Summary
RNA interference (RNAi) is one of the most commonly used procedures for gene targeting in today’s cutting edge technology and has great potential for use in clinical therapy. Using a plasmid construct that exogenously expresses short-hairpin RNAs (shRNAs) targeting a desired gene transcript not only helps to study the downstream effects of a gene product but also offers an alternative to viral vectors for gene therapy. Using a plasmid vector to knockdown a gene allows for long-term and permanent gene knockdown, without the need to generate knockout genotypes. Here, we detail the methodology for constructing a plasmid targeting the human telomerase reverse transcriptase (hTERT) gene through RNAi using the Ambion pSilencer system.
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© 2007 Humana Press Inc.
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Lai, S.R., Andrews, L.G., Tollefsbol, T.O. (2007). RNA Interference Using a Plasmid Construct Expressing Short-Hairpin RNA. In: Andrews, L.G., Tollefsbol, T.O. (eds) Telomerase Inhibition. Methods in Molecular Biology™, vol 405. Humana Press. https://doi.org/10.1007/978-1-60327-070-0_4
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DOI: https://doi.org/10.1007/978-1-60327-070-0_4
Publisher Name: Humana Press
Print ISBN: 978-1-58829-683-2
Online ISBN: 978-1-60327-070-0
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