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Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH)

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Telomeres and Telomerase

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1587))

Abstract

Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high affinity probes for telomeric repeat sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.

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Acknowledgments

Work in the Londoño laboratory is supported by “La Ligue contre le Cancer”, the “Fondation pour la Recherche Medicale, FRM”, the “Association pour la Recherche sur le Cancer, ARC”. I. O-G. was the recipient of a PhD fellowship from Agence Nationale de la Recherche (ANR) and ARC.

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Authors and Affiliations

  1. Telomeres & Cancer laboratory, CNRS-UMR3244, Institut Curie, 26 rue d’Ulm, 75248, Paris, France

    Isabelle Ourliac-Garnier & Arturo Londoño-Vallejo

  2. UPMC Univ. Paris 06, F-75005, Paris, France

    Isabelle Ourliac-Garnier & Arturo Londoño-Vallejo

Authors
  1. Isabelle Ourliac-Garnier
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  2. Arturo Londoño-Vallejo
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Corresponding author

Correspondence to Arturo Londoño-Vallejo .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA

    Zhou Songyang

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Ourliac-Garnier, I., Londoño-Vallejo, A. (2017). Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH). In: Songyang, Z. (eds) Telomeres and Telomerase. Methods in Molecular Biology, vol 1587. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6892-3_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6892-3_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6891-6

  • Online ISBN: 978-1-4939-6892-3

  • eBook Packages: Springer Protocols

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