Abstract
The type of thein vitro root interactions ofTerfezia terfezioides with the plantsRobinia pseudoacacia andHelianthemum ovatum was investigated including detailed anatomical and ultrastructural characterization. No difference in growth was detected at different phosphate concentrations on agar synthetic medium between the inoculated and control plants during a short-time cultivation. The fungal colonization of the roots increased with higher phosphate level in both plant species, but was always lower inR. pseudoacacia roots. Septate hyphae formed frequently intracellular branched coils in dead cortical cells. InH. ovatum intercellular hyphae were observed forming finger-like structures reminiscent of Hartig-net structures in ectomycorrhizae. A loose hyphal envelope covered the root surface of both colonized and noncolonized roots. The features resembled similar structures described earlier during the mycorrhizae of differentTerfezia species. Our detailed anatomical and ultrastructural study shows that thein vitro root interactions of theT. terfezioides cannot be considered unambiguously as mycorrhiza.
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The works was partly conducted during the stay of the first author at theLehrstuhl Spezielle Botanik/Mykologie (Universität Tübingen) with the support of theMinisterium für Wissenschaft, Forschung und Kunst Baden-Württemberg (Germany).
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Kovács, G.M., Vágvölgyi, C. & Oberwinkler, F. In Vitro interaction of the truffleTerfezia terfezioides withRobinia pseudoacacia andHelianthemum ovatum. Folia Microbiol 48, 369–378 (2003). https://doi.org/10.1007/BF02931369
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DOI: https://doi.org/10.1007/BF02931369