Abstract
Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high-affinity probes for telomeric repeated sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.
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Acknowledgments
Work in the Londoño laboratory is supported by “La Ligue contre le Cancer,” the “Fondation pour la Recherche Medicale, FRM,” the “Association pour la Recherche sur le Cancer, ARC.” I. O-G. is the recipient of a post-doctoral fellowship from Agence Nationale de la Recherche (ANR) and ARC.
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Ourliac-Garnier, I., Londoño-Vallejo, A. (2011). Telomere Length Analysis by Quantitative Fluorescent In situ Hybridization (Q-FISH). In: Songyang, Z. (eds) Telomeres and Telomerase. Methods in Molecular Biology, vol 735. Humana Press. https://doi.org/10.1007/978-1-61779-092-8_3
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DOI: https://doi.org/10.1007/978-1-61779-092-8_3
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