Abstract
Due to their special structural features, extraction and isolation of saponins poses a serious challenge. Conventional methods have been explored as well as the recent, relatively greener, efficient, solvent-economic, time-saving, newer methods of extraction. Both traditional and recent methods of isolation are also discussed. Finally, examples are given involving both conventional and newer methods of extraction and isolation. Though in general it is difficult to use a single technique for isolation of saponins, recent literature work seems to point to the fact that high speed counter-current separation coupled to evaporative light scattering detector (ELSD) gives superior separation. The ELSD appears to have circumvented the long-standing problem of saponin detection as most of these do not have a chromophore, and hence making UV detection only nonspecific and at range 200–210 nm.
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References
Hostettmann K, Marston A (1995) Saponins. Cambridge University Press, Cambridge
Çalis I, Yusofoglu H, Zerbe O, Sticher O (1999) Cephalotoside A: a tridesmosidic cycloartane type glycoside from Astralagus cephalotes var. brevecalys. Phytochemistry 50:843–847
Yesilada E, Bedir E, Çalis I, Takaishi Y, Ohmoto Y (2005) Effects of triterpene saponins from Astralagus species on in vitro cytokine release. J Ethnopharmacol 96:71–77
Semmar N, Tomofumi M, Mrabet Y, Lacaille-Dubois M-A (2010) Two new acylated tridesmosidic saponins from Astralagus armatus. Helv Acta Chim 93:871–876
Kuljanabhagavad T, Wink M (2009) Biological activities and chemistry of saponins from Chenopodium quinoa Willd. Phytochem Rev 8:473–490
Inose Y, Miyase T, Ueno A (1992) Studies on constituents of Solidago virga-aurea L. II. Structures of solidagosaponins. Chem Pharm Bull 40:946–953
Vincken J-P, Heng L, de Groot A, Gruppen H (2007) Saponins, classification and occurrence in the plant kingdom. Phytochemistry 68:275–276
Oleszek W, Bialy Z (2006) Chromatographic determination of plant saponins – an update (2002–2005). J Chromatogr A 1112:78–91
Li B, Abliz Z, Tang M, Fu G, Yu S (2006) Rapid structural characterization of triterpenoid saponins in crude extract from Symplocos chinensis using liquid chromatography combined with electrospray ionization tandem mass spectrometry. J Chromatogr A 1101:53–62
Ha YW, Lim SS, Ha IN, Na Y-C, Seo J-J, Shin H, Son SH, Kim YS (2007) Preparative isolation of four ginsenosides from Korean red gingseng (steam-treated Panax gingseng C. A. Meyer), by high-speed counter-current chromatography coupled with evaporative light scattering detection. J Chromatogr A 1151:37–44
Qi X, Ignatova S, Luo G, Liang Q, Jun FW, Wang Y, Sutherland I (2010) Preparative isolation and purification of Rf, Re, Rd and Rb1 from the roots of Panax gingseng with a salt containing solvent system and flow step-gradient by high performance counter-current chromatography with evaporative light scattering detector. J Chromatogr A 1217:1995–2001
Müller A, Ganzera M, Stuppner H (2005) Analysis of phenolic glycosides and saponins in Primula elatior and Primula veris (primula root) by liquid chromatography, evaporative light scattering detection and mass spectrometry. J Chromatogr A 1112:218–223
Ito Y, Conway WD (1996) High speed counter-current chromatography. Wiley-Interscience, New York
Du Q, Cheng H, Ito Y (2001) Separation of radioactive metabolites in cultured tea cells fed with [14C]phenylalanine using high-speed counter-current chromatography. J Chromatogr A 921:331–334
