Abstract
Insects are convenient models for assessing the virulence of microbial pathogens or for assessing the efficacy of antimicrobial drugs and give results comparable to those that can be obtained using mammals. Galleria mellonella larvae are easy to purchase and inoculate and provide results within 48 h. Various parameters may be used to monitor the effect of a pathogen on the insect and, as a consequence, measure its relative virulence. Larval death, changes in immune cells (haemocytes) numbers, or the extent of proliferation of the pathogen within the insect haemocoel are good indicators of virulence and of the insect’s immune response. Analysing the humoral immune response also gives insight into the interaction of the pathogen with the insect. Changes in gene expression or the expression of key antimicrobial peptides provide data on this element of the insect’s response and, through extrapolation, how the mammalian immune system might respond. G. mellonella larvae, therefore, provide a quick and convenient means of measuring microbial virulence and are a useful alternative to the use of mammals for this type of screening.
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References
Kavanagh K. and Reeves E.P. (2004). Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens. FEMS Microbiology Reviews. 28: 101–112.
Cotter G., Doyle S. and Kavanagh K. (2000). Development of an insect model for the in vivo pathogenicity testing of yeasts. FEMS Immunol. & Med. Microbiol. 27: 163–169.
Reeves E.P., Messina C.G.M., Doyle S. and Kavanagh K. (2004). Correlation of gliotoxin production and virulence of Aspergillus fumigatus in Galleria mellonella. Mycopathologia 158: 73–79
Brennan M., Thomas D.Y., Whiteway M.,, and Kavanagh K. (2002). Correlation between virulence of Candida albicans mutants in mice and Galleria mellonella larvae. FEMS Immunol. & Med. Microbiol 34: 153–157.
Mylonakis E., Moreno R., El Khoury J.B., Idnurm A., Heitman J., Calderwood S.B., Ausubel F.M. and Diener A. (2005). Galleria mellonella as a model system to study Cryptococcus neoformans pathogenesis. Infect. Immun. 73: 3842–3850.
Lionakis M.S., Lewis R.E., May G.S., Wiederhold N.P., Albert N.D., Halder G., and Kontoyiannis D.P. (2005). Toll-deficient Drosophila flies as a fast, high-throughput model for the study of antifungal drug efficacy against invasive aspergillosis and Aspergillus virulence. J Infect Dis. 191: 1188–95.
Fuchs B. and Mylonakis E. (2006). Using non-mammalian host to study fungal virulence and host defense. Curr. Opin Microbiol. 9: 346–351.
Mylonakis E. (2008). Galleria mellonella and the study of fungal pathogenesis: making the case for another genetically tractable model host. Mycopathol. 165: 1–3.
Bergin D., Brennan M.. and Kavanagh K. (2003). Fluctuations in haemocyte density and microbial load may be used as indicators of fungal pathogenicity in larvae of Galleria mellonella. Microb. Infect. 5: 1389–1395.
Bergin D., Murphy L., Keenan J., Clynes M. and Kavanagh K. (2006). Pre-exposure to yeast protects larvae of Galleria mellonella from a subsequent lethal infection by Candida albicans and is mediated by the increased expression of antimicrobial peptides. Microb. Infect.8: 2105–2112.
Mowlds P., Coates C., Renwick J. and Kavanagh K. (2010). Dose-dependent cellular and humoral responses in Galleria mellonella larvae following β-glucan inoculation. Microb. Infect. 12: 146–153.
Tickoo S., and Russell S. (2002). Drosophila melanogaster as a model system for drug discovery and pathway screening. Curr Opin Pharmacol. 2: 555–60.
Hamamoto H., Kurokawa K., Kaito C., Kamura K., Manitra Razanajatovo I., Kusuhara H., Santa T., and Sekimizu K. (2004). Quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms. Antimicrob. Agent. Chemother. 48: 774–779.
Hamamoto H., Tonioike A., Narushima K., Horie R., and Sekimizu K.(2009). Silkworm as a model animal to evaluate drug candidate toxicity and metabolism. Comp. Biochem. Physiol. 149: 334–339
Rowan R., Moran C., McCann M., and Kavanagh K. (2009). Use of Galleria mellonella larvae to evaluate the in vivo anti-fungal activity of (Ag2(mal)(phen)3). Biometals 22: 461–7.
Cytrnska, M., Mak, P., Zdybicka-Barabas, A., Suder, P., and Jacubowicz T. (2007). Purification and characterization of 8 peptides from Galleria mellonella immune haemolymph. Peptides 28:3: 533–546.
Shevchenko, A., Tomas, H., Havlis,J., Olsen, J.V., and Mann, M. (2006). In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols 1: 2856–2860.
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Fallon, J., Kelly, J., Kavanagh, K. (2012). Galleria mellonella as a Model for Fungal Pathogenicity Testing. In: Brand, A., MacCallum, D. (eds) Host-Fungus Interactions. Methods in Molecular Biology, vol 845. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-539-8_33
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DOI: https://doi.org/10.1007/978-1-61779-539-8_33
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