Abstract
Primary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital.
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Acknowledgements
We thank Dr. Rick Moore (NIEHS, North Carolina, USA) for advice. L.H. is a recipient of a Drummond Scholarship.
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© 2013 Springer Science+Business Media New York
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Edwards, M., Houseman, L., Phillips, I.R., Shephard, E.A. (2013). Isolation of Mouse Hepatocytes. In: Phillips, I., Shephard, E., Ortiz de Montellano, P. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology, vol 987. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-321-3_24
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DOI: https://doi.org/10.1007/978-1-62703-321-3_24
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-320-6
Online ISBN: 978-1-62703-321-3
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