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Coleus spp.: In Vitro Culture and the Production of Forskolin and Rosmarinic Acid

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Medicinal and Aromatic Plants VI

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 26))

Abstract

More than 300 species belong to the genus Coleus, a member of the family Lamiaceae. Coleus species are native to tropical and subtropical regions of Africa, Australia, the East Indies, the Malay Archipelago, and the Philippines Lebowitz 1985. Some species, especially those with showy colorful foliage, are grown as ornamentals all over the world. In India, tubers of some Coleus species, namely, C. tuberosus and C.forskohlii, are eaten as vegetables and pickles, leaves of other Coleus species (e.g. C. amboinicus) are used as spices Schultze-Motel 1986. Preparations from several Coleus species are used in Ayurvedic medicine in India, e.g., preparations from C. amboinicus are active against skin problems and worms de Souza and Shah 1988. Other preparations from Coleus are traditionally used against heart diseases, abdominal colic, respiratory disorders, painful micturition, insomnia, and convulsions Ammon and Müller 1985. The genus Coleus was first described by de Loureiro in 1790. The name Coleus is derived from the Greek work koleos, which means sheath. This relates to a typical characteristic of Coleus, where the four filaments fuse at the bottom to form a sheath around the style de Loureiro 1790.

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References

  • Adzet T, Canigueral S, Iglesias J (1988a) A chromatographic survey of polyphenols from Salvia species. Biochem Syst Ecol 16:29–32

    Article  Google Scholar 

  • Adzet T, Vila R, Canigueral S (1988b) Chromatographic analysis of polyphenols of some Iberian Thymus. J Ethnopharmacol 24:147–154

    Article  PubMed  CAS  Google Scholar 

  • Agarwal KC, Parks RE Jr (1982) Synergistic inhibition of platelet aggregation by forskolin plus PGE1 or 2-fluoroadenosine: effects of 2′,5′-dideoxyadenosine and 5-methylthioadenosine. Biochem Pharmacol 31:3713–3716

    Article  PubMed  CAS  Google Scholar 

  • Agarwal KC, Parks RE Jr (1983) Forskolin: a potential antimetastatic agent. Int J Cancer 32:801–804

    Article  PubMed  CAS  Google Scholar 

  • Ahmed B, Merotra R (1991) Coleoside-B: a new phenolic glycoside from Coleus forskohlii. Pharmazie 46:157–158

    CAS  Google Scholar 

  • Ahmed B, Vishwakarma RA (1988) Coleoside, a monoterpene glycoside from Coleus forskolii. Phytochemistry 27:3309–3310

    Article  CAS  Google Scholar 

  • Albert BM (1989) Versuche zur Synthese der Rosmarinsäure. Doctoral Thesis, University of Saarbrücken

    Google Scholar 

  • Ammon HPT, Müller AB (1985) Forskolin: from an Ayurvedic remedy to a modern agent. Planta Med 51:473–477

    Article  PubMed  CAS  Google Scholar 

  • Banthorpe DV, Bilyard HJ, Brown GD (1989) Enol esters of caffeic acid in several genera of the Labiatae. Phytochemistry 28:2109–2113

    Article  CAS  Google Scholar 

  • Baraldi PG, Barco A, Benetti S, Pollini GP, Polo E, Simoni D (1986) The intramolecular nitrile oxide route to forskolin. J Am Soc Chem Commun 1986:757–758

    Article  Google Scholar 

  • Bentham G (1832-1836) VIII. Coleus. In: Labiatarum genera et species. Ridgway London, pp 47–59

    Google Scholar 

  • Bhat SV, Bajwa BS, Dornauer H, de Souza NJ, Fehlhaber HW (1977) Structures and stereochemistry of new labdane diterpenoids from Coleus forskohlii Briq. Tetrahedron Lett 19:1669–1672

    Article  Google Scholar 

  • Blume KL (1826) Bijdragen tot de flora van Nederlandsch Indie. Lands Drukkerij, Batavia

    Google Scholar 

  • Bristow MR, Ginsburg R, Strosberg A, Montgomery W, Minobe W (1984) Pharmacology and inotropic potential of forskolin in the human heart. J Clin Invest 74:212–223

    Article  PubMed  CAS  Google Scholar 

  • Brown R (1810) Prodomus florae novae Hollandiae et insulae van-diemen. Johnson, London

