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Multi-walled Carbon Nanotube (MWCNT) Synthesis, Preparation, Labeling, and Functionalization

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Immunotherapy of Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 651))

Abstract

Nanomedicine is a growing field with a great potential for introducing new generation of targeted and personalized drug. Amongst new generation of nano-vectors are carbon nanotubes (CNTs), which can be produced as single or multi-walled. Multi-walled carbon nanotubes (MWCNTs) can be fabricated as biocompatible nanostructures (cylindrical bulky tubes). These structures are currently under investigation for their application in nanomedicine as viable and safe nanovectors for gene and drug delivery. In this chapter, we will provide you with the necessary information to understand the synthesis of MWCNTs, functionalization, PKH26 labeling, RNAi, and DNA loading for in vitro experimentation and in vivo implantation of labeled MWCNT in mice as well as materials used in this experimentation. We used this technique to manipulate microglia as part of a novel application for the brain cancer immunotherapy. Our published data show this is a promising technique for labeling, and gene and drug delivery into microglia.

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Acknowledgments

Part of the research described in this publication was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration.

Author information

Authors and Affiliations

  1. Brain Mapping Foundation and International Brain Mapping & Intraoperative Surgical Planning Society (IBMISPS), West Hollywood, CA, USA

    Darya Alizadeh, Leying Zhang & Behnam Badie

  2. Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    Vicky Yamamoto

  3. Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, USA

    Harish M. Manohara & Michael J. Bronikowski

Authors
  1. Babak Kateb
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  2. Vicky Yamamoto
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  3. Darya Alizadeh
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  4. Leying Zhang
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  5. Harish M. Manohara
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  6. Michael J. Bronikowski
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  7. Behnam Badie
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Editor information

Editors and Affiliations

  1. , Center for Cell and Gene Therapy, Baylor College of Medicine, Baylor Plaza One, Houston, 77030, USA

    Patricia Yotnda

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Kateb, B. et al. (2010). Multi-walled Carbon Nanotube (MWCNT) Synthesis, Preparation, Labeling, and Functionalization. In: Yotnda, P. (eds) Immunotherapy of Cancer. Methods in Molecular Biology, vol 651. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-786-0_18

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  • DOI: https://doi.org/10.1007/978-1-60761-786-0_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-785-3

  • Online ISBN: 978-1-60761-786-0

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