Overview
- Identifies the proper use of each component of immunohistochemical experiments
- Concise and intuitive guide to using immunohistochemical techniques
- Technique most useful for the following types of experiments: producing transgenic animals, studying the effects of drug treatments, electrophysiological studies, isolating and differentiating stem cells, super-resolution microscopy
- Includes supplementary material: sn.pub/extras
Part of the book series: Techniques in Life Science and Biomedicine for the Non-Expert (TLSBNE)
Buy print copy
About this book
Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments:
· producing transgenic animals
· studying the effects of drug treatments
· electrophysiological studies
· animal surgery· isolating and differentiating stemcells
· super-resolution microscopy
Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.Similar content being viewed by others
Keywords
Table of contents (9 chapters)
-
Front Matter
-
Back Matter
Authors and Affiliations
About the author
Alexander E. Kalyuzhny graduated in 1981 from the Biological Department of the Saratov State University in Russia. After graduation, he performed research on the effects of narcotic drugs on the morphogenesis of early embryos, which he defended as his Ph.D. thesis in 1987. In 1992, Dr. Kalyuzhny was invited to join the Department of Pharmacology at the University of Minnesota (USA), where he is currently affiliated with the Department of Neuroscience. He is the author of 31 papers in peer-reviewed journals and 16 book chapters, and has served as the editor of 3 books (Humana Press/Springer). He is also the editor-in-chief of the open-access journal Cells.
Bibliographic Information
Book Title: Immunohistochemistry
Book Subtitle: Essential Elements and Beyond
Authors: Alexander E. Kalyuzhny
Series Title: Techniques in Life Science and Biomedicine for the Non-Expert
DOI: https://doi.org/10.1007/978-3-319-30893-7
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer International Publishing Switzerland 2016
Hardcover ISBN: 978-3-319-30891-3Published: 06 April 2016
Softcover ISBN: 978-3-319-80910-6Published: 25 April 2018
eBook ISBN: 978-3-319-30893-7Published: 24 March 2016
Series ISSN: 2367-1114
Series E-ISSN: 2367-1122
Edition Number: 1
Number of Pages: XIV, 85
Number of Illustrations: 34 b/w illustrations, 2 illustrations in colour
Topics: Molecular Medicine, Antibodies, Neurochemistry