    Google Scholar 

  • Bruka JF (1983) Effects of selected bronchodilators on antigen-and A23187-induced contraction of guinea pig trachea. J Pharmacol Exp Ther 225:427–435

    Google Scholar 

  • Bult H, Herman AG, Rampart M (1985) Modification of endotoxin-induced haemodynamic and haematological changes in the rabbit by methylprednisolone, F (ab’)2 fragments and rosmarinic acid. Br J Pharmacol 84:317–327

    Article  PubMed  CAS  Google Scholar 

  • Burstein NR, Sears ML, Mead A (1984) Aqueous flow in human eyes is reduced by forskolin, a potent adenylate cyclase activator. Exp Eye Res 39:745–749

    Article  PubMed  CAS  Google Scholar 

  • Caprioli J, Sears M (1983) Forskolin lowers intraocular pressure in rabbits, monkeys, and man. Lancet 30:958–960

    Article  Google Scholar 

  • Chaprin N, Ellis BE (1984) Microspectrophotometric evaluation of rosmarinic acid accumulation in single cultured plant cells. Can J Bot 62:2278–2282

    Article  CAS  Google Scholar 

  • Corey EJ, Da Silva Jardine P, Rohloff JC (1988) Total synthesis of (±)-forskolin. J Am Chem Soc 110:3672–3673

    Article  CAS  Google Scholar 

  • De Eknamkul W, Ellis BE (1984) Rosmarinic acid production and growth characteristics of Anchusa officinalis cell suspension cultures. Planta Med 51:346–350

    Article  Google Scholar 

  • De Eknamkul W, Ellis BE (1985a) Effects of macronutrients on growth and rosmarinic acid formation in cell suspension cultures of Anchusa officinalis. Plant Cell Rep 4:46–49

    Article  Google Scholar 

  • De Eknamkul W, Ellis BE (1985b) Effects of auxins and cytokinins on growth and rosmarinic acid formation in cell suspension cultures of Anchusa officinalis. Plant Cell Rep 4:50–53

    Article  Google Scholar 

  • De Eknamkul W, Ellis BE (1987a) Tyrosine aminotransferase: the entrypoint enzyme of the tyrosine-derived pathway in rosmarinic acid biosynthesis. Phytochemistry 26:1941–1946

    Google Scholar 

  • De Eknamkul W, Ellis BE (1987b) Purification and characterization of tyrosine aminotransferase activities from Anchusa officinalis cell cultures. Arch Biochem Biophys 257:430–438

    Article  Google Scholar 

  • De Eknamkul W, Ellis BE (1988) Rosmarinic acid: production in plant cell cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 310–329

    Google Scholar 

  • De Loureiro J (1790) Flora Cochinchinensis, vol. 2. Academiae Ulyssipone, Lisbon, p 272

    Google Scholar 

  • Delpech B, Lett R (1987) Retrosynthetic studies with forskolin. Tetrahedron Lett 28:4061–4064

    Article  CAS  Google Scholar 

  • De Souza NJ, Shah V (1988) Forskolin-an adenylate cyclase activating drug from an Indian herb. In: Wagner H, Hikino H, Farnsworth NR (eds) Economic and medicinal plant research, vol 2. Academic Press, London, pp 1–16

    Google Scholar 

  • De Tommasi N, De Simone F, De Feco V, Pizza C (1991) Phenylpropanoid glycosides and rosmarinic acid from Momordica balsamina. Planta Med 57:201

    Article  PubMed  Google Scholar 

  • Dohadwalla AN (1986) Biological activities of forskolin. In: Rupp RH, de Souza NJ, Dohadwalla AN (eds) Forskolin: its chemical, biological and medical potential. Hoechst India, Bombay, pp 19–30

    Google Scholar 

  • Dorner WG (1985) Die Melisse — immer noch zu Überraschungen fähig. Pharm. Unserer Zeit 14:112–121

    Article  PubMed  CAS  Google Scholar 

  • Dubey MP, Srimal RC, Nityanand S, Dhawan BN (1981) Pharmacological studies on coleonol, a hypotensive diterpene from Coleus forskohlii. J Ethnopharmacol 3:1–13

    Article  PubMed  CAS  Google Scholar 

  • Ellis BE (1985) Characterization of clonal cultures of Anchusa officinalis derived from single cells of known productivity. J Plant Physiol 119:149–158

    Article  CAS  Google Scholar 

  • Ellis BE, Towers GHN (1970) Biogenesis of rosmarinic acid in Mentha. Biochem J 118:291–297

