Abstract
Neuroproteomics is a branch of proteomics that specifically studies qualitatively and/or quantitatively the tissue/organelle proteomes of the nervous system. This chapter introduces the various aspects of neuroproteomics, and outlines the range of methods that are commonly employed.
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Guitart, X., and Nestler, E. J. (1989) Identification of morphine- and cyclic AMP-regulated phosphoproteins (MARPPs) in the locus coeruleus and other regions of rat brain: regulation by acute and chronic morphine, J Neurosci 9, 4371–4387.
Castellucci, V. F., Kennedy, T. E., Kandel, E. R., and Goelet, P. (1988) A quantitative analysis of 2-D gels identifies proteins in which labeling is increased following long-term sensitization in Aplysia, Neuron 1, 321–328.
Choudhary, C., and Mann, M. (2010) Decoding signalling networks by mass spectrometry-based proteomics, Nature reviews 11, 427–439.
Li, K. W., and Jimenez, C. R. (2008) Synapse proteomics: current status and quantitative applications, Expert review of proteomics 5, 353–360.
Lull, M. E., Freeman, W. M., VanGuilder, H. D., and Vrana, K. E. (2010) The use of neuroproteomics in drug abuse research, Drug and alcohol dependence 107, 11–22.
Li, K. W., and Smit, A. B. (2008) Subcellular proteomics in neuroscience, Front Biosci 13, 4416–4425.
Li, K. W., Klemmer, P., and Smit, A. B. (2010) Interaction proteomics of synapse protein complexes, Analytical and bioanalytical chemistry 397, 3195–3202.
Westman-Brinkmalm, A., Ruetschi, U., Portelius, E., Andreasson, U., Brinkmalm, G., Karlsson, G., Hansson, S., Zetterberg, H., and Blennow, K. (2009) Proteomics/peptidomics tools to find CSF biomarkers for neurodegenerative diseases, Front Biosci 14, 1793–1806.
Kovacech, B., Zilka, N., and Novak, M. (2009) New age of neuroproteomics in Alzheimer’s disease research, Cellular and molecular neurobiology 29, 799–805.
Bayes, A., and Grant, S. G. (2009) Neuroproteomics: understanding the molecular organization and complexity of the brain, Nat Rev Neurosci 10, 635–646.
Trinidad, J. C., Thalhammer, A., Specht, C. G., Lynn, A. J., Baker, P. R., Schoepfer, R., and Burlingame, A. L. (2008) Quantitative analysis of synaptic phosphorylation and protein expression, Mol Cell Proteomics 7, 684–696.
Van den Oever, M. C., Goriounova, N. A., Li, K. W., Van der Schors, R. C., Binnekade, R., Schoffelmeer, A. N., Mansvelder, H. D., Smit, A. B., Spijker, S., and De Vries, T. J. (2008) Prefrontal cortex AMPA receptor plasticity is crucial for cue-induced relapse to heroin-seeking, Nature neuroscience 11, 1053–1058.
Li, K. W., Miller, S., Klychnikov, O., Loos, M., Stahl-Zeng, J., Spijker, S., Mayford, M., and Smit, A. B. (2007) Quantitative proteomics and protein network analysis of hippocampal synapses of CaMKIIalpha mutant mice, Journal of proteome research 6, 3127–3133.
Hilger, M., Bonaldi, T., Gnad, F., and Mann, M. (2009) Systems-wide analysis of a phosphatase knock-down by quantitative proteomics and phosphoproteomics, Mol Cell Proteomics 8, 1908–1920.
Ye, J., Zhang, X., Young, C., Zhao, X., Hao, Q., Cheng, L., and Jensen, O. N. (2010) Optimized IMAC-IMAC Protocol for Phosphopeptide Recovery from Complex Biological Samples, Journal of proteome research 9, 3561–3573.
Tsai, C. F., Wang, Y. T., Chen, Y. R., Lai, C. Y., Lin, P. Y., Pan, K. T., Chen, J. Y., Khoo, K. H., and Chen, Y. J. (2008) Immobilized metal affinity chromatography revisited: pH/acid control toward high selectivity in phosphoproteomics, Journal of proteome research 7, 4058–4069.
Martin, S., Nishimune, A., Mellor, J. R., and Henley, J. M. (2007) SUMOylation regulates kainate-receptor-mediated synaptic transmission, Nature 447, 321–325.
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Li, K.W. (2011). Neuroproteomics: Deciphering Brain Function and Disorders. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 57. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-111-6_1
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DOI: https://doi.org/10.1007/978-1-61779-111-6_1
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-110-9
Online ISBN: 978-1-61779-111-6
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