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Polyethylene Glycol (PEG)-Mediated Transformation in Filamentous Fungal Pathogens

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Plant Fungal Pathogens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 835))

Abstract

Genetic transformation is an essential tool for the modern study of gene function and the genetic improvement of an organism. The genetic transformation of many fungal species is well established and can be carried out by utilizing different transformation methods including electroporation, Agrobacterium, biolistics, or polyethylene glycol (PEG)-mediated transformation. Due to its technical simplicity and common equipment requirements, PEG-mediated transformation is still the most commonly used method for genetic transformation in filamentous fungi. Here, we describe a PEG-based protocol developed for genetic transformation of Stagonospora nodorum, a fungal pathogen of wheat. This protocol is directly applicable to other fungi especially those in the Dothideomycete class of fungi.

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Author information

Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, USA

    Zhaohui Liu & Timothy L. Friesen

  2. Cereal Crops Research Unit, Northern Crop Science Laboratory, USDA-ARS, Fargo, ND, USA

    Timothy L. Friesen

Authors
  1. Zhaohui Liu
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  2. Timothy L. Friesen
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Corresponding author

Correspondence to Timothy L. Friesen .

Editor information

Editors and Affiliations

  1. Northern Crops Science Laboratory, USDA-ARS, North 18th Street 1307, Fargo, 58105-5677, North Dakota, USA

    Melvin D. Bolton

  2. Lab. Phytopathology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, Netherlands

    Bart P.H.J. Thomma

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Liu, Z., Friesen, T.L. (2012). Polyethylene Glycol (PEG)-Mediated Transformation in Filamentous Fungal Pathogens. In: Bolton, M., Thomma, B. (eds) Plant Fungal Pathogens. Methods in Molecular Biology, vol 835. Humana Press. https://doi.org/10.1007/978-1-61779-501-5_21

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  • DOI: https://doi.org/10.1007/978-1-61779-501-5_21

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-500-8

  • Online ISBN: 978-1-61779-501-5

  • eBook Packages: Springer Protocols

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