Abstract
The use of animal models is a key step to better understand bacterial virulence factors and their roles in host/pathogen interactions. To avoid the ethical and cost problems of mammalian models in bacterial virulence research, several insect models have been developed. One of these models, the larvae of the greater wax moth Galleria mellonella, has been shown to be relevant for several fungal and bacterial mammalian pathogens. Here, we describe the use G. mellonella to study virulence of the highly virulent facultative intracellular bacterial pathogens: Brucella suis, Brucella melitensis, Francisella tularensis, Burkholderia mallei, and Burkholderia pseudomallei.
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Abbreviations
- TS:
-
Trypticase soy
- BHI:
-
Brain–heart infusion
- CFU:
-
Colony-forming units
- MOI:
-
Multiplicity of infection
- PBS:
-
Phosphate-buffered saline
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© 2014 Springer Science+Business Media New York
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Sprynski, N., Valade, E., Neulat-Ripoll, F. (2014). Galleria mellonella as an Infection Model for Select Agents. In: Vergunst, A., O'Callaghan, D. (eds) Host-Bacteria Interactions. Methods in Molecular Biology, vol 1197. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1261-2_1
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DOI: https://doi.org/10.1007/978-1-4939-1261-2_1
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1260-5
Online ISBN: 978-1-4939-1261-2
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