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Genomic DNA Isolation, Southern Blotting and Hybridization

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Plant Molecular Biology — A Laboratory Manual

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Abstract

The isolation of plant nucleic acids is a fundamental requirement for most genome characterization and mapping procedures involving the use of genetic markers, and for the identification and isolation of plant genes for genetic engineering. The degree of purity and quality required of the DNA isolated varies from application to application. On the one hand, high molecular weight, high purity DNA is required for the production of genomic DNA libraries, which are screened for plant gene sequences and for other genetic markers such as RFLPs. For genetic analysis, on the other hand, the degree of purity required may be lower, but other factors, such as the yield of DNA, may be more important.

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Authors
  1. S. Wilkie
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  2. M. S. Clark
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  3. P. Leroy
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  4. M. Merlino
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  5. S. Nègre
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  6. J. C. Caissard
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  7. P. Sourdille
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  8. Y. H. Lu
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  9. M. Bernard
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Editor information

Editors and Affiliations

  1. Molecular Genetics Unit, School of Clinical Medicine, Department of Medicine, Level 5, Addenbrooke’s Hospital, University of Cambridge, Hills Road, Cambridge, CB2 2QQ, UK

    Melody S. Clark

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© 1997 Springer-Verlag Berlin Heidelberg

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Wilkie, S. et al. (1997). Genomic DNA Isolation, Southern Blotting and Hybridization. In: Clark, M.S. (eds) Plant Molecular Biology — A Laboratory Manual. Springer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87873-2_1

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  • DOI: https://doi.org/10.1007/978-3-642-87873-2_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-49144-3

  • Online ISBN: 978-3-642-87873-2

  • eBook Packages: Springer Book Archive

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