Skip to main content

Enzymes Involved in Intracellular Metabolism

  • Chapter
Methods in Soil Biology
  • 1208 Accesses

Abstract

The catalase of aerobic organisms splits the toxic H2O2 produced from the mitochondrial electron transport and from various hydroxylation and oxygenation reactions into water and oxygen. Since aerobic organisms predominate in non-compacted and non-waterlogged soils, catalase activity was used to characterize soil microbial activities (Beck 1971). Catalase was also the first enzyme that was investigated in soils (Woods 1899, cited in Skujins 1978). While Kuprevich and Shcherbakova (1956, 1971) and Beck (1971) determined the activity via the produced oxygen, Baroccio (1958) and Johnson and Temple (1964) measured the unused H2O2. Soil catalase is characterized by its high persistence. Fresh root material distorts microbial catalase activity measurements since plants also contain catalase.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

References

  • Baroccio A (1958) Catalase activity of soil as a biopedological index of fertility. Agrochimica 2: 243 – 25.

    Google Scholar 

  • Beck T (1971) Die Messung der Katalaseaktivität von Böden. Z Pflanzenernaehr Bodenkd 130: 68 – 81

    Article  CAS  Google Scholar 

  • Benefield CB, Howard PJA, Howard DM (1977) The estimation of dehydrogenase activity in soil. Soil Biol Biochem 9:67–70

    Article  CAS  Google Scholar 

  • Casida LE Jr, Klein DA, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371– 376

    Article  CAS  Google Scholar 

  • Curl H, Sandberg J (1961) The measurement of dehydrogenase activity in marine organisms. J Mar Res 19:123–138

    CAS  Google Scholar 

  • Griffiths BS (1989) Improved extraction of iodonitrotetrazolium-formazan from soil with dimethylformamide. Soil Biol Biochem 21:179–180

    Article  CAS  Google Scholar 

  • Johnson JL, Temple KL (1964) Some variables affecting the measurement of “catalase Activity” in soil. Soil Sci Soc Am Proc 28:207–209

    Article  CAS  Google Scholar 

  • Kuprevich VF, Shcherbakova TA (1956) Determination of invertase and catalase activity of soils. Vestsi Akad Navuk Belarusk SSR, Ser Biyal 2: 115 – 116

    Google Scholar 

  • Kuprevich VF, Shcherbakova TA (1971) Soil Enzymes. US Department of Commerce. National Technical Information Service, Springfield, Virginia

    Google Scholar 

  • Lenhard G (1956) Die Dehydrogenaseaktivitat des Bodens als Maß für die Mikroorganismentätigkeit im Boden. Z Pflanzenernaehr Bodenkd 73: 1 – 11

    Article  CAS  Google Scholar 

  • Skujins J (1978) History of abiontic soil enzyme research. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 1 – 49

    Google Scholar 

  • Stevenson IL (1959) Dehydrogenase activity in soils. Can J Microbiol 5: 229 – 235

    Article  CAS  Google Scholar 

  • Tabatabai MA (1982) Soil enzymes. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, part 2. Am Soc Agron, Soil Sci Soc Am, Madison, Wiscinson, pp 903 – 947

    Google Scholar 

  • Thalmann A (1968) Zur Methodik der Bestimmung der Dehydrogenaseaktivität im Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtsch Forsch 21:249– 258

    Google Scholar 

  • Trevors JT (1984) Dehydrogenase activity in soil: a comparison between the INT and TTC assay. Soil Biol Biochem 16: 673 – 674

    Article  CAS  Google Scholar 

  • von Mersi W, Schinner F (1991) An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol Fertil Soils 11: 216 – 220

    Article  Google Scholar 

  • Woods AF (1899) The destruction of chlorophyll by oxidizing enzymes. Zentralbl Bakteriol Parasitenkd Abt 2 5: 745 – 754

    Google Scholar 

References

  • Beck T (1971) Die Messung der Katalaseaktivität von Böden. Z Pflanzenernaehr Bondenkd 130; 38 – 81

    Google Scholar 

  • Stefanic G, Beck T, Schwemmer J, Hartmann F, Varbancin A (1984) Apparatus for measuring soil catalase activity. 5th Symp Soil Biol, JASJ Buckarest: 47 – 50

    Google Scholar 

  • Trevors JT (1984) Rapid gaschromatographic method to measure H2O2 Oxidoreductase activity in soil. Soil Biol Biochem 16: 525 – 526

    Article  CAS  Google Scholar 

References

  • Glathe H, Thalmann H (1970) Über die mikrobielle Aktivät und ihre Beziehungeb zu Fruchtbarkeitsmerkmalen einger Ackerbö unter besonderer Berücksichtigung der Dehydrogenaseaktivät.2. Mitt Zentralbl Bakt II 124:24–36

    Google Scholar 

  • Thalmann A (1968) Zur Methodik der Bestimmung der Dehydrogenaseaktivät im Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtsch Forsch 21:249–258

    CAS  Google Scholar 

References

  • Mersi von W, Schinner F (1991) An improved and accurate method of determining the dehydrogenase actitvity of soil with iodonitrotetrazolium chloride. Biol Fertil Soil 11: 216 – 220

    Article  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Öhlinger, H., Von Mersi, W. (1996). Enzymes Involved in Intracellular Metabolism. In: Schinner, F., Öhlinger, R., Kandeler, E., Margesin, R. (eds) Methods in Soil Biology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60966-4_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-60966-4_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64633-1

  • Online ISBN: 978-3-642-60966-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics