interaction of xanthan with plant polysaccharides. I. Xanthan-locust bean gum systems. Carbohydrate Polymers, 14, 29–51.
Silman, R.W. and Rogovin, P. (1972) Continuous fermentation to produce xanthan biopolymer: effect of dilution rate. Biotechnol. Bioengin., 14, 23–31.
Stokke, B.T., Foss, P., Christensen, B.E., Kierulf, C. and Sutherland, I.W. (1989) Long-term storage of xanthan in seawater at elevated temperature: physical dimensions and chemical composition of degradation products. International Journal of Biol. Macromol., 11, 137–44.
Suh, I.-S., Herbst, H., Schumpe, A. and Deckwer, W.-D. (1990) The molecular weight of xanthan polysaccharide produced under oxygen limitation. Biotechnology Letters, 12, 201–6.
Sutherland, I.W. (1987) Xanthan lyases — novel enzymes found in various bacterial species. Journal of General Microbiology, 133, 3129–34.
Tait, M.I. and Sutherland, I.W. (1989) Synthesis and properties of a mutant type of xanthan. Journal of Applied Bacteriology, 66, 457–60.
Tait, M.I., Sutherland, I.W. and Clarke-Sturman, A.J. (1986) Effect of growth conditions on the production, composition and viscosity of Xanthomonas campestris exopolysaccharide. Journal of General Microbiology, 132, 1483–92.
Tang, J.-L., Gough, C.L. and Daniels, M.J. (1990) Cloning of genes involved in negative regulation of production of extracellular enzymes and polysaccharide of Xanthomonas campestris pathovar campestris. Molecular and General Genetics, 222, 157–60.
Tang, J.-L., Liu, Y.-N., Barber, C.E., Dow, J.M., Wootton, J.C. and Daniels, M.J. (1991) Genetic and molecular analysis of a cluster of rpf genes involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pathovar campestris. Molecular and General Genetics, 226, 409–17.
Vanderslice, R.W., Doherty, D.H., Capage, M., et al. (1989) Genetic engineering of polysaccharide structure in Xanthomonas campestris, in Biomedical and Biotechnological Advances in Industrial Polysaccharides (eds V. Crescenzi, I.C.M. Dea, S. Paoletti, S.S. Stivala and I.W. Sutherland), Gordon and Breach, New York, pp. 145–56.
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© 1993 Springer Science+Business Media Dordrecht
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Sutherland, I.W. (1993). Xanthan. In: Swings, J.G., Civerolo, E.L. (eds) Xanthomonas. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1526-1_8
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DOI: https://doi.org/10.1007/978-94-011-1526-1_8
Publisher Name: Springer, Dordrecht
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