    PubMed  CAS  Google Scholar 

  • Ellis BE, Remmen S, Goeree G (1979) Interactions between parallel pathways during biosynthesis of rosmarinic acid in cell suspension cultures of Coleus blumei. Planta 147:163–167

    Article  CAS  Google Scholar 

  • Englberger W, Hadding U, Etschenberg E, Graf E, Leyck S, Winkelmann J, Parnham MJ (1988) Rosmarinic acid: a new inhibitor of complement C3-convertase with anti-inflammatory activity. Int J Immunopharmacol 10:729–737

    Article  PubMed  CAS  Google Scholar 

  • Everett TH (ed) (1981) The New York Botanical Garden Illustrated. Encyclopedia of Horticulture, vol 3, Garland, New York, pp 824–827

    Google Scholar 

  • Fukui H, Yazaki K, Tabata M (1984) Two phenolic acids from Lithospermum erythrorhizon cell suspension cultures. Phytochemistry 23:2398–2399

    Article  CAS  Google Scholar 

  • Gamborg OL, Eveleigh DE (1968) Culture methods and detection of glucanases in suspension cultures of wheat and barley. Can J Biochem 46:417–421

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Ganguli BN (1986) Microbial transformation of forskolin derivatives. In: Rupp RH, de Souza NJ, Dohadwalla AN (eds) Forskolin: its chemical, biological and medical potential. Hoechst India, Bombay, pp 31–38

    Google Scholar 

  • Gardener A (1855) Coleus blumei. Florist 8:285–286

    Google Scholar 

  • Gertlowski C (1991) Einfluss des Kulturmediums auf die Produktion von Rosmarinsäure in Suspensionskulturen von Coleus blumei. Diploma Thesis, University of Düsseldorf

    Google Scholar 

  • Gertlowski C, Petersen M (1993) Influence of the carbon source on growth and rosmarinic acid production in suspension cultures of Coleus blumei. Plant Cell, Tissue Org Cult 34:183–190

    Article  CAS  Google Scholar 

  • Gracza L, Ruff P (1984) Rosmarinsäure in Arzneibuchdrogen und ihre HPLC-Bestimmung. Arch Pharm 317:339–345

    Article  CAS  Google Scholar 

  • Gracza L, Koch H, Löffler E (1985) Isolierung von Rosmarinsäure aus Symphytum officinale und ihre anti-inflammatorische Wirksamkeit in einem in vitro-Modell. Arch Pharm 318:1090–1095

    Article  CAS  Google Scholar 

  • Häusler E, Petersen M, Alfermann AW (1991) Hydroxyphenylpyruvate reductase from cell suspension cultures of Coleus blumei Benth. Z Naturforsch 46c: 371–376

    Google Scholar 

  • Häusler E, Petersen M, Alfermann AW (1993) Isolation of protoplasts and vacuoles from cell suspension cultures of Coleus blumei Benth. Plant Cell Rep 12:510–512

    Article  Google Scholar 

  • Hanefeld M, Herrmann K (1976) Quantitative determination of caffeic acid esters and catechins by direct measurement on thin-layer chromatograms. J Chromatogr 123:391–395

    Article  CAS  Google Scholar 

  • Harborne JB (1966) Caffeic acid ester distribution in higher plants. Z Naturforsch 21b: 604–605

    Google Scholar 

  • Hashimoto S, Sakata S, Sonegawa M, Ikegami S (1988) A total synthesis of ( ± )-forskolin. J Am Chem Soc 110:3670–3672

    Article  CAS  Google Scholar 

  • Heller R (1953) Recherches sur la nutrition minérale des tissus végétaux cultivés in vitro. Ann Sci Nat Bot Biol Veg 14:1–223

    Google Scholar 

  • Hiller K (1965) Zur Kenntnis der Inhaltsstoffe einiger Saniculoideae. 1. Mitteilung: Sanicula europaea L. Isolierung und quantitative Erfassung von Chlorogen-und Rosmarinsäure. Pharmazie 20:574–579

    CAS  Google Scholar 

  • Hippolyte I, Marin B, Baccou JC, Jonard R (1991) Influence du milieu d’entretien et de la concentration en saccharose sur la production d’acide rosmarinique par des suspensions cellulaires de sauge (Salvia officinalis L.). CR Acad Sci Paris 313 Ser III: 365–371

