Abstract
In this chapter, the isolation of primary mouse hepatocytes and their response to chemical treatment are described. We show that it is important to consider, in the experimental design, the sex of the animals to be used. We demonstrate this by measuring the effect of sex hormones or xenobiotics on the expression of flavin-containing monooxygenase 5 in cultures of primary hepatocytes isolated from male and female mice.
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Acknowledgements
We thank Dr. Rick Moore (NIEHS, North Carolina, United States of America) for advice. Lyndsey Houseman was a recipient of a Drummond Scholarship.
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Houseman, L., Edwards, M., Phillips, I.R., Shephard, E.A. (2015). Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments. In: Vinken, M., Rogiers, V. (eds) Protocols in In Vitro Hepatocyte Research. Methods in Molecular Biology, vol 1250. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2074-7_1
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DOI: https://doi.org/10.1007/978-1-4939-2074-7_1
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2074-7
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