Abstract
SDS-PAGE is the most widely used method for qualitatively analyzing protein mixtures. It is particularly useful for monitoring protein purification, and because the method is based on the separation of proteins according to size, the method can also be used to determine the relative molecular mass of proteins (see Note 14).
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References
Deyl, Z. (1979) Electrophoresis: A Survey of Techniques and Applications. Part A Techniques. Elsevier, Amsterdam.
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Hames, B. D. and Rickwood, D. (eds.) (1990) Gel Electrophoresis of Proteins—A Practical Approach. IRL, Oxford University Press, Oxford.
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Walker, J.M. (2009). SDS Polyacrylamide Gel Electrophoresis of Proteins. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_21
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DOI: https://doi.org/10.1007/978-1-59745-198-7_21
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60327-474-6
Online ISBN: 978-1-59745-198-7
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