    Google Scholar 

  • Inamdar PK, Dornauer H, de Souza NJ (1980) GLC method for assay of forskolin, a novel positive inotropic and blood pressure-lowering agent. J Pharm Sci 69: 1449–1451

    Article  PubMed  CAS  Google Scholar 

  • Inamdar PK, Kanitkar PV, Reden J, de Souza NJ (1984) Quantitative determination of forskolin by TLC and HPLC. Planta Med 50:30–34

    Article  PubMed  CAS  Google Scholar 

  • Inamdar PK, Khandelwal Y, Garkhedkar M, Rupp RH, de Souza NJ (1989) Identification of microbial transformation products of 1,9-dideoxyforskolin and 7-deacetyl-1,9-dideoxyforskolin. Planta Med 55:386–387

    Article  PubMed  CAS  Google Scholar 

  • Jenkins PR, Menear KA, Barraclough P, Nobbs MS (1984) An intramolecular Diels-Alder approach to forskolin. J Chem Soc Chem Commun 1984:1423–1424

    Article  Google Scholar 

  • Karwatzki B, Petersen M, Alfermann AW (1989) Transient activity of enzymes involved in the biosynthesis of rosmarinic acid in cell suspension cultures of Coleus blumei. Planta Med 55:663–664

    Article  Google Scholar 

  • Kimura Y, Okuda H, Okuda T, Hatano T, Arichi S (1987) Studies on the activities of tannins and related compounds, X. Effects of caffeetannins and related compounds on arachidonate metabolism in human polymorphonuclear leukocytes. J Nat Prod 50:392–399

    CAS  Google Scholar 

  • Krombholz R, Mersinger R, Kreis W, Reinhard E (1992) Production of forskolin by axenic Coleus forskohlii roots cutivated in shake flasks and 20-1 glass jar bioreactors. Planta Med 58: 328–333

    Article  PubMed  CAS  Google Scholar 

  • Lebowitz RJ (1985) The genetics and breeding of Coleus. Plant Breed Rev 3:343–360

    Google Scholar 

  • Lichey J, Friedrich T, Priesnitz M, Biamino G, Usinger P, Huckauf H (1984) Effect of forskolin on methacholine-induced bronchoconstriction in extrinsic asthmatics. Lancet 21:167

    Article  Google Scholar 

  • Lindner E, Dohadwalla AN, Bhattacharya BK (1978) Positive inotropic and blood pressure lowering activity of a diterpene derivative isolated from Coleus forskohlii: Forskolin. Drug Res 28:284–289

    CAS  Google Scholar 

  • Linnaeus C (1763) Species Plantarum. Impensis Direct. Laurentii Salvii, Stockholm

    Google Scholar 

  • Mandler-Henger A (1988) Somaclonale Variation bei Coleus forskohlii. Doctoral Thesis, iversity of Tübingen

    Google Scholar 

  • Mersinger R (1988) Bildung von Forskolin und verwandten Labdan-Diterpenen durch Zellkulturen von Coleus forskohlii. Doctoral Thesis, University of Tübingen

    Google Scholar 

  • Mersinger R, Dornauer H, Reinhard E (1988) Formation of forskolin by suspension cultures of Coleus forskohlii. Planta Med 54:200–204

    Article  PubMed  CAS  Google Scholar 

  • Mizukami H, Ellis BE (1991) Rosmarinic acid formation and differential expression of tyrosine aminotransferase isoforms in Anchusa officinalis cell suspension cultures. Plant Cell Rep 10:321–324

    Article  CAS  Google Scholar 

  • Mølgaard P, Ravn H (1988) Evolutionary aspects of caffeoyl ester distribution in dicotyledons. Phytochemistry 27:2411–2421

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nadkarni SR, Akut PM, Ganguli BN, Khandelwal Y, de Souza NJ, Rupp RH, Fehlhaber H (1986) Microbial transformation of 1,9-dideoxyforskolin to forskolin. Tetrahedron Lett 27:5265–5268

    Article  CAS  Google Scholar 

  • Nicolau KC, Li WS (1985) An intramolecular Diels-Alder strategy to forskolin. J Chem Soc Chem Commun 1985:421

    Article  Google Scholar 

  • Oplinger JA, Paquette LA (1987) Synthesis of the forskolin skeleton via anionic oxy-cope rearrangement. Tetrahedron Lett 28:5441–5444

