Abstract
Genetic maps of the homoeologous group-6 chromosomes of bread wheat, Triticum aestivum, have been constructed spanning 103 cM on 6A, 90 cM on 6B and 124 cM on 6D. These maps were transferred to a Chinese Spring (CS) x line #31 cross to locate a dominant powdery mildew resistance gene, Pm12, introgressed into line #31 from Aegilops speltoides. Pm12 was shown to lie on the short arm of translocation chromosome 6BS-6SS.6SL in line #31, but could not be mapped more precisely due to the lack of recombination between the 6S Ae. speltoides segment and chromosome 6B. Possible strategies to reduce the size of the alien segment, which probably encompasses the complete long arm and more than 82% of the short arm of chromosome 6B, are discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bartels D, Thompson RD (1983) The characterisation of cDNA clones coding for wheat storage proteins. Nucleic Acids Res 11:2961–2977
Baulcombe DC, Barker RF, Jarvis MG (1987) A gibberellin-responsive wheat gene has homology to yeast carboxypeptidase Y. J Biol Chem 262:13726–13735
Bennett FGA (1984) Resistance to powdery mildew in wheat: a review of its use in agriculture and breeding programmes. Plant Pathology 33:279–300
Bethards LA, Skadsen RW, Scandalios JG (1987) Isolation and characterization of a cDNA clone for the Cat2 gene in maize and its homology with other catalases. Proc Natl Acad Sci 84:6830–6834
Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD (1989) RFLP-based genetic maps of wheat homoeologous group-7 chromosomes. Theor Appl Genet 78:495–504
Cheng CL, Dewdney J, Kleinhofs A, Goodman HM (1986) Cloning and nitrate induction of nitrate reductase mRNA. Proc Natl Acad Sci 83:6825–6828
Devos KM, Atkinson MD, Chinoy CN, Liu C, Gale MD (1992) RFLP-based genetic map of the homoeologous group-3 chromosomes of wheat and rye. Theor Appl Genet 83:931–939
Devos KM, Millan T, Gale MD (1993) Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley. Theor Appl Genet 85:784–792
Devos KM, Bryan GJ, Collins AJ, Stephenson P, Gale MD (1995) Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor Appl Genet 90:247–252
Dvořák J, Chen K-C (1984) Distribution of non-structural variation between wheat cultivars along chromosome arm 6Bp: evidence from the linkage and physical map of the arm. Genetics 106: 325–333
Gerlach WL, Bedbrook JR (1979) Cloning and characterisation of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885
Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374
Guiltinan MJ, Marcotte WR Jr, Quatrano RS (1990) A plant leucine zipper protein that recognizes an abscisic acid response element. Science 250:267–271
Gulli M, Maestri E, Hartings H, Raho G, Perrotta C, Devos KM, Marmiroli N (1995) Isolation and characterization of abscisic acid-inducible genes in barley seedlings and their responsiveness to environmental stress. Life Sci Advances — Plant Physiol 14:89–96
Harcourt RL (1992) DNA sequence polymorphisms in Triticeae species. PhD thesis, Cambridge University, UK
Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio BA, Shomura A, Shimizu T, Lin S-Y, Inoue T, Fukuda A, Shimano T, Kuboki Y, Toyama T, Miyamoto Y, Kirihara T, Hayasaka K, Miyao A, Monna L, Zhong HS, Tamura Y, Wang Z-X, Momma T, Umehara Y, Yano M, Sasaki T, Minobe Y (1994) A 300-kilobase-interval genetic map of rice including 883 expressed sequences. Nature Genetics 8:365–372
Lazarus CM, Baulcombe DC, Martienssen RA (1985) α-Amylase genes of wheat are two multigene families which are differentially expressed. Plant Mol Biol 5:13–24
McFadden ES, Sears ER (1946) The origin of Triticum spelta and its free-threshing hexaploid relatives. J Hered 37:81–89
Miller TE, Reader SM, Ainsworth CC, Summers RW (1988) The introduction of a major gene for resistance to powdery mildew of wheat, Erysphe graminis f. sp. tritici, from Aegilops speltoides into wheat, Triticum aestivum. In: Jorna ML, Slootmaker LAJ (eds) Cereal breeding related to integrated cereal production. Pudoc, Netherlands, pp 179–183
Priestley RH, Bayles RA (1988) The contribution and value of resistant cultivars to disease control in cereals. In: Clifford BC, Lester E (eds) Control of plant diseases: costs and benefits. Blackwell Scientific Publications, Oxford, pp 53–65
Riley R, Unrau J, Chapman V (1958) Evidence on the origin of the B genome of wheat. J Hered 49:91–98
Sears ER (1954) The aneuploids of common wheat. Mo Agric Exp Stn Res Bull 572:1–59
Sears ER (1976) A synthetic hexaploid wheat with fragile rachis. Wheat Inf Serv 41–42:31–32
Sears ER, Sears LMS (1979) The telocentric chromosomes of common wheat. In: Ramanujam S (ed) Proc 5th Int Wheat Genet Symp. The Indian Society of Genetics and Plant Breeding, Indian Agricultural Research Institute, New Delhi, pp 389–407
Author information
Authors and Affiliations
Additional information
Communicated by G. E. Hart
Rights and permissions
About this article
Cite this article
Jia, J., Devos, K.M., Chao, S. et al. RFLP-based maps of the homoeologous group-6 chromosomes of wheat and their application in the tagging of Pm12, a powdery mildew resistance gene transferred from Aegilops speltoides to wheat. Theoret. Appl. Genetics 92, 559–565 (1996). https://doi.org/10.1007/BF00224558
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224558