This chapter focuses on showing the biotechnological methods on fungal inoculum and mycorrhizal plant production, developed to cultivate, for the first time, some species of the Terfezia genus. The mycorrhizal synthesis between desert truffles and the Helianthemum species is carried out in different ways according to the type of fungal inoculum (spores or mycelium), the plant source (seedlings or micropropagated plantlets) and the culture conditions (in vivo or in vitro) used. The different options are designed to obtain a continuous desert truffle mycorrhizal plant production and to also avoid any limiting factor in this production. Moreover, we have developed a very simple management plantation protocol, after the experience acquired over the years, and after reducing the fructification time from the plantation from 23 months to 12 months. It consists of mainly planting in spring, a single irrigation during the dry summer and a yearly weeding after the third year of plantation.
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Morte, A., Honrubia, M., Gutiérrez, A. (2008). Biotechnology and Cultivation of Desert Truffles. In: Varma, A. (eds) Mycorrhiza. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-78826-3_23
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