    Article  CAS  Google Scholar 

  • Pabsch K, Petersen M, Rao NN, Alfermann AW, Wandrey C (1991) Chemo-enzymatic synthesis of rosmarinic acid. Recl Trav Chim Pays-Bas 110:199–205

    Article  CAS  Google Scholar 

  • Parnham MJ, Kesselring K (1985) Rosmarinic acid. Drugs Future 10:756–757

    Google Scholar 

  • Paulus EF (1980a) Molecular and crystal structure of forskolin. Z Kristallogr. 152:239–245

    Article  CAS  Google Scholar 

  • Paulus EF (1980b) Molecular and crystal structure of 1-benzyl-7-desacetyl-7-bromoisobutyryl-forskolin. Absolute configuration of forskolin. Z Kristallogr 153:43–49

    CAS  Google Scholar 

  • Petersen MS (1991) Characterization of rosmarinic acid synthase from cell cultures of Coleus blumei. Phytochemistry 30:2877–2881

    Article  CAS  Google Scholar 

  • Petersen M (1993) Purification of rosmarinic acid synthase from cell cultures of Coleus blumei. Planta 191:18–22

    Article  CAS  Google Scholar 

  • Petersen M, Alfermann AW (1988) Two new enzymes of rosmarinic acid biosynthesis from cell cultures of Coleus blumei: hydroxyphenylpyruvate reductase and rosmarinic acid synthase. Z Naturforsch 43c: 501–504

    Google Scholar 

  • Petersen M, Häusler E, Karwatzki B, Meinhard J (1993) Proposed biosynthetic pathway for rosmarinic acid in cell cultures of Coleus blumei. Planta 189:10–14

    Article  CAS  Google Scholar 

  • Rampart M, Beetens JR, Bult H, Herman AG, Parnham MJ, Winkelmann J (1986) Complement-dependent stimulation of prostacyclin biosynthesis: inhibition by rosmarinic acid. Biochem Pharmacol 35:1397–1400

    Article  PubMed  CAS  Google Scholar 

  • Razzaque A, Ellis BE (1977) Rosmarinic acid production in Coleus cultures. Planta 137:287–291

    Article  CAS  Google Scholar 

  • Reschke A (1983) Capillargaschromatographische Bestimmung der Rosmarinsäure in Blattgewürzen. Z Lebensm Unters Forsch 176:116–119

    Article  CAS  Google Scholar 

  • Reuff I, Seitz U, Ulbrich B, Reinhard E (1988) Cryopreservation of Coleus blumei suspension and callus cultures. J Plant Physiol 133:414–418

    Article  Google Scholar 

  • Saksena AK, Green MJ, Shue HJ, Wong JK, McPhail AT (1985) Identity of coleonol with forskolin: structure revision of a base-catalysed rearrangement product. Tetrahedron Lett 26:551–554

    Article  CAS  Google Scholar 

  • Scarpati ML, Oriente G (1958) Isolamento e costituzione dell’ acido rosmarinico (dal Rosmarinus off). Ric Sci 28:2329–2333

    CAS  Google Scholar 

  • Scarpati ML, Oriente G (1960) Costituzione stereochimica dell acido β(3,4-diossifenil) α-lattico dal Rosmarinus off. Ric Sci 30:255–259

    CAS  Google Scholar 

  • Schultze-Motel J (ed) (1986) Rudolf Mansfelds Verzeichnis landwirtschaftlicher und gärtnerischer Kulturpflanzen (ohne Zierpflanzen), Band 3. Akademie-Verlag, Berlin

    Google Scholar 

  • Sen J, Sharma AK (1991) In vitro propagation of Coleus forskohlii Briq. for forskolin synthesis. Plant Cell Rep 9:696–698

    CAS  Google Scholar 

  • Sen J, Sharma AK, Sahu NP, Mahato SB (1992) Production of forskolin in in vitro cultures of Coleus forskohlii Briq. Planta Med 58:324–327

    Article  PubMed  CAS  Google Scholar 

  • Shah V, Bhat SV, Baiwa BS, Dornauer H, de Souza NJ (1980) The occurrence of forskolin in the Labiatae. Planta Med 39:183–185

    Article  CAS  Google Scholar 

  • Sharma N, Chandel KPS, Srivastava VK (1991) In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant. Plant Cell Rep 10:67–70

