Abstract
The electrochemical approach coupled with Sb microelectrode was developed to determine the carbonic anhydrase activity in a wide range. The sensing response of the microelectrode had a good linear relationship between potential and pH value in barbital buffer. The temperature would not affect the linear relationship. During the determination, the open-circuit potential method was taken to monitor the whole course of the reversible conversion catalyzed by carbonic anhydrase, and then the initial part of uniform velocity from the reaction curve was chosen to calculate the reaction velocity (the time to change one unit of pH). This technique, in comparison with the conventional method was used to determine the activities of bovine red blood cells carbonic anhydrase, foliar carbonic anhydrase and extracellular carbonic anhydrase in some plants. The result showed that the electrochemical approach coupled with Sb microelectrode would obtain more credible, accurate data than the conventional method.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
References
Lindskog, S.: Structure and mechanism of carbonic anhydrase. Pharmacol. Ther. 74, 1–20 (1997)
Nimer, N.A., Brownlee, C., Merrett, M.J.: Extracellular carbonic anhydrase facilitates carbon dioxide availability for photosynthesis in the marine Dinoflagellate Prorocentrummicans. Plant Physiol. 120, 105–112 (1999)
Wilbur, K.M., Anderson, N.G.: Electrometric and colorimetric determination of carbonic anhydrase. J. Biol. Chem. 176, 147–154 (1948)
Yao, S., Wang, M., Madou, M.: A pH electrode based on melt-oxides iridum oxide. J. Electrochem. Soc. 36, H29–H36 (2001)
Li, Q.W., Luo, G.A., Shu, Y.Q.: Response of nanosized cobalt oxide electrodes as pH sensors. Anal. Chim. Acta 409, 137–142 (2000)
Chen, D.C., Lai, Y.T., Li, W.F.: Study of the H + response behavior of WO3 based pH electrochemical sensor. Micronanoelectronic. Tech. 7, 397–399 (2007)
Wang, M., Yao, S.: Carbonate-melt oxide iridium wire for pH sensing. Electroanal. 15, 1606–1615 (2003)
Imokawa, T., Williams, K.J., Denuault, G.: Fabrication and characterization of nanostructured Pd hydride pH microelectrodes. Anal. Chem. 78, 265–271 (2006)
Moroney, J.V., Husic, H.D., Tolbert, N.E.: Effect of carbonic anhydrase inhibitors on inorganic carbon accumulation by Chlamydomonas reinhardtii.. Plant Physio. 79, 177–183 (1985)
Arnold, D.C., Tinker, M.A.: The fixational pause of the eyes. J. Exp. Psycho. 25, 271–280 (1939)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Wu, Y. et al. (2011). An Electrochemical Approach Coupled with Sb Microelectrode to Determine the Activities of Carbonic Anhydrase in the Plant Leaves. In: Zeng, D. (eds) Future Intelligent Information Systems. Lecture Notes in Electrical Engineering, vol 86. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19706-2_12
Download citation
DOI: https://doi.org/10.1007/978-3-642-19706-2_12
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-19705-5
Online ISBN: 978-3-642-19706-2
eBook Packages: EngineeringEngineering (R0)