Du QZ, Li ZH, Ito Y (2001) Preparative separation of isoflavone components in soybeans using high-speed counter-current chromatography. J Chromatogr A 921:271–274
Du Q, Xia M, Ito Y (2002) Purification of icariin from the extract of Epimedium segittatum using high-speed counter-current chromatography. J Chromatogr A 962:239–241
Du QZ, Chai WJ, Ito Y (2002) Preparative separation of fruit extract of Silybum marianum using a high-speed countercurrent chromatograph with scale-up columns. J Liq Chromatogr Rel Technol 25:2515–2520
Weng A, Jenett-Siems K, Schmieder P, Bachran D, Bachran C, Görick C, Thakur M, Fuchs H, Melzig MF (2010) A convenient method for saponin isolation in tumor therapy. J Chromatogr B 878:713–718
Chen Y, Xie M-Y, Gong X-F (2007) Microwave-assisted extraction used for the isolation of total triterpenoid saponins form Ganoderma atrum. J Food Eng 81:162–170
Kwon JH, Bélanger JMR, Páre JRJ (2003) Optimisation of microwave-assisted extraction (MAE) for gingseng components by response surface methodology. J Agric Food Chem 51:1807–1810
Kwon JH, Bélanger JMR, Páre JRJ, Yaylayan VA (2003) Applications of microwave-assisted process (MAP™) to the fast extraction of ginseng saponins. Food Res Int 36:491–498
Kwon JH, Lee GD, Bélanger JMR, Páre JRJ (2003) Effects of ethanol concentration on the efficiency of extraction of ginseng saponins when using when using a microwave-assisted process (MAP™). Int J Food Sci Tech 38:615–622
Vongsangnak W, Gua J, Chauvatcharin S, Zhong JJ (2004) Towards efficient extraction of notoginseng saponins from cultured cells of Panax notoginseng. Biochem Eng J 18:115–120
Wu J, Lin L, Chau F (2001) Ultrasound-assisted extraction of ginseng spaonins cultured in ginseng cells. Sonochem 8:347–352
Kerem Z, German-Shashoua H, Yaden O (2005) Microwave-assisted extraction of bioactive saponins from chickpea (Cicer arietinum L). J Sci Food Agric 85:406–412
Eskilsson CS, Bjorkland E (2000) Analytical-scale microwave-assisted extraction. J Chromatogr A 902:227–250
Marston A, Wolfender JL, Hostettmann K (2000) Analysis and isolation of saponins from plant material. In: Oleszek WA, Marston A (eds) Saponins in foodstuffs and medicinal plants. Annual proceedings of the phytochemical society, Clarendon, pp 1–12
Oleszek WA (2002) Chromatographic determination of plant saponins. J Chromatogr A 967:147–162
Woldemichael GM, Wink M (2001) Identification and biological activities of triterpenoid saponins from Chenopodium quinoa. J Agric Food Chem 49:2327–2332
Kuljanabhagavad T, Thongphasuk P, Chamulitrat W, Wink M (2008) Triterpene saponins from Chenopodium quinoa Willd. Phytochemistry 69:1919–1926
Schöpke T, Agha MIH, Wray V, Hiller K (1994) Triterpenoid saponins from Bellium bellidioides. Phytochemistry 36:449–453
Schöpke T, Wray V, Nimtz M, Hiller K (1994) Triterpenoid saponins from Bellium bellidioides. Structure of the minor deacylsaponins. Phytochemistry 41:1399–1403
Miyase T, Shiokawa K-I, Zhang DM, Ueno A (1996) Araliasaponin I–XI, triterpene saponins from the roots of Aralia decaisneana. Phytochemistry 41:1411–1418
Du Q, Jerz G, Waibel R, Winterhalter P (2003) Isolation of dammarane saponins from Panax ginseng by high speed counter-current chromatography. J Chromatogr A 1008:173–180
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Majinda, R.R.T. (2012). Extraction and Isolation of Saponins. In: Sarker, S., Nahar, L. (eds) Natural Products Isolation. Methods in Molecular Biology, vol 864. Humana Press. https://doi.org/10.1007/978-1-61779-624-1_16
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DOI: https://doi.org/10.1007/978-1-61779-624-1_16
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