    Article  CAS  Google Scholar 

  • Siebert A, Voss A (1896) Vilmorin’s Blumengärtnerei. Paul Parey, Berlin

    Google Scholar 

  • Sumaryono W, Proksch P, Hartmann T, Nimtz M, Wray V (1991a) Induction of rosmarinic acid accumulation in cell suspension cultures of Orthosiphon aristatus after treatment with yeast extract. Phytochemistry 30:3267–3271

    CAS  Google Scholar 

  • Sumaryono W, Proksch P, Wray V, Witte L, Hartmann T (1991b) Qualitative and quantitative analysis of the phenolic constituents from Orthosiphon aristatus. Planta Med 57:176–180

    Article  PubMed  CAS  Google Scholar 

  • Takeda R, Hasegawa J, Sinozaki K (1990) Phenolic compounds from Anthocerotae. In: Zinsmeister HD, Mues R (eds) Bryophytes. Their chemistry and chemical taxonomy. Proc Phytochemical Society of Europe, vol 29. Oxford Science Publications, Clarendon Press, Oxford, pp 201–207

    Google Scholar 

  • Tandon JS, Dhar MM, Ramakumar S, Venkatesan K (1977) Structure of coleonol, a biologically active diterpene from Coleus forskohlii. Indian J Chem 15B: 880–883

    CAS  Google Scholar 

  • Ulbrich B (1986) Nutrition and environment of plant cells in bioreactors. In: Korhola M, Tuompo H, Kauppinen V (eds) Proc Conf on Global Impacts of Applied Microbiology: Symposia on Alcohol Fermentation and Plant Cell Culture, Helsinki 1985, vol 4, Foundation for Biotechnical and Industrial Fermentation Research, Helsinki, pp 147–164

    Google Scholar 

  • Ulbrich B, Wiesner W, Arens H (1985) Large-scale production of rosmarinic acid from plant cell cultures of Coleus blumei Benth. In: Neumann KH, Barz W, Reinhard E (eds) Primary and secondary metabolism of plant cell cultures. Springer, Berlin Heidelberg New York, pp 293–303

    Chapter  Google Scholar 

  • Valdés III. LJ, Mislankar SG, Paul AG (1987) Coleus barbatus (C. forskohlii (Lamiaceae) and the potential new drug forskolin (coleonol). Econ Bot 41:474–483

    Article  Google Scholar 

  • Verotta L (1985) Isolation and HPLC determination of the active principle of Rosmarinus officinalis and Gentiana lutea. Fitoterapia 56:25–29

    CAS  Google Scholar 

  • Vishwakarma RA, Tyagi BR, Ahmed B, Husain A (1988) Variation in forskolin content in the roots of Coleus forskohlii. Planta Med 54:471–472

    Article  PubMed  CAS  Google Scholar 

  • Whitaker RJ, Hashimoto T, Evans DA (1984) Production of the secondary metabolite, rosmarinic acid, by plant cell suspension cultures. Ann NY Acad Sci 435:364–366

    Article  CAS  Google Scholar 

  • White PR (1963) The cultivation of animal and plant cells. The Ronald Press, New York

    Google Scholar 

  • Winterhoff H, Gumbinger HG, Sourgens H (1988) On the antigonadotropic activity of Lithospermum and Lycopus species and some of their phenolic constituents. Planta Med 54:101–106

    Article  PubMed  CAS  Google Scholar 

  • Zenk MH, El-Shagi H, Ulbrich B (1977) Production of rosmarinic acid by cell-suspension cultures of Coleus blumei. Naturwissenschaften 64:585–586

    Article  CAS  Google Scholar 

  • Ziegler FE, Jaynes BH, Saindane MT (1985) A C6C7 oxygen functionalized intermediate for the synthesis of forskolin: stereochemical control in an intermolecular Diels-Alder reaction. Tetrahedron Lett 26:3307–3310

    Article  CAS  Google Scholar 

  • Ziegler FE, Jaynes BH, Saindane MT (1987) A synthetic route to forskolin. J Am Chem Soc 109:8115–8116

    Article  CAS  Google Scholar 

  • Zinsmeister HD, Becker H, Eicher T (1991) Moose, eine Quelle biologisch aktiver Naturstoffe? Angew Chem 103:134–151

    Article  CAS  Google Scholar 

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Petersen, M. (1994). Coleus spp.: In Vitro Culture and the Production of Forskolin and Rosmarinic Acid. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants VI. Biotechnology in Agriculture and Forestry, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57970-7_5

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